Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach

Chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is composed of two different subunits, GapA and GapB. cDNA clones containing the entire coding sequences of the cytosolic precursors for GapA from pea and for GapB from pea and spinach have been identified, sequenced and the derived amino acid sequences have been compared to the corresponding sequences from tobacco, maize and mustard. These comparisons show that GapB differs from GapA in about 20% of its amino acid residues and by the presence of a flexible and negatively charged C-terminal extension, possibly responsible for the observed association of the enzyme with chloroplast envelopes in vitro. This C-terminal extension (29 or 30 residues) may be susceptible to proteolytic cleavage thereby leading to a conversion of chloroplast GAPDH isoenzyme I into isoenzyme II. Evolutionary rate comparisons at the amino acid sequence level show that chloroplast GapA and GapB evolve roughly two-fold slower than their cytosolic counterpart GapC. GapA and GapB transit peptides evolve about 10 times faster than the corresponding mature subunits. They are relatively long (68 and 83 residues for pea GapA and spinach GapB respectively) and share a similar amino acid framework with other chloroplast transit peptides

An adjunction formula for the Emerton-Jacquet functor

The Emerton–Jacquet functor is a tool for studying locally analytic representations of p-adic Lie groups. It provides a way to access the theory of p-adic automorphic forms. Here we give an adjunction formula for the Emerton–Jacquet functor, relating it directly to locally analytic inductions, under a strict hypothesis that we call non-critical. We also further study the relationship to socles of principal series in the non-critical setting

Screening and identification of seed-specific genes using digital differential display tools combined with microarray data from common wheat

<p>Abstract</p> <p>Background</p> <p>Wheat is one of the most important cereal crops for human beings, with seeds being the tissue of highly economic value. Various morphogenetic and metabolic processes are exclusively associated with seed maturation. The goal of this study was to screen and identify genes specifically expressed in the developing seed of wheat with an integrative utilization of digital differential display (DDD) and available online microarray databases.</p> <p>Results</p> <p>A total of 201 unigenes were identified as the results of DDD screening and microarray database searching. The expressions of 6 of these were shown to be seed-specific by qRT-PCR analysis. Further GO enrichment analysis indicated that seed-specific genes were mainly associated with defense response, response to stress, multi-organism process, pathogenesis, extracellular region, nutrient reservoir activity, enzyme inhibitor activity, antioxidant activity and oxidoreductase activity. A comparison of this set of genes with the rice (<it>Oryza sativa</it>) genome was also performed and approximately three-fifths of them have rice counterparts. Between the counterparts, around 63% showed similar expression patterns according to the microarray data.</p> <p>Conclusions</p> <p>In conclusion, the DDD screening combined with microarray data analysis is an effective strategy for the identification of seed-specific expressed genes in wheat. These seed-specific genes screened during this study will provide valuable information for further studies about the functions of these genes in wheat.</p

Effect of Atmosphere in a Foundry Mould on Casting Surface Quality

Changes of gas pressure in the moulding sand in the zone adjacent to mould cavity were analysed during pouring of cast iron. No significant effect of pressure on the surface quality of castings was observed. In the second series of tests, the concentration of hydrogen in the gas atmosphere was measured. It has been found that the value of this concentration depends on metal composition and is particularly high in cast iron containing magnesium. This is due to the reduction of water vapour with the element that has high affinity to oxygen. The presence of hydrogen causes the formation of gas-induced defects on the casting surface

Atmosfera gazowa występująca podczas wykonywania odlewów w technologii pełnej formy

