12 research outputs found
Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella
Polymorphism at the dnaK locus of Brucella species and identification of a Brucella melitensis species-specific marker
Classification of Brucella spp. from marine mammals by DNA polymorphism at the omp2 locus.
Classification of Brucella spp. from marine mammals by DNA polymorphism at the omp2 locus.
Simultaneous expression of smooth and rough phase properties related to lipopolysaccharide in a strain of Brucella melitensis
Molecular Typing of Brucella With Cloned Dna Probes
Brucella constitutes a single genomic species (B. melitensis); however, for epidemiological studies, methods are needed for discriminating strains within this genomic species. DNA samples from 112 Brucella strains were cleaved by restriction endonucleases and the fragments separated by agarose gel electrophoresis and transferred to nylon membranes. When the DNA fragments on the membranes were probed with P-32-labelled 16 + 23 S rRNA from Escherichia coli, a single rRNA gene restriction pattern was obtained after cleavage with all endonucleases tested (HindIII, EcoRI, SmaI, and XhoI) except BamHI. This indicated high genomic homogeneity within the single Brucella species.
Of 30 probes consisting of random Brucella DNA fragments cloned into lambda-EMBL3, 20 yielded a single BamHI restriction pattern per probe when applied to 112 Brucella DNA tested. However, 7 probes yielded 3 to 12 different patterns among DNA tested. These patterns more-or-less correlated with the classification of strains into biogroups (Melitensis, Abortus, Suis, Neotomae, Ovis and Canis) and biovars (18 biovars represented). Probe A was capable of separating biogroup Melitensis from the other biogroups. Probe C separated the set of biogroups Melitensis-Abortus-Ovis from the other biogroups. By reference to the patterns obtained using 1 to 7 probes, the most frequently occurring biovars (Melitensis 1, Melitensis 3, Abortus 1, Abortus 3, Suis 2 and Ovis) could be distinguished from each other. Eight biovars showed more than one pattern with 1 to 7 probes.
The proposed typing system should be useful for epidemiological subtyping and does not pose safety problems once the DNA has been extracted
Phenotypic and molecular characterization of a Brucella strain isolated from a minke whale (Balaenoptera acutorostrata)
Evaluation of a Multiplex PCR Assay (Bruce-ladder) for Molecular Typing of All Brucella Species, Including the Vaccine Strainsâż â
An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains
Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains
An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains