Identifying genes required for respiratory growth of fission yeast

We have used both auxotroph and prototroph versions of the latest deletion-mutant library to identify genes required for respiratory growth on solid glycerol medium in fission yeast. This data set complements and enhances our recent study on functional and regulatory aspects of energy metabolism by providing additional proteins that are involved in respiration. Most proteins identified in this mutant screen have not been implicated in respiration in budding yeast. We also provide a protocol to generate a prototrophic mutant library, and data on technical and biological reproducibility of colony-based high-throughput screens

The New Flexible Economy: Shaping Regional and Local Institutions for Global Competition

Series: IIR-Discussion Paper

1,5-Anhydroglucitol as a marker of maternal glycaemic control and predictor of neonatal birthweight in pregnancies complicated by type 1 diabetes mellitus

AIMS/HYPOTHESIS: Most pregnant women with type 1 diabetes mellitus achieve HbA(1c) targets; however, macrosomia remains prevalent and better pregnancy glycaemic markers are therefore needed. 1,5-Anhydroglucitol (1,5-AG) is a short-term marker of glycaemia, reflecting a period of 1 to 2 weeks. Its excretion rate depends on the renal glucose threshold and thus it is unclear whether it may be used in pregnant type 1 diabetes women. We evaluated 1,5-AG as a glycaemic marker and birthweight predictor in pregnant women with type 1 diabetes, and compared its performance with HbA(1c). METHODS: 1,5-AG and HbA(1c) were measured in 82 pregnant women with type 1 diabetes. In addition, 58 continuous glucose monitoring system (CGMS) records were available. Macrosomia was defined as birthweight >90th centile. The data were analysed with Pearson’s correlations, and linear and logistic regression models. Receiver operating characteristic (ROC) analysis was used to evaluate third trimester 1,5-AG as a predictor of macrosomia. RESULTS: Unlike HbA(1c), 1,5-AG strongly correlated with CGMS indices: the AUC above 7.8 mmol/l (r = −0.66; p < 0.001), average maximum glucose (r = −0.58; p < 0.001) and mean glucose (r = −0.54; p < 0.001). In the third trimester, 1,5-AG was the strongest predictor of macrosomia, with ROC AUC 0.81 (95% CI 0.70, 0.89). In contrast, HbA(1c) in the third trimester had a ROC AUC of 0.69 (95% CI 0.58, 0.81). The best discrimination was achieved when both markers were used jointly, yielding a ROC AUC of 0.84 (95% CI 0.76, 0.93). CONCLUSIONS/INTERPRETATION: In pregnant women with type 1 diabetes, 1,5-AG is a better glycaemic marker than HbA(1c), as assessed by CGMS. A decreased third trimester 1,5-AG level, either singly or with HbA(1c), is a strong predictor of macrosomia

3-Body Dynamics in a (1+1) Dimensional Relativistic Self-Gravitating System

The results of our study of the motion of a three particle, self-gravitating system in general relativistic lineal gravity is presented for an arbitrary ratio of the particle masses. We derive a canonical expression for the Hamiltonian of the system and discuss the numerical solution of the resulting equations of motion. This solution is compared to the corresponding non-relativistic and post-Newtonian approximation solutions so that the dynamics of the fully relativistic system can be interpretted as a correction to the one-dimensional Newtonian self-gravitating system. We find that the structure of the phase space of each of these systems yields a large variety of interesting dynamics that can be divided into three distinct regions: annulus, pretzel, and chaotic; the first two being regions of quasi-periodicity while the latter is a region of chaos. By changing the relative masses of the three particles we find that the relative sizes of these three phase space regions changes and that this deformation can be interpreted physically in terms of the gravitational interactions of the particles. Furthermore, we find that many of the interesting characteristics found in the case where all of the particles share the same mass also appears in our more general study. We find that there are additional regions of chaos in the unequal mass system which are not present in the equal mass case. We compare these results to those found in similar systems.Comment: latex, 26 pages, 17 figures, high quality figures available upon request; typos and grammar correcte