24 research outputs found

    3' non-templated 'A' addition by Taq DNA polymerase: An advantage in the construction of single and double mutants

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    The 3'-end non-templated 'A' addition by Tag DNA polymerase has been described as a disadvantage in the generation of site-specific mutants as this 'A' leads to a unplanned second mutation, Here we demonstrate the utility of this 3' non-templated 'A' addition in the simultaneous construction of single and double mutants of serine hydroxymethyltransferas

    3'non-templated 'A' addition by Taq DNA polymerase: an advantage in the construction of single and double mutants

    No full text
    The 3'-end non-templated 'A' addition by Tag DNA polymerase has been described as a disadvantage in the generation of site-specific mutants as this 'A' leads to a unplanned second mutation, Here we demonstrate the utility of this 3′ non-templated 'A' addition in the simultaneous construction of single and double mutants of serine hydroxymethyltransferase

    Alirocumab and cardiovascular outcomes after acute coronary syndrome

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    BACKGROUN

    Alirocumab and Cardiovascular Outcomes after Acute Coronary Syndrome

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    BACKGROUN

    Effects of alirocumab on types of myocardial infarction: insights from the ODYSSEY OUTCOMES trial

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    Effects of alirocumab on types of myocardial infarction: insights from the ODYSSEY OUTCOMES trial

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