Kinetic parameters related to nitrogen uptake efficiency of pear trees (Pyrus communis).

Genetic improvement programs for pear trees in Brazil are characterized by rootstock (hypobiotic) selection mainly considering physiological attributes such as vigor, breakage of dormancy, propagation easiness, and sanitary characteristics, such as resistance to pests and diseases. However, kinetic parameters that determine nutrient uptake efficiency are not usually considered as, for example, nitrogen (N) in forms of NO3 − and NH4 +. The objective of this study was to evaluate the kinetic parameters related to N uptake in pear selection '54′ and '971′ as additional criteria for rootstock selection. The plants were acclimatized in a half-strength Hoagland solution and tested to assess the depletion of the internal reserves at 15 and 30 days, in CaSO4 solution to evaluate the depletion period during 65 h. The selection '971′ showed more significant NO3 − and NH4 + uptake efficiency, because presented higher Vmax values and Influx. The internal reserve depletion (IRD) period for the evaluated selections was 30 days in CaSO4 solution. The evaluation period of the depletion period to reach the Cmin for selections '971′ and '54′ was 64 and 65 h for NO3 − respectively, and 65 h for NH4 +. The selection '971′ had a higher affinity with the NO3 − ion when correlated with the physiological parameters of minimum fluorescence (Fo) and electron transport rate (ETRm)

Fertirrigação e lixiviação de nitrogênio em solo arenoso de vinhedo.

XV Congresso Latino-Americano de Viticultura e Enologia E XIII Congresso Brasileiro de Viticultura e Enologia. Bento Gonçalves-RS, 3 a 7 de Novembro de 2015

Procedimentos para avaliação de parâmetros cinéticos na absorção de nutrientes em videiras.

A maioria dos solos são carentes de nutrientes e exigem adubações. E as espécies e variedades cultivadas também podem apresentar baixa eficiência na absorção dos nutrientes, prejudicando o crescimento, produtividade e qualidade dos frutos. Como consequência, parte dos nutrientes aplicados via adubações pode ser desperdiçado, contribuindo com perdas e contaminações. Por isso, metodologias que contribuem para a seleção de genótipos mais eficientes na absorção de nutrientes, especialmente das formas de nutrientes mais móveis nos solos, são de extrema importância. Esta publicação apresenta uma metodologia inovadora para estimar os parâmetros cinéticos da absorção de nutrientes em videiras ainda no estágio de mudas, envolvendo a velocidade máxima de absorção, a constante de Michaelis-Menten, a concentração mínima e o influxo. A análise conjunta desses parâmetros cinéticos permite definir quais as variedades ou seleções avançadas possuem potencial para se adaptar aos solos com baixa fertilidade natural, ou mesmo, que podem receber menores doses de nutrientes, racionalizando o manejo da adubação e diminuindo os custos de produção

From fuzzy to annotated semantic web languages

The aim of this chapter is to present a detailed, selfcontained and comprehensive account of the state of the art in representing and reasoning with fuzzy knowledge in Semantic Web Languages such as triple languages RDF/RDFS, conceptual languages of the OWL 2 family and rule languages. We further show how one may generalise them to so-called annotation domains, that cover also e.g. temporal and provenance extensions

Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

Abstract The human polyomaviruses JC and BK (JCPyV and BKPyV) are ubiquitous, species-specific viruses that belong to the family Polyomaviridae. These viruses are known to be excreted in human urine, and they are potential indicators of human wastewater contamination. In order to assess the distribution of both JCPyV and BKPyV in urban water samples collected from a sewage treatment plant (STP) and from a canalized water stream of Porto Alegre, Brazil, two nested-PCR assays were optimized and applied to the samples collected. The amplicons obtained were submitted to sequencing, and the sequences were analyzed with sequences of human polyomaviruses previously deposited in GenBank. Twelve out of 30 water samples (40%) were JCPyV positive, whereas six samples (20%) were BKPyV positive. The sequencing results confirmed the presence of JCPyV subtypes 1 and 3, whereas only BKPyV Ia and Ib were found. This study shows for the first time the presence of human polyomaviruses in surface water and in samples collected in a sewage treatment plant in southern Brazil

Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

Abstract The human polyomaviruses JC and BK (JCPyV and BKPyV) are ubiquitous, species-specific viruses that belong to the family Polyomaviridae. These viruses are known to be excreted in human urine, and they are potential indicators of human wastewater contamination. In order to assess the distribution of both JCPyV and BKPyV in urban water samples collected from a sewage treatment plant (STP) and from a canalized water stream of Porto Alegre, Brazil, two nested-PCR assays were optimized and applied to the samples collected. The amplicons obtained were submitted to sequencing, and the sequences were analyzed with sequences of human polyomaviruses previously deposited in GenBank. Twelve out of 30 water samples (40%) were JCPyV positive, whereas six samples (20%) were BKPyV positive. The sequencing results confirmed the presence of JCPyV subtypes 1 and 3, whereas only BKPyV Ia and Ib were found. This study shows for the first time the presence of human polyomaviruses in surface water and in samples collected in a sewage treatment plant in southern Brazil

A 5-year multicentre randomized controlled trial comparing personalized, frozen and fresh blastocyst transfer in IVF

RESEARCH QUESTION:Does clinical performance of personalized embryo transfer (PET) guided by endometrial receptivity analysis (ERA) differ from frozen embryo transfer (FET) or fresh embryo transfer in infertile patients undergoing IVF? DESIGN:Multicentre, open-label randomized controlled trial; 458 patients aged 37 years or younger undergoing IVF with blastocyst transfer at first appointment were randomized to PET guided by ERA, FET or fresh embryo transfer in 16 reproductive clinics. RESULTS:Clinical outcomes by intention-to-treat analysis were comparable, but cumulative pregnancy rate was significantly higher in the PET (93.6%) compared with FET (79.7%) (P = 0.0005) and fresh embryo transfer groups (80.7%) (P = 0.0013). Analysis per protocol demonstrates that live birth rates at first embryo transfer were 56.2% in PET versus 42.4% in FET (P = 0.09), and 45.7% in fresh embryo transfer groups (P = 0.17). Cumulative live birth rates after 12 months were 71.2% in PET versus 55.4% in FET (P = 0.04), and 48.9% in fresh embryo transfer (P = 0.003). Pregnancy rates at the first embryo transfer in PET, FET and fresh embryo transfer arms were 72.5% versus 54.3% (P = 0.01) and 58.5% (P = 0.05), respectively. Implantation rates at first embryo transfer were 57.3% versus 43.2% (P = 0.03), and 38.6% (P = 0.004), respectively. Obstetrical outcomes, type of delivery and neonatal outcomes were similar in all groups. CONCLUSIONS:Despite 50% of patients dropping out compared with 30% initially planned, per protocol analysis demonstrates statistically significant improvement in pregnancy, implantation and cumulative live birth rates in PET compared with FET and fresh embryo transfer arms, indicating the potential utility of PET guided by the ERA test at the first appointment.Carlos Simón, Carlos Gómez, Sergio Cabanillas, Iavor Vladimirov, Gemma Castillón ... Bernardus Mol ... et al