Stone Soup: No Longer Just an Appetiser

This paper announces version 1.0 of Stone Soup: the open-source tracking and state estimation framework. We highlight key elements of the framework and outline example applications and community activities.Stone Soup is engineered with modularity and encapsulation at its heart. This means that its many components can be put together in any number of ways to build, compare, and assure almost any type of multi-target tracking and fusion algorithm. Since its inception in 2017, it has aimed to provide the target tracking and state estimation community with an open, easy-to-deploy framework to develop and assess the performance of different types of trackers. Now, through repeated application in many use cases, implementation of a wide variety of algorithms, multiple beta releases, and contributions from the community, the framework has reached a stable point.In announcing this release, we hope to encourage additional adoption and further contributions to the toolkit. We also acknowledge and express appreciation for the many contributions of time and expertise donated by the tracking and fusion community

Small artery remodeling depends on tissue-type transglutaminase

Remodeling of small arteries is essential in the long-term regulation of blood pressure and blood flow to specific organs or tissues. A large part of the change in vessel diameter may occur through non-growth-related reorganization of vessel wall components. The hypothesis was tested that tissue-type transglutaminase (tTG), a cross-linking enzyme, contributes to the inward remodeling of small arteries. The in vivo inward remodeling of rat mesenteric arteries, induced by low blood flow, was attenuated by inhibition of tTG. Rat skeletal muscle arteries expressed tTG, as identified by Western blot and immunostaining. In vitro, activation of these arteries with endothelin-1 resulted in inward remodeling, which was blocked by tTG inhibitors. Small arteries obtained from rats and pigs both showed inward remodeling after exposure to exogenous transglutaminase, which was inhibited by addition of a nitric oxide donor. Enhanced expression of tTG, induced by retinoic acid, increased inward remodeling of porcine coronary arteries kept in organ culture for 3 days. The activity of tTG was dependent on pressure. Inhibition of tTG reversed remodeling, causing a substantial increase in vessel diameter. In a collagen gel contraction assay, tTG determined the compaction of collagen by smooth muscle cells. Collectively, these data show that small artery remodeling associated with chronic vasoconstriction depends on tissue-type transglutaminase. This mechanism may reveal a novel therapeutic target for pathologies associated with inward remodeling of the resistance arterie