Sensitivity analysis of EUROSEM using Monte Carlo simulation II::the effect of rills and rock fragments

A sensitivity analysis of the surface and catchment characteristics in the European soil erosion model (EUROSEM) was carried out with special emphasis on rills and rock fragment cover. The analysis focused on the use of Monte Carlo simulation but was supplemented by a simple sensitivity analysis where input variables were increased and decreased by 10%. The study showed that rock fragments have a significant effect upon the static output parameters of total runoff, peak flow rate, total soil loss and peak sediment discharge, but with a high coefficient of variation. The same applied to the average hydrographs and sedigraphs although the peak of the graphs was associated with a low coefficient of variation. On average, however, the model was able to simulate the effect of rock fragment cover quite well. The sensitivity analysis through the Monte Carlo simulation showed that the model is particularly sensitive to changes in parameters describing rills and the length of the plane when no rock fragments are simulated but that the model also is sensitive to changes in the fraction of non-erodible material and interrill slope when rock fragments were embedded in the topsoil. For rock fragments resting on the surface, changes in parameter values did not affect model output significantly. The simple sensitivity analysis supported the findings from the Monte Carlo simulation and illustrates the importance when choosing input parameters to describe both rills and rock fragment cover when modelling with EUROSEM

Optimization of Graphene Sensors to Detect Biological Warfare Agents

The Defense Threat Reduction Agency and the Air Force Office of Scientific Research require sensors to detect biological agents better, faster, and cheaper. This research explores the use of graphene functionalized with single strand deoxyribonucleic acid (ssDNA) aptamers as a detection method for the Sterne strain of Bacillus anthracis. This research is interesting because of the unique electrical and chemical binding properties of graphene and its interaction with ssDNA and DNA. The interactions of graphene functionalized with ssDNA in response to B. anthracis spores, non-complimentary and complimentary DNA were explored in static and dynamic aqueous environments with indirect complimentary binding confirmation via Raman spectroscopy and atomic force microscopy. The results suggest aptamer functionalized graphene can be used to detect B. anthracis spores, non-complimentary and complimentary DNA sequences

Agenesis of the corpus callosum is associated with developmental delay and intellectual disability

A critical appraisal and clinical application of Romaniello R, Marelli S, Giorda R, et al. Clinical characterization, genetics, and long-term follow-up of a large cohort of patients with agenesis of the corpus callosum. Journal of Child Neurology, 2016;32(1):60-71. doi: 10.1177/0883073816664668

Investigating source areas of eroded sediments transported in concentrated overland flow using rare earth element tracers

Rare earth element oxides (REOs) have excellent potential for use as tracers in erosion studies. Using laboratory and field experiments we aimed to develop and test a simple application method for spreading REOs and to use REOs to determine the source of sediment to concentrated overland flow paths. Tracks left by farm machinery (tramlines) in fields act as concentrated flow paths, delivering a large amount of surface runoff and entrained sediment to the field boundary however little is known regarding where this sediment is sourced from. Two field experiments were conducted to investigate this, one looking at whether sediment is entrained from different points along the length of the tramline and one looking at the area over which sediment is entrained from either side of a tramline. Results from leaching tests and rainfall simulations clearly demonstrated the potential of this method for tracing sediment, there was very little movement of REOs through the soil profile and they were transported with sediment in overland flow. In the field experiment a large proportion of the sediment transported in the tramline originated within the first metre either side of it. However, the total area over which eroded sediment collects and flows into the tramline is potentially large with small amounts of sediment eroded from the between 4 and 6 m being transported in the tramline. REOs have an excellent potential for use as a tracer of soil erosion. They provide a quick and cheap method of identifying sources of eroded sediments and have good potential for use in determining erosion rates

Examining the Effect of Pore Size Distribution and Shape on Flow through Unsaturated Peat using Computer Tomography

The hydraulic conductivity of unsaturated peat soil is controlled by the air-filled porosity, pore size and geometric distribution as well as other physical properties of peat materials. This study investigates how the size and shape of pores affects the flow of water through peat soils. In this study we used X-ray Computed Tomography (CT), at 45μm resolution under 5 specific soil-water pressure head levels to provide 3-D, high-resolution images that were used to detect the inner pore structure of peat samples under a changing water regime. Pore structure and configuration were found to be irregular, which affected the rate of water transmission through peat soils. The 3-D analysis suggested that pore distribution is dominated by a single large pore-space. At low pressure head, this single large air-filled pore imparted a more effective flowpath compared to smaller pores. Smaller pores were disconnected and the flowpath was more tortuous than in the single large air-filled pore, and their contribution to flow was negligible when the single large pore was active. We quantify the pore structure of peat soil that affects the hydraulic conductivity in the unsaturated condition, and demonstrate the validity of our estimation of peat unsaturated hydraulic conductivity by making a comparison with a standard permeameter-based method. Estimates of unsaturated hydraulic conductivities were made for the purpose of testing the sensitivity of pore shape and geometry parameters on the hydraulic properties of peats and how to evaluate the structure of the peat and its affects on parameterization. We also studied the ability to quantify these factors for different soil moisture contents in order to define how the factors controlling the shape coefficient vary with changes in soil water pressure head. The relation between measured and estimated unsaturated hydraulic conductivity at various heads shows that rapid initial drainage, that changes the air-filled pore properties, creates a sharp decline in hydraulic conductivity. This is because the large pores readily lose water, the peat rapidly becomes less conductive and the flow path among pores, more tortuous

