Scientific CCD technology at JPL

Charge-coupled devices (CCD's) were recognized for their potential as an imaging technology almost immediately following their conception in 1970. Twenty years later, they are firmly established as the technology of choice for visible imaging. While consumer applications of CCD's, especially the emerging home video camera market, dominated manufacturing activity, the scientific market for CCD imagers has become significant. Activity of the Jet Propulsion Laboratory and its industrial partners in the area of CCD imagers for space scientific instruments is described. Requirements for scientific imagers are significantly different from those needed for home video cameras, and are described. An imager for an instrument on the CRAF/Cassini mission is described in detail to highlight achieved levels of performance

Collisional dissipation of Alfvén waves in a partially ionised solar chromosphere

Certain regions of the solar atmosphere are at sufficiently low temperatures to be only partially ionised. The lower chromosphere contains neutral atoms, the existence of which greatly increases the efficiency of the damping of waves due to collisional friction momentum transfer. More specifically the Cowling conductivity can be up to 12 orders of magnitude smaller than the Spitzer value, so that the main damping mechanism in this region is due to the collisions between neutrals and positive ions (Khodachenko et al. 2004, A&A, 422, 1073). Using values for the gas density and temperature as functions of height taken from the VAL C model of the quiet Sun (Vernazza et al. 1981, ApJS, 45, 635), an estimate is made for the dependance of the Cowling conductivity on height and strength of magnetic field. Using both analytic and numerical approaches the passage of Alfvén waves over a wide spectrum through this partially ionised region is investigated. Estimates of the efficiency of this region in the damping of Alfvén waves are made and compared for both approaches. We find that Alfvén waves with frequencies above 0.6 Hz are completely damped and frequencies below 0.01 Hz unaffected

Asynchronous Corner Tracking Algorithm based on Lifetime of Events for DAVIS Cameras

Event cameras, i.e., the Dynamic and Active-pixel Vision Sensor (DAVIS) ones, capture the intensity changes in the scene and generates a stream of events in an asynchronous fashion. The output rate of such cameras can reach up to 10 million events per second in high dynamic environments. DAVIS cameras use novel vision sensors that mimic human eyes. Their attractive attributes, such as high output rate, High Dynamic Range (HDR), and high pixel bandwidth, make them an ideal solution for applications that require high-frequency tracking. Moreover, applications that operate in challenging lighting scenarios can exploit the high HDR of event cameras, i.e., 140 dB compared to 60 dB of traditional cameras. In this paper, a novel asynchronous corner tracking method is proposed that uses both events and intensity images captured by a DAVIS camera. The Harris algorithm is used to extract features, i.e., frame-corners from keyframes, i.e., intensity images. Afterward, a matching algorithm is used to extract event-corners from the stream of events. Events are solely used to perform asynchronous tracking until the next keyframe is captured. Neighboring events, within a window size of 5x5 pixels around the event-corner, are used to calculate the velocity and direction of extracted event-corners by fitting the 2D planar using a randomized Hough transform algorithm. Experimental evaluation showed that our approach is able to update the location of the extracted corners up to 100 times during the blind time of traditional cameras, i.e., between two consecutive intensity images.Comment: Accepted to 15th International Symposium on Visual Computing (ISVC2020

An Interdigitated Pixel PIN Detector for Energetic Particle Spectroscopy in Space

We describe a new two-dimensional position-sensitive detector, now under development, for use in space-borne energetic particle spectrometers. The novel feature of this device is the use of interdigitated pixels to provide both dimensions of position information from a single side of the detector, while a measurement of the energy deposition is derived from the opposite side. An advantage of this approach is that significant reductions in the complexity, power, and weight of the associated read-out electronics can be realized without sacrificing position or energy resolution

An integrated space physics instrument (ISPI) for Solar Probe

Instruments for the Solar Probe mission must be designed not only to address the unique scientific measurement requirements, but must be compatible with the modest resource dollars as well as tight constraints on mass and power. Another unique aspect of the Solar Probe mission is its constraint on telemetry and the fact that the prime science is conducted in a single flyby. The instrument system must be optimized to take advantage of the telemetry and observing time available. JPL, together with industry and university partners, is designing an Integrated Space Physics Instrument (ISPI) which will measure magnetic fields, plasma waves, thermal plasma, energetic particles, dust, and perform EUV/visible and coronal imaging for the Solar Probe mission. ISPI uses a new architecture and incorporates technology which not only eliminates unnecessary duplication of function, but allows sensors to share data and optimize science. The current ISPI design goal (for a flight package) is a 5 kilogram/10 watt payload. © 1997 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87393/2/131_1.pd

A systematic review and meta-synthesis of the impact of low back pain on people's lives

