8 research outputs found
Characterization of minisatellites in Arabidopsis thaliana with sequence similarity to the human minisatellite core sequence.
No full textInternational audienceA strategy based on random PCR amplification was used to isolate new repetitive elements of Arabidopsis thaliana. One of the random PCR product analyzed by this approach contained a tandem repetitive minisatellite sequence composed of 33 bp repeated units. The genomic locus corresponding to this PCR product was isolated by screening a lambda genomic library. New related loci were also isolated from the genomic library by screening with a 14 mer oligonucleotide representing a region conserved among the different repeated units. Alignment of the consensus sequence for each minisatellite locus allowed the definition of an Arabidopsis thaliana core sequence that shows strong sequence similarities with the human core sequence and with the generalized recombination signal Chi of Escherichia coli. The minisatellites were tested for their ability to detect polymorphism, and their chromosomal position was established
Characterization of minisatellites in Arabidopsis thaliana with sequence similarity to the human minisatellite core sequence.
No full textA strategy based on random PCR amplification was used to isolate new repetitive elements of Arabidopsis thaliana. One of the random PCR product analyzed by this approach contained a tandem repetitive minisatellite sequence composed of 33 bp repeated units. The genomic locus corresponding to this PCR product was isolated by screening a lambda genomic library. New related loci were also isolated from the genomic library by screening with a 14 mer oligonucleotide representing a region conserved among the different repeated units. Alignment of the consensus sequence for each minisatellite locus allowed the definition of an Arabidopsis thaliana core sequence that shows strong sequence similarities with the human core sequence and with the generalized recombination signal Chi of Escherichia coli. The minisatellites were tested for their ability to detect polymorphism, and their chromosomal position was established
The CIC library: a large insert YAC library for genome mapping in Arabidopsis thaliana
No full textInternational audienc
The CIC YAC library : sizing of the clones and determination of clones carrying repeated DNA sequences
No full textJournal electronique. URL : http://nasc.nott.ac.uk:300International audienc
Reciprocal chromosome translocation associated with TDNA-insertion mutation in Arabidopsis: genetic and cytological analyses of consequences for gametophyte development and for construction of doubly mutant lines
No full textHigh-Level Production of MSH2 from Arabidopsis thaliana: A DNA Mismatch Repair System Key Subunit
No full text