A test mold was designed to investigate the full mold process. Pressure changes in a gap developing between the pattern made of foamed polystyrene and the raising column of liquid alloy were determined. Studies were conducted pouring molds with cast iron and with AK11 silumin. It was found that pressure is significantly higher in the case of cast iron, due to a higher rate of pattern decomposition and more advanced dissociation of hydrocarbons. The increase in pressure was observed to have no significant effect on changes in the pouring rate as it was compensated by higher metallostatic pressure. The increase of pressure occurs immediately after the start of pouring, it reaches a maximum and then stabilizes or decreases smoothly as a result of the drop in metallostatic pressure. The temperature of pouring has a significant effect on the decomposition rate of hydrocarbons evolving from the metal. During pouring of cast iron, the presence of hydrogen was revealed. It was particularly evident across the gas-permeable coating, where the concentration has exceeded 40%. Hydrogen precipitation was accompanied by an evolution on the casting surface of considerable amounts of pyrolytic carbon. It was found that gases forming in the gap were totally free from oxygen.Zaprojektowano próbną formę do badania procesu full mold. Określono zmiany ciśnienia w szczelinie powstającej pomiędzy styrodurowym modelem a podnoszącym się ciekłym stopem. Badania prowadzono przy zalewaniu form żeliwem oraz siluminem AK11. Stwierdzono, że ciśnienie to jest znacznie wyższe w przypadku żeliwa, co wynika z większej szybkości rozpadu modelu i dalej posuniętej dysocjacji węglowodorów. Wzrost ciśnienia nie powoduje jednak istotnej zmiany szybkości zalewania, ponieważ jest on kompensowany wyższym ciśnieniem metalostatycznym. Wzrost ciśnienia następuje bezpośrednio po rozpoczęciu zalewania, osiąga ono maksimum poczem stabilizuje się lub łagodnie maleje w wyniku zmniejszania ciśnienia metalostatycznego. Temperatura zalewania ma istotny wpływ na stopień rozpadu, wydzielających się z modelu węglowodorów. Przy zalewaniu żeliwa stwierdzono obecność wodoru. Jest ona widoczna zwłaszcza po drugiej stronie powłoki gazoprzepuszczalnej gdzie stężenie sięga powyżej 40%. Wydzielaniu wodoru towarzyszy wydzielanie się na powierzchni odlewu, znacznych ilości pyrolitycznego węgla. Stwierdzono, że gazy tworzące szczelinę są całkowicie wolne od tlenu

Studies of Gas Atmosphere Near the Metal-mould Interface During Casting and Solidification of Ductile Iron

In sand moulds, at a distance of 3 mm from the metal- mould interface, the sensors of temperature, and of oxygen and hydrogen content were installed. Temperature and the evolution of partial gas pressure have been analysed in moulds bonded with bentonite with or without the addition of seacoal, water glass or furan resin. Moulds were poured with ductile iron. For comparison, also tests with the grey iron have been executed. It was found that the gas atmosphere near the interface depends mainly on the content of a carbonaceous substance in the mould. In the green sand moulds with 5% of seacoal or bonded with furan resin, after the mould filling, a sudden increase in the hydrogen content and the drop of oxygen is observed. This gas evolution results from the oxidation of carbon and reduction of water vapour in the mould material, and also from the reduction of water vapour and alloy reoxidation. In carbon-free sand, the evolution in the gas composition is slower because water vapour is reduced only at the interface. Changes of oxygen and hydrogen content in the controlled zone are determined by the transport phenomena

A R C H I V E S of F O U N D R Y E N G I N E E R I N G Studies of Gas Atmosphere Near the Metal- mould Interface During Casting and Solidification of Ductile Iron

Abstract In sand moulds, at a distance of 3 mm from the metal-mould interface, the sensors of temperature, and of oxygen and hydrogen content were installed. Temperature and the evolution of partial gas pressure have been analysed in moulds bonded with bentonite with or without the addition of seacoal, water glass or furan resin. Moulds were poured with ductile iron. For comparison, also tests with the grey iron have been executed. It was found that the gas atmosphere near the interface depends mainly on the content of a carbonaceous substance in the mould. In the green sand moulds with 5% of seacoal or bonded with furan resin, after the mould filling, a sudden increase in the hydrogen content and the drop of oxygen is observed. This gas evolution results from the oxidation of carbon and reduction of water vapour in the mould material, and also from the reduction of water vapour and alloy reoxidation. In carbon-free sand, the evolution in the gas composition is slower because water vapour is reduced only at the interface. Changes of oxygen and hydrogen content in the controlled zone are determined by the transport phenomena

Adjust an all-terrain vehicle to remote control

W artykule opisano projekt Koła Naukowego "Cyborg++", którego celem było przystosowanie pojazdu typu ATV (All-Terrain Vehicle), nazywanego potocznie quadem, do zdalnego sterowania. Pojazd sterowany jest drogą radiową przez operatora wspomaganego systemem komputerowym. W ramach projektu zautomatyzowano sterowanie quada (obsługę gazu, hamulca, kierownicy oraz innych urządzeń pokładowych), a na jego pokładzie zamontowano mikrokomputer sterujący. W projekcie zastosowano sieć komunikacyjną CAN, zgodnie z obecnymi tendencjami przekazywania i wymiany informacji w pojazdach.This article describes a project conducted by a student scientific circle "Cyborg++". The aim of the project was to adjust an all-terrain vehicle (ATV), informally referred to as a quad, so that it can be remote controlled by a human. The vehicle is controlled by means of radio waves by an operator supported by a computer system. The project also involved automating the quad's control system (the accelerator, the brake, the steering wheel and other onboard devices) as well as installing a controlling microcomputer on the vehicle's board. The CAN bus was used in the project in accordance with the latest trends in mobile vehicles