1,474 research outputs found
Human neuronal stargazin-like proteins, γ_2, γ_3 and γ_4; an investigation of their specific localization in human brain and their influence on Ca_V2.1 voltage-dependent calcium channels expressed in Xenopus oocytes
Background: Stargazin (γ2) and the closely related γ3, and γ4 transmembrane proteins are part of a family of proteins that may act as both neuronal voltage-dependent calcium channel (VDCC) γ subunits and transmembrane α-amino-3-hydroxy-5-methyl-4-isoxazoleproponinc (AMPA) receptor regulatory proteins (TARPs). In this investigation, we examined the distribution patterns of the stargazin-like proteins γ2, γ3, and γ4 in the human central nervous system (CNS). In addition, we investigated whether human γ2 or γ4 could modulate the electrophysiological properties of a neuronal VDCC complex transiently expressed in Xenopus oocytes.
Results: The mRNA encoding human γ2 is highly expressed in cerebellum, cerebral cortex, hippocampus and thalamus, whereas γ3 is abundant in cerebral cortex and amygdala and γ4 in the basal ganglia. Immunohistochemical analysis of the cerebellum determined that both γ2 and γ4 are present in the molecular layer, particularly in Purkinje cell bodies and dendrites, but have an inverse expression pattern to one another in the dentate cerebellar nucleus. They are also detected in the interneurons of the granule cell layer though only γ2 is clearly detected in granule cells. The hippocampus stains for γ2 and γ4 throughout the layers of the every CA region and the dentate gyrus, whilst γ3 appears to be localized particularly to the pyramidal and granule cell bodies. When co-expressed in Xenopus oocytes with a CaV2.1/β4 VDCC complex, either in the absence or presence of an α2δ2 subunit, neither γ2 nor γ4 significantly modulated the VDCC peak current amplitude, voltage-dependence of activation or voltage-dependence of steady-state inactivation.
Conclusion: The human γ2, γ3 and γ4 stargazin-like proteins are detected only in the CNS and display differential distributions among brain regions and several cell types in found in the cerebellum and hippocampus. These distribution patterns closely resemble those reported by other laboratories for the rodent orthologues of each protein. Whilst the fact that neither γ2 nor γ4 modulated the properties of a VDCC complex with which they could associate in vivo in Purkinje cells adds weight to the hypothesis that the principal role of these proteins is not as auxiliary subunits of VDCCs, it does not exclude the possibility that they play another role in VDCC function
The use of imaging systems to monitor shoreline dynamics
The development of imaging systems is nowadays established as one of the most powerful and reliable tools for monitoring beach morphodynamics. Two different techniques for shoreline detection are presented here and, in one case, applied to the study of beach width oscillations on a sandy beach (Pauanui Beach, New Zealand). Results indicate that images can provide datasets whose length and sample interval are accurate enough to resolve inter-annual and seasonal oscillations, and long-term trends. Similarly, imaging systems can be extremely useful in determining the statistics of rip current occurrence. Further improvements in accuracy and reliability are expected with the recent introduction of digital systems
HST Survey of Clusters in Nearby Galaxies. II. Statistical Analysis of Cluster Populations
We present a statistical system that can be used in the study of cluster
populations. The basis of our approach is the construction of synthetic cluster
color-magnitude-radius diagrams (CMRDs), which we compare with the observed
data using a maximum likelihood calculation. This approach permits a relatively
easy incorporation of incompleteness (a function of not only magnitude and
color, but also radius), photometry errors and biases, and a variety of other
complex effects into the calculation, instead of the more common procedure of
attempting to correct for those effects.
We then apply this procedure to our NGC 3627 data from Paper I. We find that
we are able to successfully model the observed CMRD and constrain a number of
parameters of the cluster population. We measure a power law mass function
slope of alpha = -1.50 +/- 0.07, and a distribution of core radii centered at
r_c = 1.53 +/- 0.15 pc. Although the extinction distribution is less
constrained, we measured a value for the mean extinction consistent with that
determined in Paper I from the Cepheids.Comment: 21 pages, 3 figures accepted for publication by A
The Asymptotic Giant Branch and the Tip of the Red Giant Branch as Probes of Star Formation History: The Nearby Dwarf Irregular Galaxy KKH 98
We investigate the utility of the asymptotic giant branch (AGB) and the red
giant branch (RGB) as probes of the star formation history (SFH) of the nearby
(D=2.5 Mpc) dwarf irregular galaxy, KKH 98. Near-infrared (IR) Keck Laser Guide
Star Adaptive Optics (AO) images resolve 592 IR bright stars reaching over 1
magnitude below the Tip of the Red Giant Branch. Significantly deeper optical
(F475W and F814W) Hubble Space Telescope images of the same field contain over
2500 stars, reaching to the Red Clump and the Main Sequence turn-off for 0.5
Gyr old populations. Compared to the optical color magnitude diagram (CMD), the
near-IR CMD shows significantly tighter AGB sequences, providing a good probe
of the intermediate age (0.5 - 5 Gyr) populations. We match observed CMDs with
stellar evolution models to recover the SFH of KKH 98. On average, the galaxy
has experienced relatively constant low-level star formation (5 x 10^-4 Mo
yr^-1) for much of cosmic time. Except for the youngest main sequence
populations (age < 0.1 Gyr), which are typically fainter than the AO data flux
limit, the SFH estimated from the the 592 IR bright stars is a reasonable match
to that derived from the much larger optical data set. Differences between the
optical and IR derived SFHs for 0.1 - 1 Gyr populations suggest that current
stellar evolution models may be over-producing the AGB by as much as a factor
of three in this galaxy. At the depth of the AO data, the IR luminous stars are
not crowded. Therefore these techniques can potentially be used to determine
the stellar populations of galaxies at significantly further distances.Comment: 15 pages, 14 figs, accepted for publication in Ap
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