19 research outputs found
Neon Lights Up a Controversy: the Solar Ne/O Abundance
The standard solar model was so reliable that it could predict the existence
of the massive neutrino. Helioseismology measurements were so precise that they
could determine the depth of the convection zone. This agreement between theory
and observation was the envy of all astrophysics -- until recently when
sophisticated three-dimensional hydrodynamic calculations of the solar
atmosphere reduced the metal content by a factor of almost two. Antia & Basu
(2005) suggested that a higher value of the solar neon abundance, Ne/O = 0.52,
would resolve this controversy. Drake & Testa (2005) presented strong evidence
in favor of this idea from a sample of 21 Chandra stars with enhanced values of
the neon abundance, Ne/O = 0.41. In this paper, we have analyzed solar active
region spectra from the archive of the Flat Crystal Spectrometer on Solar
Maximum Mission, a NASA mission from the 1980s, as well as full-Sun spectra
from the pioneering days of X-ray astronomy in the 1960s. These data seem
consistent with the standard neon-to-oxygen abundance value, Ne/O = 0.15
(Grevesse & Sauval 1998). If these results prove to be correct, than the
enhanced-neon hypothesis will not resolve the current controversy.Comment: submitted to ApJ Letter
Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth
Seamless tubes form intracellularly without cell–cell or autocellular junctions. Such tubes have been described across phyla, but remain mysterious despite their simple architecture. In Drosophila, seamless tubes are found within tracheal terminal cells, which have dozens of branched protrusions extending hundreds of micrometres. We find that mutations in multiple components of the dynein motor complex block seamless tube growth, raising the possibility that the lumenal membrane forms through minus-end-directed transport of apical membrane components along microtubules. Growth of seamless tubes is polarized along the proximodistal axis by Rab35 and its apical membrane-localized GAP, Whacked. Strikingly, loss of whacked (or constitutive activation of Rab35) leads to tube overgrowth at terminal cell branch tips, whereas overexpression of Whacked (or dominant-negative Rab35) causes formation of ectopic tubes surrounding the terminal cell nucleus. Thus, vesicle trafficking has key roles in making and shaping seamless tubes
Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux
SUMMARY Many unicellular tubes such as capillaries form lumens intracellularly, a process that is not well understood. Here we show that the cortical membrane organizer ERM-1 is required to expand the intracellular apical/lumenal membrane and its actin undercoat during single-cell C.elegans excretory canal morphogenesis. We characterize AQP-8, identified in an ERM-1 overexpression (ERM-1[++]) suppressor screen, as a canalicular aquaporin that interacts with ERM-1 in lumen extension in a mercury-sensitive manner, implicating water-channel activity. AQP-8 is transiently recruited to the lumen by ERM-1, co-localizing in peri-lumenal cuffs interspaced along expanding canals. An ERM-1[++]-mediated increase in the number of lumen-associated canaliculi is reversed by AQP-8 depletion. We propose that the ERM-1-AQP-8 interaction propels lumen extension by translumenal flux, suggesting a direct morphogenetic effect of water-channel-regulated fluid pressure
Shifting Networks and Community Identity at Tell Tayinat in the Iron I (ca. 12th to mid-10th Cent. BCE)
The end of the 13th and beginning of the 12th centuries B.C.E. witnessed the demise of the great territorial states of the Bronze Age and, with them, the collapse of the extensive interregional trade networks that fueled their wealth and power. The period that follows has historically been characterized as an era of cultural devolution marked by profound social and political disruption. This report presents the preliminary results of the Tayinat Archaeological Project (TAP) investigations of Iron I (ca. 12th to mid 10th century B.C.E.) contexts at Tell Tayinat, which would emerge from this putative Dark Age as Kunulua, royal capital of the Neo-Hittite kingdom of Palastin/Patina/Unqi. In contrast to the prevailing view, the results of the TAP investigations at Early Iron Age Tayinat reveal an affluent community actively interacting with a wide spectrum of regions throughout the eastern Mediterranean. The evidence from Tayinat also highlights the distinctively local, regional character of its cultural development and the need for a more nuanced treatment of the considerable regional variability evident in the eastern Mediterranean during this formative period, a treatment that recognizes the diversity of relational networks, communities, and cultural identities being forged in the generation of a new social and economic order
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c21orf59/kurly Controls Both Cilia Motility and Polarization
Cilia are microtubule-based projections that function in the movement of extracellular fluid. This requires cilia to be: (1) motile and driven by dynein complexes and (2) correctly polarized on the surface of cells, which requires planar cell polarity (PCP). Few factors that regulate both processes have been discovered. We reveal that C21orf59/Kurly (Kur), a cytoplasmic protein with some enrichment at the base of cilia, is needed for motility; zebrafish mutants exhibit characteristic developmental abnormalities and dynein arm defects. kur was also required for proper cilia polarization in the zebrafish kidney and the larval skin of Xenopus laevis. CRISPR/Cas9 coupled with homologous recombination to disrupt the endogenous kur locus in Xenopus resulted in the asymmetric localization of the PCP protein Prickle2 being lost in mutant multiciliated cells. Kur also makes interactions with other PCP components, including Disheveled. This supports a model wherein Kur plays a dual role in cilia motility and polarization