Copyright @ 2014 Froud et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.Background - Low back pain (LBP) is a common and costly problem that many interpret within a biopsychosocial model. There is renewed concern that core-sets of outcome measures do not capture what is important. To inform debate about the coverage of back pain outcome measure core-sets, and to suggest areas worthy of exploration within healthcare consultations, we have synthesised the qualitative literature on the impact of low back pain on people’s lives. Methods - Two reviewers searched CINAHL, Embase, PsycINFO, PEDro, and Medline, identifying qualitative studies of people’s experiences of non-specific LBP. Abstracted data were thematic coded and synthesised using a meta-ethnographic, and a meta-narrative approach. Results - We included 49 papers describing 42 studies. Patients are concerned with engagement in meaningful activities; but they also want to be believed and have their experiences and identity, as someone ‘doing battle’ with pain, validated. Patients seek diagnosis, treatment, and cure, but also reassurance of the absence of pathology. Some struggle to meet social expectations and obligations. When these are achieved, the credibility of their pain/disability claims can be jeopardised. Others withdraw, fearful of disapproval, or unable or unwilling to accommodate social demands. Patients generally seek to regain their pre-pain levels of health, and physical and emotional stability. After time, this can be perceived to become unrealistic and some adjust their expectations accordingly. Conclusions - The social component of the biopsychosocial model is not well represented in current core-sets of outcome measures. Clinicians should appreciate that the broader impact of low back pain includes social factors; this may be crucial to improving patients’ experiences of health care. Researchers should consider social factors to help develop a portfolio of more relevant outcome measures.Arthritis Research U

Ras C AAX Peptidomimetic FTI-277 Selectively Blocks Oncogenic Ras Signaling by Inducing Cytoplasmic Accumulation of Inactive Ras-Raf Complexes

Ras-induced malignant transformation requires Ras farnesylation, a lipid posttranslational modification catalyzed by farnesyltransferase (FTase). Inhibitors of this enzyme have been shown to block Ras-dependent transformation, but the mechanism by which this occurs remains largely unknown. We have designed FTI-276, a peptide mimetic of the COOH-terminal Cys-Val-Ile-Met of K-Ras4B that inhibited potently FTase in vitro (IC50 = 500 pM) and was highly selective for FTase over geranylgeranyltransferase I (GGTase I) (IC50 = 50 nM). FTI-277, the methyl ester derivative of FTI-276, was extremely potent (IC50 = 100 nM) at inhibiting H-Ras, but not the geranylgeranylated Rap1A processing in whole cells. Treatment of H-Ras oncogene-transformed NIH 3T3 cells with FTI-277 blocked recruitment to the plasma membrane and subsequent activation of the serine/threonine kinase c-Raf-1 in cells transformed by farnesylated Ras (H-RasF), but not geranylgeranylated, Ras (H-RasGG). FTI-277 induced accumulation of cytoplasmic non-farnesylated H-Ras that was able to bind Raf and form cytoplasmic Ras/Raf complexes in which Raf kinase was not activated. Furthermore, FTI-277 blocked constitutive activation of mitogen-activated protein kinase (MAPK) in H-RasF, but not H-RasGG, or Raf-transformed cells. FTI-277 also inhibited oncogenic K-Ras4B processing and constitutive activation of MAPK, but the concentrations required were 100-fold higher than those needed for H-Ras inhibition. The results demonstrate that FTI-277 blocks Ras oncogenic signaling by accumulating inactive Ras/Raf complexes in the cytoplasm, hence preventing constitutive activation of the MAPK cascade

A Reduction in Ribonucleotide Reductase Activity Slows Down the Chromosome Replication Fork but Does Not Change Its Localization

BACKGROUND:It has been proposed that the enzymes of nucleotide biosynthesis may be compartmentalized or concentrated in a structure affecting the organization of newly replicated DNA. Here we have investigated the effect of changes in ribonucleotide reductase (RNR) activity on chromosome replication and organization of replication forks in Escherichia coli. METHODOLOGY/PRINCIPAL FINDINGS:Reduced concentrations of deoxyribonucleotides (dNTPs) obtained by reducing the activity of wild type RNR by treatment with hydroxyurea or by mutation, resulted in a lengthening of the replication period. The replication fork speed was found to be gradually reduced proportionately to moderate reductions in nucleotide availability. Cells with highly extended C periods showed a "delay" in cell division i.e. had a higher cell mass. Visualization of SeqA structures by immunofluorescence indicated no change in organization of the new DNA upon moderate limitation of RNR activity. Severe nucleotide limitation led to replication fork stalling and reversal. Well defined SeqA structures were not found in situations of extensive replication fork repair. In cells with stalled forks obtained by UV irradiation, considerable DNA compaction was observed, possibly indicating a reorganization of the DNA into a "repair structure" during the initial phase of the SOS response. CONCLUSION/SIGNIFICANCE:The results indicate that the replication fork is slowed down in a controlled manner during moderate nucleotide depletion and that a change in the activity of RNR does not lead to a change in the organization of newly replicated DNA. Control of cell division but not control of initiation was affected by the changes in replication elongation