Antifungal activity of biosynthesized silver nanoparticles from Candida albicans on the strain lacking the CNP41 gene

The upsurge of immunocompromised patients has led to extensive study of fungal infections with Candida albicans being the frontline model of pathogenic yeast in humans. In the quest to find novel antifungal agents, this study reports the potential usage of wild-type C. albicans strain C86 to biosynthesise silver nanoparticles by microwave assisted technique. Visual colour change and UV-spectrophotometer were used for primary detection of silver nanoparticles. Additionally, the FTIR peaks confirm the particles' formation and surface characterisation techniques such as FESEM and EDX suggests that the silver nanoparticles were sized in the range of 30-70 nm. Furthermore, pioneering work of homologous recombination technique was systematically employed to delete uncharacterized gene orf19.3120 (CNP41) in the C86 strain creating the deletion strain C403 of C. albicans. To amalgamate the two significant findings, biosynthesized silver nanoparticles were subjected to antifungal studies by disk diffusion assay on the strain C403 that lacks the gene orf19.3120 (CNP41) of C. albicans. As a synergetic approach, combinational effect was studied by incorporating antifungal drug fluconazole. Both individual and enhanced combinational antifungal effects of silver nanoparticles and fluconazole were observed on genetically modified C403 strain with 40% increase in fold area compared to wild-type C86 strain. This can be attributed to the synergetic effect of the bonding reaction between fluconazole and AgNPs. Taken together, this first-ever interdisciplinary study strongly suggests that the CNP41 gene could play a vital role in drug resistance in this fungal pathogen.publishersversionpublishe

Isotherm, kinetic and modeling studies

Funding Information: Funding: The Deanship of Scientific Research at King Khalid University General Research Project under the grant number (R.G.P.2/138/42) and Taif University researchers supporting project number (TURSP–2020/157), Taif University, Taif, Saudi Arabia. Funding Information: Acknowledgments: The co‐author Ali E. Anqi would like to extend his appreciation to the Deanship of Scientific Research at King Khalid University for the support he received through General Re‐ search Project under the grant number (R.G.P.2/138/42). This work was supported by Taif Univer‐ sity researchers supporting project number (TURSP–2020/157), Taif University, Taif, Saudi Arabia. The first author was thankful to the Directorate of Minorities, Govt. of Karnataka for providing PhD fellowship to conduct the research. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The first-ever use of halloysite nanotube (HNT), a relatively low-cost nanomaterial abun-dantly available with minor toxicity for removing brilliant green dye from aqueous media, is re-ported. The factors affecting adsorption were studied by assessing the adsorption capacity, kinetics, and equilibrium thermodynamic properties. All the experiments were designed at a pH level of around 7. The Redlich-Peterson isotherm model fits best amongst the nine isotherm models studied. The kinetic studies data confirmed a pseudo model of the second order. Robotic investigations pro-pose a rate-controlling advance being overwhelmed by intraparticle dispersion. The adsorbent fea-tures were interpreted using infrared spectroscopy and electron microscopy. Process optimization was carried out using Response Surface Methodology (RSM) through a dual section Fractional Fac-torial Experimental Design to contemplate the impact of boundaries on the course of adsorption. The examination of fluctuation (ANOVA) was utilized to consider the joined impact of the boundaries. The possibilities of the use of dye adsorbing HNT (“sludge”) for the fabrication of the composites using plastic waste are suggested.publishersversionpublishe

A recent study on remediation of direct blue 15 dye using halloysite nanotubes

R.G.P.2/138/42 TURSP–2020/157A set of lab‐scale experiments were designed and conducted to remedy Direct Blue 15 (DB15) dye using nontoxic halloysite nanotubes (HNT) with the view to be utilized in a textile industrial effluent (TIE). The DB15 adsorbed‐HNT “sludge” was used as a reinforcing agent and plas-tic waste to fabricate the composite. To advance the knowledge and further understand the chemical phenomena associated with DB15 adsorption on HNT, different factors like pH value, adsorbate initial concentration, adsorbent dosage, and temperature on the composite were affected experi-mentally tested. To estimate the adsorption capacity of HNT, nine isotherm models were applied, and it was identified that the Brouers–Sotolongo adsorption isotherm model represented the best accuracy for predicting the adsorption behavior of the HNT. Likewise, the pseudo‐second‐order reaction was the predominant mechanism for the overall rate of the multi‐step dye adsorption pro-cess. Additionally, it was demonstrated that the mass transfer during the process is diffusion‐con-trolled, and thermodynamic assessments showed that the process is physisorption.publishersversionpublishe

