Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit

Due to an increasing risk of chemical contamination upon the application of synthetic fungicides to preserve fresh fruits and vegetables, essential oils are gaining increasing attentions. In this research, besides chemical analysis of the essential oils of three Satureja species (S. hortensis, S. spicigera, and S. khuzistanica) by GC-MS, their fungicidal and/or fungistatic effects on postharvest pathogens of strawberry were investigated. Essential oils were extracted by means of hydro-distillation and afterwards GC/MS analysis was performed to identify their components. Carvacrol, γ-terpinene and p-cymene were detected as the repeating main constituents of the spices, while thymol and carvacrol methyl ether were found as major components only in S. spicigera oil. In vitro results showed that at the maximum concentration, the essential oils did not possess fungicidal effects on Aspergillus niger but they exhibited fungicidal activities against Penicillium digitatum, Botrytis cinerea and Rhizopus stolonifer. However, S. khuzistanica was the strongest oil in fungicidal activity. S. hortensis oil was more effective than S. spicigera against B. cinerea whereas S. spicigera oil showed stronger fungicidal activity against R. stolonifer. In conclusion, essential oils isolated from three savory species could be suitable for applications in the food industry to control molds and improve the safety of fruits and vegetables. © 2015 Elsevier B.V

Resilience to heat in public space: a case study of Adelaide, South Australia

During summer heatwaves, heat load exacerbates in urban heat islands (especially in hot climates) and threatens public life in cities. This paper examines the links between urban microclimates, outdoor thermal discomfort and public life through an exploratory case study. Heat resilience is highlighted as the ability of the space to support its normal activities when experiencing out-of-comfort temperatures. It also reports on the correlations between heat sensitive outdoor activities and urban greenery in three disparate case studies in Adelaide. Results indicate that necessary and optional activities start to decline after the apparent temperature reaches the threshold of 28 °C–32 °C, while activities in public spaces with more urban greenery show higher resilience to heat stress. Research findings propose heat resilience as a quality indicator in public space and support the application of urban greenery to make urban settings more resilient to heat stress.Ehsan Sharifi, Alpana Sivam and John Bolan

Reduced Phagocytic Capacity of Blood Monocyte/Macrophages in Tuberculosis Patients Is Further Reduced by Smoking.

Tuberculosis (TB) and tobacco use are two major alarming global health issues posing immense threats to human populations. Mycobacterium tuberculosis (MTB) by activation of macrophages could induce the sequences of cells activation and releases of inflammatory cytokines such as CXCL-8, Il-12 and TNF-α which in turn induces the immune system network. However no information is available on other activity of cells by MTB and smoking. In the current study we aimed to investigate the serum levels TNF-a, CXCL-8 and phagocytosis capacity in tuberculosis patients with and without smoking. 103 subjects entered the study including 61 new diagnosed pulmonary TB patients (23 smokers and 38 nonsmokers) and 42 control healthy subjects. The phagocytosis of fluorescein isothiocyanate dextran (FITC-dextran) in blood monocytes/macrophages through flowcytometry was assessed. Serum levels of TNF-a and CXCL-8 were analyzed by ELISA methods. A lower percentage of cells from TB patients who smoked [50.29% (43.4-57.2), p<0.01] took up FITC-dextran after 2h compared to non-smoking TB subjects [71.62% (69.2-74.1)] and healthy cases [97.45% (95.9-99.1). Phagocytic capacity was inversely correlated with cigarette smoking as measured by pack years (r=-0.73, p<0.001). The serum levels of TNF-a and CXCL-8 were significantly higher in the TB patients who smoked compared to the TB non-smoker group (p<0.001, p<0.01 respectively). Blood monocytes/macrophages from TB patients have reduced phagocytic capacity which is further reduced in TB patients who smoke. Smoking enhanced serum levels of TNF-a and CXCL-8 suggesting a greater imbalance between the proinflammatory and anti-inflammatory factors in these patients