24 research outputs found
Performance of a light applicator for photodynamic therapy in the oral cavity: calculations and measurements
Provision of water by halite deliquescence for Nostoc commune biofilms under Mars relevant surface conditions
Motivated by findings of new mineral related water sources for organisms under extremely dry conditions on Earth we studied in an interdisciplinary approach the water sorption behaviour of halite, soil component and terrestrial Nostoc commune biofilm under Mars relevant environmental conditions. Physicochemical methods served for the determination of water sorption equilibrium data and survival of heterotrophic bacteria in biofilm samples with different water contents was assured by recultivation. Deliquescence of halite provides liquid water at temperatures <273 K and may serve as water source on Mars during themorning stabilized by the CO2 atmosphere for a few hours. The protecting biofilmof N. commune is rather hygroscopic and tends to store water at lower humidity values. Survival tests showed that a large proportion of the Alphaproteobacteria dominated microbiota associated to N. commune is very desiccation tolerant and water uptake from saturated NaCl solutions (either by direct uptake of brine or adsorption of humidity) did not enhance recultivability in long-time desiccated samples. Still, a minor part can grow under highly saline conditions.However, the salinity level, although unfavourable for the host organism, might be for parts of the heterotrophic microbiota no serious hindrance for growing in salty Mars-like environments
Desiccation tolerance of iron bacteria biofilms on Mars regolith simulants
Iron oxidizing bacteria play an important role in the geological redox cycling of iron on earth. The redox change
between Fe(II) and Fe(III) can be used for biological energy production [1]. Therefore iron oxidation in the iron
rich martian soils may be or may have been microbially mediated. The microbial conversion of iron is considered
to be an ancient form of metabolism [2], so it might have evolved on Mars as well. However, to exist in recent
martian soils, bacteria must be able to endure dry and cold conditions.
Neutrophilic iron oxidizers can be found in various iron rich aquatic environments, where they lead to the
precipitation of insoluble ferric hydroxides. Some of these environments fall temporarily dry, what could have led
to an adaptation to desiccation by bacteria, existing there. One strategy of iron bacteria to endure drought stress
might be the formation of biofilms by excreting Extracellular Polymeric Substances (EPS). The deposition of iron
hydroxides could enable them to endure dry conditions as well.
For our experiments, neutrophilic iron oxidizing bacteria have been isolated from a creek in Bad Salzhausen/Hesse
and temporarily drying out pools in Tierra del Fuego. Strains from aquatic environments in the national park
"Unteres Odertal" and from water wells in Berlin/Brandenburg are included in the tests as well.
In desiccation experiments, the capability of iron bacteria to tolerate dry conditions are investigated. The aim of
our first experiment is the adaptation to dry conditions. Biofilms of 15 strains are grown on ceramic beads in liquid
medium containing complexed Fe(II), established biofilms contain Fe(III) precipitates. The cultures are desiccated
in a sterile airflow until the weight of the cultures remained constant. After a desiccation period of 9 h up to 7 d,
the beads are transferred to fresh liquid medium.
Adapted strains are used in further desiccation experiments, where biofilms are grown on two martian regolith
simulants. These mineral mixtures were developed and produced by the Naturkundemuseum Berlin according to
recent data of Mars research missions [3, 4, 5, 6, 7]. The minerals are attached to object slides with potassium
silicate and biofilms are grown on the mineral surface. The biofilms are quantified by cell counting and the
structure is evaluated by epifluorescence microscopy. After desiccation in a sterile airflow, the survival of cells is
determined by fluorescence staining
Water retention of selected microorganisms and Martian soil simulants under close to Martian environmental conditions
Based on the latest knowledge about microorganisms resistant towards extreme conditions on Earth and results of new complex models on the development of the Martian atmosphere we quantitatively examined the water-bearing properties of selected extremophiles and simulated Martian regolith components and their interaction with water vapor under close to Martian environmental conditions. Three different species of microorganisms have been chosen and prepared for our study: Deinococcus geothermalis, Leptothrix sp. OT_B_406, and Xanthoria elegans. Further, two mineral mixtures representing the early and the late Martian surface as well as montmorillonite as a single component of phyllosilicatic minerals, typical for the Noachian period on Mars, were selected. The thermal mass loss of the minerals and bacteria-samples was measured by thermoanalysis. The hydration and dehydration properties were determined under close to Martian environmental conditions by sorption isotherm measurements using a McBain-Bakr quartz spring balance. It was possible to determine the total water content of the materials as well as the reversibly bound water fraction as function of the atmospheres humidity by means of these methods. Our results are important for the evaluation of future space mission outcomes including astrobiological aspects and can support the modeling of the atmosphere/surface interaction by showing the influence on the water inventory of the upper most layer of the Martian surface
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Cisplatinum and interstitial laser therapy for advanced head and neck cancer: A preclinical study
Genetic cause and prevalence of hydroxyprolinemia.
BACKGROUND: Hydroxyprolinemia is an inborn error of amino acid degradation that is considered a non-disease. Known for more than 50 years, its genetic cause and prevalence have remained unclear. In MS/MS newborn screening, the mass spectrum of hydroxyproline cannot be differentiated from isoleucine and leucine causing false positive newborn screening test results for maple syrup urine disease (MSUD). METHODS: We studied two siblings with hydroxyprolinemia via exome sequencing and confirmed the candidate gene in five further individuals with hydroxyprolinemia, who were all characterized biochemically and clinically. The prevalence was calculated based on the number of individuals with hydroxyprolinemia detected via MS/MS newborn screening at our centre from 2003 to 2014. RESULTS: In six cases, we identified homozygous or compound heterozygous mutations in PRODH2 as the underlying genetic cause of hydroxyprolinemia. One individual was heterozygous for a deletion in PRODH2 and had an intermittent biochemical phenotype with partial normalization of hydroxyproline concentrations. In one further individual with persistent hydroxyprolinemia no mutation in PRODH2 was found, raising the possibility of another defect of hydroxyproline degradation yet to be identified as the underlying cause of hydroxyprolinemia. Plasma hydroxyproline concentrations were clearly elevated in all individuals with biallelic mutations in PRODH2. All studied individuals remained asymptomatic, giving further evidence that hydroxyprolinemia is a benign condition. The estimated prevalence of hydroxyprolinemia in Germany is about one in 47,300 newborns. CONCLUSION: Our results establish mutations in PRODH2 as a cause of human hydroxyprolinemia via impaired dehydrogenation of hydroxyproline to delta1-pyroline-3-hydroxy-5-carboxylic acid, and we suggest PRODH2 be renamed HYPDH. Hydroxyprolinemia is an autosomal-recessively inherited benign condition. It is a frequent cause of false positive screening results for MSUD, the prevalence being about 2.5 times higher than that of MSUD