School based oral health education

Data sources: The methodology followed the Cochrane Handbook for systematic reviews of interventions with MEDLINE/PubMed, CENTRAL, Embase and LILACS databases searched. Publication date was limited to 1995-2015 with no restriction on language. Study selection: Two independent reviewers selected randomised controlled clinical trials involving oral health education provided by a dental care professional to children aged between five and 18 years old within a school setting. Eligible studies were those which had outcomes including caries, plaque accumulation, gingivitis, toothache or tooth loss. Randomisation was at group (school and/or classroom) or individual level. The control groups were not provided with an educational programme on oral health, however they could have been given an action that belonged to the school's curricular framework. Data extraction and synthesis: The title and abstract of each study was reviewed and critically assessed by two independent reviewers. Risk of bias was assessed using the Cochrane Handbook. Studies where the data of interest were presented in charts or were of dichotomous data were not included in meta-analysis. Results: Twelve studies were included in this systematic review. Five studies showed plaque level reduction in the intervention groups and two studies found no effect of the interventions on gingivitis. There was insufficient evidence on effectiveness of the interventions in reducing dental caries. Conclusions: Traditional oral health educational actions were effective in reducing plaque in the short-term, but not gingivitis. There was no long-term evidence regarding the effectiveness of traditional oral health educational actions in the school environment on preventing plaque accumulation, gingivitis and dental caries in schoolchildren

The burden of dental care in Amelogenesis Imperfecta paediatric patients in the UK NHS: a retrospective, multi-centred analysis

PURPOSE: The burden of dental care in Amelogenesis Imperfecta (AI) has not been well described. This condition results in weak, discoloured and often sensitive teeth. Specialist paediatric care is available for AI patients in the UK, but treatment protocols and care provided are inconsistent. The aim of this study was therefore to analyse the provision of treatment and burden of care for children and families with AI across four Paediatric Dentistry centres in the UK. METHODS: A retrospective evaluation of AI patient clinical records across four UK consultant-led Paediatric Dentistry centres was completed. Frequency and duration of care were recorded along with treatment and experience of inhalation sedation, local and general anaesthetic. RESULTS: In total, 138 records were available for analysis. The average patient age at first referral was 7.7 years (range 1-16 years) and families travelled an average 21.8 miles per appointment (range 0.2-286 miles). Patients attended on average 4.5 appointments per year for 5.8 years. In total, 65.2% had experience of local anaesthetic, 27.5% inhalation sedation and 31.9% general anaesthetic. Dental treatment including restorations and extractions were commonly required on multiple teeth per patient. CONCLUSION: AI carries a high burden of specialist dental care to patients and families. Specialist centres are required to provide longitudinal, comprehensive care

A Cylindrical GEM Inner Tracker for the BESIII experiment at IHEP

The Beijing Electron Spectrometer III (BESIII) is a multipurpose detector that collects data provided by the collision in the Beijing Electron Positron Collider II (BEPCII), hosted at the Institute of High Energy Physics of Beijing. Since the beginning of its operation, BESIII has collected the world largest sample of J/{\psi} and {\psi}(2s). Due to the increase of the luminosity up to its nominal value of 10^33 cm-2 s-1 and aging effect, the MDC decreases its efficiency in the first layers up to 35% with respect to the value in 2014. Since BESIII has to take data up to 2022 with the chance to continue up to 2027, the Italian collaboration proposed to replace the inner part of the MDC with three independent layers of Cylindrical triple-GEM (CGEM). The CGEM-IT project will deploy several new features and innovation with respect the other current GEM based detector: the {\mu}TPC and analog readout, with time and charge measurements will allow to reach the 130 {\mu}m spatial resolution in 1 T magnetic field requested by the BESIII collaboration. In this proceeding, an update of the status of the project will be presented, with a particular focus on the results with planar and cylindrical prototypes with test beams data. These results are beyond the state of the art for GEM technology in magnetic field

Identification of particles with Lorentz factor up to 10410^{4} with Transition Radiation Detectors based on micro-strip silicon detectors

This work is dedicated to the study of a technique for hadron identification in the TeV momentum range, based on the simultaneous measurement of the energies and of the emission angles of the Transition Radiation (TR) X-rays with respect to the radiating particles. A detector setup has been built and tested with particles in a wide range of Lorentz factors (from about $10^3$ to about $4 \times 10^4$ crossing different types of radiators. The measured double-differential (in energy and angle) spectra of the TR photons are in a reasonably good agreement with TR simulation predictions.Comment: 31 pages, 12 figures, paper published on Nuclear Instruments & Methods

