172 research outputs found
Metabolic pathways inferred from a bacterial marker gene illuminate ecological changes across South Pacific frontal boundaries
Global oceanographic monitoring initiatives originally measured abiotic essential ocean variables but are currently incorporating biological and metagenomic sampling programs. There is, however, a large knowledge gap on how to infer bacterial functions, the information sought by biogeochemists, ecologists, and modelers, from the bacterial taxonomic information (produced by bacterial marker gene surveys). Here, we provide a correlative understanding of how a bacterial marker gene (16S rRNA) can be used to infer latitudinal trends for metabolic pathways in global monitoring campaigns. From a transect spanning 7000âkm in the South Pacific Ocean we infer ten metabolic pathways from 16S rRNA gene sequences and 11 corresponding metagenome samples, which relate to metabolic processes of primary productivity, temperature-regulated thermodynamic effects, coping strategies for nutrient limitation, energy metabolism, and organic matter degradation. This study demonstrates that low-cost, high-throughput bacterial marker gene data, can be used to infer shifts in the metabolic strategies at the community scale
Weather in stellar atmosphere: the dynamics of mercury clouds in alpha Andromedae
The formation of long-lasting structures at the surfaces of stars is commonly
ascribed to the action of strong magnetic fields. This paradigm is supported by
observations of evolving cool spots in the Sun and active late-type stars, and
stationary chemical spots in the early-type magnetic stars. However, results of
our seven-year monitoring of mercury spots in non-magnetic early-type star
alpha Andromedae show that the picture of magnetically-driven structure
formation is fundamentally incomplete. Using an indirect stellar surface
mapping technique, we construct a series of 2-D images of starspots and
discover a secular evolution of the mercury cloud cover in this star. This
remarkable structure formation process, observed for the first time in any
star, is plausibly attributed to a non-equilibrium, dynamical evolution of the
heavy-element clouds created by atomic diffusion and may have the same
underlying physics as the weather patterns on terrestrial and giant planets.Comment: 10 pages, 2 figures; to be published in Nature Physic
Magnetic fields & rotation periods of M dwarfs from SPIRou spectra
We present near-infrared spectropolarimetric observations of a sample of 43
weakly- to moderately-active M dwarfs, carried with SPIRou at the
Canada-France-Hawaii Telescope in the framework of the SPIRou Legacy Survey
from early 2019 to mid 2022. We use the 6700 circularly polarised spectra
collected for this sample to investigate the longitudinal magnetic field and
its temporal variations for all sample stars, from which we diagnose, through
quasi-periodic Gaussian process regression, the periodic modulation and
longer-term fluctuations of the longitudinal field. We detect the large-scale
field for 40 of our 43 sample stars, and infer a reliable or tentative rotation
period for 38 of them, using a Bayesian framework to diagnose the confidence
level at which each rotation period is detected. We find rotation periods
ranging from 14 to over 60d for the early-M dwarfs, and from 70 to 200d for
most mid- and late-M dwarfs (potentially up to 430d for one of them). We also
find that the strength of the detected large-scale fields does not decrease
with increasing period or Rossby number for the slowly rotating dwarfs of our
sample as it does for higher-mass, more active stars, suggesting that these
magnetic fields may be generated through a different dynamo regime than those
of more rapidly rotating stars. We also show that the large-scale fields of
most sample stars evolve on long timescales, with some of them globally
switching sign as stars progress on their putative magnetic cycles.Comment: MNRAS, in press (25 pages, 15 figures, 3 tables
Psychological characteristics of mothers of patients with anorexia nervosa: implications for treatment and prognosis
The magnetic field and multiple planets of the young dwarf AU~Mic
In this paper we present an analysis of near-infrared spectropolarimetric and
velocimetric data of the young M dwarf AU Mic, collected with SPIRou at the
Canada-France-Hawaii telescope from 2019 to 2022, mostly within the SPIRou
Legacy Survey. With these data, we study the large- and small-scale magnetic
field of AU Mic, detected through the unpolarized and circularly-polarized
Zeeman signatures of spectral lines. We find that both are modulated with the
stellar rotation period (4.86 d), and evolve on a timescale of months under
differential rotation and intrinsic variability. The small-scale field,
estimated from the broadening of spectral lines, reaches kG. The
large-scale field, inferred with Zeeman-Doppler imaging from Least-Squares
Deconvolved profiles of circularly-polarized and unpolarized spectral lines, is
mostly poloidal and axisymmetric, with an average intensity of G. We
also find that surface differential rotation, as derived from the large-scale
field, is 30% weaker than that of the Sun. We detect the radial
velocity (RV) signatures of transiting planets b and c, although dwarfed by
activity, and put an upper limit on that of candidate planet d, putatively
causing the transit-timing variations of b and c. We also report the detection
of the RV signature of a new candidate planet (e) orbiting further out with a
period of d, i.e., near the 4:1 resonance with b. The RV
