A Dual Read-Out Assay to Evaluate the Potency of Compounds Active against Mycobacterium tuberculosis

PMCID: PMC3617142This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Recommended cereal varieties : 1969

Cereal varieties widely recommended for growing in 1969 are Gamenya and Falcon wheats, Dampier and Beecher barleys and Swan oats. Other varieties include the new wheat Darkan, which is recommended only for the higher rainfall areas, the rust resistant wheats Mengavi and Gamut, and Irwin oats for late sowing in northeastern districts

Asymmetric Fermi superfluid with different atomic species in a harmonic trap

We study the dilute fermion gas with pairing between two species and unequal concentrations in a harmonic trap using the mean field theory and the local density approximation. We found that the system can exhibit a superfluid shell structure sandwiched by the normal fermions. This superfluid shell structure occurs if the mass ratio is larger then certain critical value which increases from the weak-coupling BCS region to the strong-coupling BEC side. In the strong coupling BEC regime, the radii of superfluid phase are less sensitive to the mass ratios and are similar to the case of pairing with equal masses. However, the lighter leftover fermions are easier to mix with the superfluid core than the heavier ones. A partially polarized superfluid can be found if the majority fermions are lighter, whereas phase separation is still found if they are heavier.Comment: 12 pages, 7 figure

Chronic viral infection promotes sustained Th1-derived immunoregulatory IL-10 via BLIMP-1

During the course of many chronic viral infections, the antiviral T cell response becomes attenuated through a process that is regulated in part by the host. While elevated expression of the immunosuppressive cytokine IL-10 is involved in the suppression of viral-specific T cell responses, the relevant cellular sources of IL-10, as well as the pathways responsible for IL-10 induction, remain unclear. In this study, we traced IL-10 production over the course of chronic lymphocytic choriomeningitis virus (LCMV) infection in an IL-10 reporter mouse line. Using this model, we demonstrated that virus-specific T cells with reduced inflammatory function, particularly Th1 cells, display elevated and sustained IL-10 expression during chronic LCMV infection. Furthermore, ablation of IL-10 from the T cell compartment partially restored T cell function and reduced viral loads in LCMV-infected animals. We found that viral persistence is needed for sustained IL-10 production by Th1 cells and that the transcription factor BLIMP-1 is required for IL-10 expression by Th1 cells. Restimulation of Th1 cells from LCMV-infected mice promoted BLIMP-1 and subsequent IL-10 expression, suggesting that constant antigen exposure likely induces the BLIMP-1/IL-10 pathway during chronic viral infection. Together, these data indicate that effector T cells self-limit their responsiveness during persistent viral infection via an IL-10-dependent negative feedback loop.This work was supported by an Australian NHMRC Overseas Biomedical Postdoctoral Fellowship (to I.A. Parish); a Yale School of Medicine Brown-Coxe Postdoctoral Fellowship (to I.A. Parish); the Alexander von Humboldt Foundation (SKA2010, to P.A. Lang); a CIHR grant (to P.S. Ohashi); and by the Howard Hughes Medical Institute and NIH grant RO1AI074699 (to S.M. Kaech). P.S. Ohashi holds a Canada Research Chair in Autoimmunity and Tumor immunity