Environmental disturbance confounds prenatal glucocorticoid programming experiments in Wistar rats

Abstract Low birth weight in humans is predictive of hypertension in adult life, and while the mechanisms underlying this link remain unknown, fetal overexposure to glucocorticoids has been implicated. We have previously shown that prenatal dexamethasone (DEX) exposure in the rat lowers birth weight and programmes adult hypertension. This current study aimed to unravel the molecular nature of this hypertension. However, unknowingly, post hoc investigations revealed that our animals had been subjected to environmental noise stresses from an adjacent construction site, which were sufficient to confound our prenatal DEX-programming experiments. This perinatal stress successfully established low birth weight, hypercorticosteronaemia, insulin resistance, hypertension and hypothalamic-pituitary -adrenal axis dysfunction in vehicle (VEH)-treated offspring, such that the typical distinctions between both treatment groups were ameliorated. The lack of an additional effect on DEX-treated offspring is suggestive of a maximal effect of perinatal stress and glucocorticoids, serving to prevent against the potentially detrimental effects of sustained glucocorticoid hyper-exposure. Finally, this paper serves to inform researchers of the potential detrimental effects of neighbouring construction sites to their experiments. As research institutions expand and respond to everchanging building and animal welfare regulations, they are required to undergo either new construction and/or renovation. However, such structural changes are associated with a plethora of nuisances, such as noise and vibration, with each disturbance being capable of powerfully stimulating the hypothalamic -pituitary -adrenal (HPA) axis. 1 The auditory system is permanently open -even during sleep. Its rapid and overshooting excitations in response to noise signals are subcortically connected, via the amygdala, to the HPA axis, resulting in corticotrophin releasing hormone and adrenocorticotrophic hormone release. 2 Animal experiments show noise-induced changes in the sensitivity of glucocorticoid receptor (GR) by increase of heat-shock proteins 3 and ultrastructural changes of the adrenal gland. 4 Increased cortisol levels have been found in humans when exposed to aircraft 5 or road traffic noise, 6 even during sleep, 7 implying these effects are mainly without mental control. Of course, increased glucocorticoid and sympathetic neural secretion is a perfect short-term stress response, coordinating appropriate metabolic and vascular changes, and thereby assisting the individual to negotiate the stressor. However, over prolonged time periods, such as persistent noise-induced stress responses, can be gravely damaging to health. 2,8 Numerous animal studies have documented the programming effects of pre-and postnatal stress on offspring physiology and behaviour, which are remarkably analogous to those induced by fetal glucocorticoid overexposure. Exposing pregnant dams to stressful stimuli results in both maternal and fetal HPA activation. 9,10 Moreover, these offspring display an activated HPA axis till weaning, as adults are more anxious and stress-responsive

Progress towards the first measurement of charm baryon dipole moments

Electromagnetic dipole moments of short-lived particles are sensitive to physics within and beyond the Standard Model of particle physics but have not been accessible experimentally to date. To perform such measurements it has been proposed to exploit the spin precession of channeled particles in bent crystals at the LHC. Progress that enables the first measurement of charm baryon dipole moments is reported. In particular, the design and characterization on beam of silicon and germanium bent crystal prototypes, the optimization of the experimental setup, and advanced analysis techniques are discussed. Sensitivity studies show that first measurements of Λ+c and Ξ+c baryon dipole moments can be performed in two years of data taking with an experimental setup positioned upstream of the LHCb detector. Figur

Software-Defined Networks for Future Networks and Services: Main Technical Challenges and Business Implications

In 2013, the IEEE Future Directions Committee (FDC) formed an SDN work group to explore the amount of interest in forming an IEEE Software-Defined Network (SDN) Community. To this end, a Workshop on "SDN for Future Networks and Services" (SDN4FNS'13) was organized in Trento, Italy (Nov. 11th-13th 2013). Following the results of the workshop, in this paper, we have further analyzed scenarios, prior-art, state of standardization, and further discussed the main technical challenges and socio-economic aspects of SDN and virtualization in future networks and services. A number of research and development directions have been identified in this white paper, along with a comprehensive analysis of the technical feasibility and business availability of those fundamental technologies. A radical industry transition towards the "economy of information through softwarization" is expected in the near future

