Further characterization of antigen-dependent migration inhibition factor in mice.

Antigen-dependent MIF was produced in inbred mice. Lymph node cells from mice sensitized to tubercle bacilli were incubated with small quantities of antigen. The supernatant contained antigen-dependent MIF and its activity when tested on mouse and rabbit spleen was minimal unless PPD was added to the supernatant. The production of the antigen-dependent MIF was T cell-dependent, as shown by the use of anti-theta serum. Its molecular weight was in the range of 50--100,000 and it was concluded that the factor is different from conventional antibodies or antigen-antibody complexes

Chemical properties of antigen-dependent migration inhibition factor in mice.

Antigen-dependent migration inhibition factor produced in cultures of mouse lymph node lymphocytes was further characterized. It was found to bind to Concanavalin A and could be eluted by the appropriate sugar. The pI of the activity was found to be between 6-4 and 6-5 by isoelectric focusing