signature of e is detected at 6.5 while those of b and c show up at
4, yielding masses of and
Earth masses for b and c, and a minimum mass of
Earth masses for e.Comment: MNRAS, in press (20 pages and 12 figures + 9 pages of supplementary
material
Data Descriptor: Systematic, continental scale temporal monitoring of marine pelagic microbiota by the Australian Marine Microbial Biodiversity Initiative
© 2018 Author(s). Sustained observations of microbial dynamics are rare, especially in southern hemisphere waters. The Australian Marine Microbial Biodiversity Initiative (AMMBI) provides methodologically standardized, continental scale, temporal phylogenetic amplicon sequencing data describing Bacteria, Archaea and microbial Eukarya assemblages. Sequence data is linked to extensive physical, biological and chemical oceanographic contextual information. Samples are collected monthly to seasonally from multiple depths at seven sites: Darwin Harbour (Northern Territory), Yongala (Queensland), North Stradbroke Island (Queensland), Port Hacking (New South Wales), Maria Island (Tasmania), Kangaroo Island (South Australia), Rottnest Island (Western Australia). These sites span ~30° of latitude and ~38° longitude, range from tropical to cold temperate zones, and are influenced by both local and globally significant oceanographic and climatic features. All sequence datasets are provided in both raw and processed fashion. Currently 952 samples are publically available for bacteria and archaea which include 88,951,761 bacterial (72,435 unique) and 70,463,079 archaeal (24,205 unique) 16 S rRNA v1-3 gene sequences, and 388 samples are available for eukaryotes which include 39,801,050 (78,463 unique) 18 S rRNA v4 gene sequences
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Digital transformation: A multidisciplinary perspective and future research agenda
Data Availability Statement: Data sharing not applicable to this article as no datasets were generated or analysed during the current study.Digital transformation has had an unprecedented influence on all sectors of business over the last decade. We are now entering an era characterized by the extensive digital transformation of businesses, society, and consumers. Therefore, digital transformation has become a pivotal focus for organizations across various sectors in recent years. Despite differing scholarly perspectives on the concept and elements of digital transformation, a consensus exists that it significantly impacts consumer decisions and necessitates organizational adaptation. Recent challenges such as the COVID-19 pandemic have further accelerated the need for digital transformation and its effects on consumers. This necessitates an editorial perspective on this most important topic to establish future research agenda encompassing the various dimensions of digital transformation. The purpose of this editorial perspective is to review research on digital transformation from a multidisciplinary viewpoint and provide insights into several key domainsâInternet-of-Things, social media, mobile apps, artificial intelligence, augmented and virtual reality, the metaverse, and corporate digital responsibilityâthat are poised to fuel the pace of digital transformation. Each domain is analyzed through a lens of introduction, role, importance, multifaceted impact, and conclusions. Future research directions are suggested
Childhood abdominal pain in primary care: design and patient selection of the HONEUR abdominal pain cohort
Background: Abdominal pain in children is a common complaint presented to the GP. However, the prognosis and prognostic factors of childhood abdominal pain are almost exclusively studied in referred children. This cohort study aims at describing prognosis and prognostic factors of childhood abdominal pain in primary care. In this paper we describe methods used for data-collection and determine possible selective recruitment. Methods/Design: We conducted an observational, prospective cohort study with a 1-year follow-up. From May 2004 to March 2006, 53 Dutch GPs recruited consecutive children aged 4-17 years with a new episode of abdominal pain not preceded by a consultation for this complaint in the previous 3 months. Participants filled in standardized questionnaires, and faeces and urine were sampled. To evaluate selective recruitment, the electronic medical records of participating GPs were retrospectively searched for eligible non-included children. Discussion: This study allows us to describe prognosis and prognostic factors of childhood abdominal pain in primary care. A total of 305 children were included of whom 142 (46.6%) met predefined criteria for chronic/recurrent abdominal pain at presentation; from the total group of eligible children identified from the electronic medical record, 27% were included. The included children were significantly younger than non-included children (mean age 8.49 and 9.20 years). In proportion to identified eligible children, significantly less children diagnosed with "gastroenteritis" (6.8%) and significantly more children with "generalized abdominal pain" (39%) were included compared to the 27% that was expected. This cohort represents young school-aged children consulting GPs for a new episode of abdominal pain, not diagnosed as gastroenteritis. Almost half of them fulfil the criteria for chronic abdominal pain at presentation
Administration of intrapulmonary sodium polyacrylate to induce lung injury for the development of a porcine model of early acute respiratory distress syndrome.