Clinical profile and treatment of infantile spasms using vigabatrin and ACTH - a developing country perspective

Background: Infantile spasms represent a serious epileptic syndrome that occurs in the early infantile age. ACTH and Vigabatrin are actively investigated drugs in its treatment. This study describes the comparison of their efficacy in a large series of Patients with infantile spasms from Pakistan. Methods: All Patients with infantile spasms who presented to Aga Khan University Hospital, Karachi, Pakistan from January, 2006 to April, 2008 were included in this study. Inclusion criteria were clinical symptoms of infantile spasms, hypsarrythmia or modified hyparrythmia on electroencephalography, at least six months of follow-up period and receipt of any of the two drugs mentioned above. The type of drug distribution was random according to the availability, cost and ease of administration. Results: Fifty six cases fulfilled the inclusion criteria. 62.5% were males. Mean age at onset of seizures was 5 +/- 1.4 months. Fifty two (92.8%) Patients demonstrated hypsarrythmia on electroencephalography. 64.3% cases were identified as symptomatic while 19.6% were cryptogenic and 16.1% were idiopathic. Eighteen Patients received ACTH while 38 Patients received Vigabatrin as first line therapy. Initial response to first line therapy was similar (50% for ACTH and 55.3% for Vigabatrin). Overall, the symptomatic and idiopathic groups responded better to Vigabatrin. The relapse rate was higher for ACTH as compared to Vigabatrin (55.5% vs. 33.3%) when considering the first line therapy. Four Patients evolved to Lennox-Gastaut variant, all of these Patients had initially received Vigabatrin and then ACTH. Conclusion: Vigabatrin and ACTH showed no significant difference in the initial treatment of infantile spasms. However, Patients receiving ACTH were 1.2 times more likely to relapse as compared to the Patients receiving Vigabatrin when considering monotherapy. We suggest that Vigabatrin should be the initial drug of choice in Patients presenting with infantile spasms. However, larger studies from developing countries are required to validate the therapeutic trends observed in this study

Impact of today's media on university student's body image in Pakistan: a conservative, developing country's perspective

<p>Abstract</p> <p>Background</p> <p>Living in a world greatly controlled by mass media makes it impossible to escape its pervading influence. As media in Pakistan has been free in the true sense of the word for only a few years, its impact on individuals is yet to be assessed. Our study aims to be the first to look at the effect media has on the body image of university students in a conservative, developing country like Pakistan. Also, we introduced the novel concept of body image dissatisfaction as being both negative and positive.</p> <p>Methods</p> <p>A cross-sectional study was conducted among 7 private universities over a period of two weeks in the city of Karachi, Pakistan's largest and most populous city. Convenience sampling was used to select both male and female undergraduate students aged between 18 and 25 and a sample size of 783 was calculated.</p> <p>Results</p> <p>Of the 784 final respondents, 376 (48%) were males and 408 (52%) females. The mean age of males was 20.77 (+/- 1.85) years and females was 20.38 (+/- 1.63) years. Out of these, 358 (45.6%) respondents had a positive BID (body image dissatisfaction) score while 426 (54.4%) had a negative BID score. Of the respondents who had positive BID scores, 93 (24.7%) were male and 265 (65.0%) were female. Of the respondents with a negative BID score, 283 (75.3%) were male and 143 (35.0%) were female. The results for BID vs. media exposure were similar in both high and low peer pressure groups. Low media exposure meant positive BID scores and vice versa in both groups (p < 0.0001) showing a statistically significant association between high media exposure and negative body image dissatisfaction. Finally, we looked at the association between gender and image dissatisfaction. Again a statistically significant association was found between positive body image dissatisfaction and female gender and negative body image dissatisfaction and male gender (p < 0.0001).</p> <p>Conclusions</p> <p>Our study confirmed the tendency of the media to have an overall negative effect on individuals' body image. A striking feature of our study, however, was the finding that negative body image dissatisfaction was found to be more prevalent in males as compared to females. Likewise, positive BID scores were more prevalent amongst females.</p

Photosensitized production of hydrogen by Halobacterium halobium MMT<SUB>22</SUB> coupled to Escherichia coli in reversed micelles of sodium lauryl sulfate in organic solvents

Observations on the enhanced production of hydrogen by Halobacterium halobium MMT<SUB>22</SUB> coupled to Escherichia coli entrapped inside the reversed micelles formed by sodium lauryl sulfate in various organic solvents, namely benzene, carbon tetrachloride, toluene, n-heptane, nitrobenzene, chlorobenzene, are reported. In the present system, a hundred fold increase in activity as compared to the activity in the usual aqueous medium was observed