The effects of minimal tillage, contour cultivation and in-field vegetative barriers on soil erosion and phosphorus loss.

Runoff, sediment, total phosphorus and total dissolved phosphorus losses in overland flow were measured for two years on unbounded plots cropped with wheat and oats. Half of the field was cultivated with minimum tillage (shallow tillage with a tine cultivator) and half was conventionally ploughed. Within each cultivation treatment there were different treatment areas (TA). In the first year of the experiment, one TA was cultivated up and down the slope, one TA was cultivated on the contour, with a beetle bank acting as a vegetative barrier partway up the slope, and one had a mixed direction cultivation treatment, with cultivation and drilling conducted up and down the slope and all subsequent operations conducted on the contour. In the second year, this mixed treatment was replaced with contour cultivation. Results showed no significant reduction in runoff, sediment losses or total phosphorus losses from minimum tillage when compared to the conventional plough treatment, but there were increased losses of total dissolved phosphorus with minimum tillage. The mixed direction cultivation treatment increased surface runoff and losses of sediment and phosphorus. Increasing surface roughness with contour cultivation reduced surface runoff compared to up and down slope cultivation in both the plough and minimum tillage treatment areas, but this trend was not significant. Sediment and phosphorus losses in the contour cultivation treatment followed a very similar pattern to runoff. Combining contour cultivation with a vegetative barrier in the form of a beetle bank to reduce slope length resulted in a non-significant reduction in surface runoff, sediment and total phosphorus when compared to up and down-slope cultivation, but there was a clear trend towards reduced losses. However, the addition of a beetle bank did not provide a significant reduction in runoff, sediment losses or total phosphorus losses when compared to contour cultivation, suggesting only a marginal additional benefit. The economic implications for farmers of the different treatment options are investigated in order to assess their suitability for implementation at a field scale

Comparison of Algorithms and Parameterisations for Infiltration into Organic-Covered Permafrost Soils

Infiltration into frozen and unfrozen soils is critical in hydrology, controlling active layer soil water dynamics and influencing runoff. Few Land Surface Models (LSMs) and Hydrological Models (HMs) have been developed, adapted or tested for frozen conditions and permafrost soils. Considering the vast geographical area influenced by freeze/thaw processes and permafrost, and the rapid environmental change observed worldwide in these regions, a need exists to improve models to better represent their hydrology. In this study, various infiltration algorithms and parameterisation methods, which are commonly employed in current LSMs and HMs were tested against detailed measurements at three sites in Canada’s discontinuous permafrost region with organic soil depths ranging from 0.02 to 3 m. Field data from two consecutive years were used to calibrate and evaluate the infiltration algorithms and parameterisations. Important conclusions include: (1) the single most important factor that controls the infiltration at permafrost sites is ground thaw depth, (2) differences among the simulated infiltration by different algorithms and parameterisations were only found when the ground was frozen or during the initial fast thawing stages, but not after ground thaw reaches a critical depth of 15 to 30 cm, (3) despite similarities in simulated total infiltration after ground thaw reaches the critical depth, the choice of algorithm influenced the distribution of water among the soil layers, and (4) the ice impedance factor for hydraulic conductivity, which is commonly used in LSMs and HMs, may not be necessary once the water potential driven frozen soil parameterisation is employed. Results from this work provide guidelines that can be directly implemented in LSMs and HMs to improve their application in organic covered permafrost soils

Improved synchronous production of Plasmodium falciparum gametocytes in vitro.

The sexual stages of the Plasmodium falciparum life cycle are attractive targets for vaccines and transmission blocking drugs. Difficulties in culturing and obtaining large amounts of sexual stage P. falciparum parasites, particularly early stages, have often limited research progress in this area. We present a new protocol which simplifies the process of stimulating gametocytogenesis leading to improved synchronous gametocyte production. This new method can be adapted to enrich for early stage gametocytes (I and II) with a higher degree of purity than has previously been achieved, using MACS magnetic affinity columns. The protocol described lends itself to large scale culturing and harvesting of synchronous parasites suitable for biochemical assays, northern blots, flow cytometry, microarrays and proteomic analysis