BACKGROUND: The loss of alveolar epithelial and endothelial integrity is a central component in acute respiratory distress syndrome (ARDS); however, experimental models investigating the mechanisms of epithelial injury are lacking. The purpose of the present study was to design and develop an experimental porcine model of ARDS by inducing lung injury with intrapulmonary administration of sodium polyacrylate (SPA). METHODS: The present study was performed at the Centre for Comparative Medicine, University of British Columbia, Vancouver, British Columbia. Human alveolar epithelial cells were cultured with several different concentrations of SPA; a bioluminescence technique was used to assess cell death associated with each concentration. In the anesthetized pig model (female Yorkshire X pigs (nâ=â14)), lung injury was caused in 11 animals (SPA group) by injecting sequential aliquots (5 mL) of 1% SPA gel in aqueous solution into the distal airway via a rubber catheter through an endotracheal tube. The SPA was dispersed throughout the lungs by manual bag ventilation. Three control animals (CON group) underwent all experimental procedures and measurements with the exception of SPA administration. RESULTS: The mean (± SD) ATP concentration after incubation of human alveolar epithelial cells with 0.1% SPA (0.92â±â0.27 ΌM/well) was approximately 15% of the value found for the background control (6.30â±â0.37 ΌM/well; pâ<â0.001). Elastance of the respiratory system (E RS) and the lung (E L) increased in SPA-treated animals after injury (pâ=â0.003 and pâ<â0.001, respectively). Chest wall elastance (E CW) did not change in SPA-treated animals. There were no differences in E RS, E L, or E CW in the CON group when pre- and post-injury values were compared. Analysis of bronchoalveolar lavage fluid showed a significant shift toward neutrophil predominance from before to after injury in SPA-treated animals (pâ<â0.001) but not in the CON group (pâ=â0.38). Necropsy revealed marked consolidation and congestion of the dorsal lung lobes in SPA-treated animals, with light-microscopy evidence of bronchiolar and alveolar spaces filled with neutrophilic infiltrate, proteinaceous debris, and fibrin deposition. These findings were absent in animals in the CON group. Electron microscopy of lung tissue from SPA-treated animals revealed injury to the alveolar epithelium and basement membranes, including intra-alveolar neutrophils and fibrin on the alveolar surface and intravascular fibrin (microthrombosis). CONCLUSIONS: In this particular porcine model, the nonimmunogenic polymer SPA caused a rapid exudative lung injury. This model may be useful to study ARDS caused by epithelial injury and inflammation
Mainstreaming microbes across biomes
Bacteria, fungi, and other microorganisms in the environment (i.e., environmental microbiomes) provide vital ecosystem services and affecthuman health. Despite their importance, public awareness of environmental microbiomes has lagged behind that of human microbiomes. A keyproblem has been a scarcity of research demonstrating the microbial connections across environmental biomes (e.g., marine, soil) and betweenenvironmental and human microbiomes. We show in the present article, through analyses of almost 10,000 microbiome papers and threeglobal data sets, that there are significant taxonomic similarities in microbial communities across biomes, but very little cross-biome researchexists. This disconnect may be hindering advances in microbiome knowledge and translation. In this article, we highlight current and potentialapplications of environmental microbiome research and the benefits of an interdisciplinary, cross-biome approach. Microbiome scientists needto engage with each other, government, industry, and the public to ensure that research and applications proceed ethically, maximizing thepotential benefits to society
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