41 research outputs found
Antimicrobial Peptide Evolution in the Asiatic Honey Bee Apis cerana
The Asiatic honeybee, Apis cerana Fabricius, is an important honeybee species in Asian countries. It is still found in the wild, but is also one of the few bee species that can be domesticated. It has acquired some genetic advantages and significantly different biological characteristics compared with other Apis species. However, it has been less studied, and over the past two decades, has become a threatened species in China. We designed primers for the sequences of the four antimicrobial peptide cDNA gene families (abaecin, defensin, apidaecin, and hymenoptaecin) of the Western honeybee, Apis mellifera L. and identified all the antimicrobial peptide cDNA genes in the Asiatic honeybee for the first time. All the sequences were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). In all, 29 different defensin cDNA genes coding 7 different defensin peptides, 11 different abaecin cDNA genes coding 2 different abaecin peptides, 13 different apidaecin cDNA genes coding 4 apidaecin peptides and 34 different hymenoptaecin cDNA genes coding 13 different hymenoptaecin peptides were cloned and identified from the Asiatic honeybee adult workers. Detailed comparison of these four antimicrobial peptide gene families with those of the Western honeybee revealed that there are many similarities in the quantity and amino acid components of peptides in the abaecin, defensin and apidaecin families, while many more hymenoptaecin peptides are found in the Asiatic honeybee than those in the Western honeybee (13 versus 1). The results indicated that the Asiatic honeybee adult generated more variable antimicrobial peptides, especially hymenoptaecin peptides than the Western honeybee when stimulated by pathogens or injury. This suggests that, compared to the Western honeybee that has a longer history of domestication, selection on the Asiatic honeybee has favored the generation of more variable antimicrobial peptides as protection against pathogens
New approach to the study of division of labour in the honeybee colony (Apis mellifera L)
Newly discovered features of the updated sequence of royal jelly protein RJP571; longer repetitive region on C-terminus and homology to Drosophila melanogaster yellow protein
Characterization by CDNA cloning of a highly abundant messenger-RNA from human ovarian granulosa-cells.
New approach to the study of division of labour in the honeybee colony (Apis mellifera L)
Using Northern analysis we determined differences in the transcription level of 4 mRNAs (coding for larval food proteins) in the heads of sister worker bees, which were either foragers or nurses rearing the larvae of workers, drones and queens. The ratio of the isolated total RNAs from the heads of these bee samples was estimated as 1:2:1.9:3.6, respectively