A Fourth KLK4 Mutation Is Associated with Enamel Hypomineralisation and Structural Abnormalities

“Amelogenesis imperfecta” (AI) describes a group of genetic conditions that result in defects in tooth enamel formation. Mutations in many genes are known to cause AI, including the gene encoding the serine protease, kallikrein related peptidase 4 (KLK4), expressed during the maturation stage of amelogenesis. In this study we report the fourth KLK4 mutation to be identified in autosomal recessively-inherited hypomaturation type AI, c.632delT, p.(L211Rfs*37) (NM_004917.4, NP_004908.4). This homozygous variant was identified in five Pakistani AI families and is predicted to result in a transcript with a premature stop codon that escapes nonsense mediated decay. However, the protein may misfold, as three of six disulphide bonds would be disrupted, and may be degraded or non-functional as a result. Primary teeth were obtained from one affected individual. The enamel phenotype was characterized using high-resolution computerized X-ray tomography (CT), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and microhardness testing (MH). Enamel from the affected individual (referred to as KLK4 enamel) was hypomineralised in comparison with matched control enamel. Furthermore, KLK4 inner enamel was hypomineralised compared with KLK4 outer enamel. SEM showed a clear structural demarcation between KLK4 inner and outer enamel, although enamel structure was similar to control tissue overall. EDX showed that KLK4 inner enamel contained less calcium and phosphorus and more nitrogen than control inner enamel and KLK4 outer enamel. MH testing showed that KLK4 inner enamel was significantly softer than KLK4 outer enamel (p < 0.001). However, the hardness of control inner enamel was not significantly different to that of control outer enamel. Overall, these findings suggest that the KLK4 c.632delT mutation may be a common cause of autosomal recessive AI in the Pakistani population. The phenotype data obtained mirror findings in the Klk4‾/‾ mouse and suggest that KLK4 is required for the hardening and mineralization of the inner enamel layer but is less essential for hardening and mineralization of the outer enamel layer

Timing the multiple cell death pathways initiated by Rose Bengal acetate photodynamic therapy

Rose Bengal acetate photodynamic therapy (RBAc–PDT) induced multiple cell death pathways in HeLa cells through ROS and ER stress. Indeed, apoptosis was the first preferred mechanism of death, and it was triggered by at least four different pathways, whose independent temporal activation ensures cell killing when one or several of the pathways are inactivated. Apoptosis occurred as early as 1 h after PDT through activation of intrinsic pathways, followed by activation of extrinsic, caspase-12-dependent and caspase-independent pathways, and by autophagy. The onset of the different apoptotic pathways and autophagy, that in our system had a pro-death role, was timed by determining the levels of caspases 9, 8, 3 and 12; Bcl-2 family; Hsp70; LC3B; GRP78 and phospho-eIF2α proteins. Interestingly, inhibition of one pathway, that is, caspase-9 (Z-LEHD-FMK), caspase-8 (Z-IETD-FMK), pan-caspases (Z-VAD-FMK), autophagy (3-MA) and necrosis (Nec-1), did not impair the activation of the others, suggesting that the independent onset of the different apoptotic pathways and autophagy did not occur in a subordinated manner. Altogether, our data indicate RBAc as a powerful photosensitiser that induces a prolonged cytotoxicity and time-related cell death onset by signals originating from or converging on almost all intracellular organelles. The fact that cancer cells can die through different mechanisms is a relevant clue in the choice and design of anticancer PDT

Defects in the acid phosphatase ACPT cause recessive hypoplastic amelogenesis imperfecta

We identified two homozygous missense variants (c.428C>T, p.(T143M) and c.746C>T, p.(P249L)) in ACPT, the gene encoding Acid Phosphatase, Testicular, which segregate with hypoplastic Amelogenesis imperfecta (AI) in two unrelated families. ACPT is reported to play a role in odontoblast differentiation and mineralisation by supplying phosphate during dentine formation. Analysis by computerised tomography and scanning electron microscopy of a primary molar tooth from an individual homozygous for the c.746C>T variant, revealed an enamel layer that was hypoplastic but mineralised with prismatic architecture. These findings implicate variants in ACPT as a cause of early failure of amelogenesis during the secretory phase