Influence of feto-pelvic disproportion on milk cows fertility

The aim of the study was to evaluate relations between feto-pelvic proportion and further fertility of milk cows, select proportions and critical values of disproportion best predicting cows with lower fertility as a result of difficult calving as well as to evaluate fertility of cows selected on the basis of chosen criteria. The study was carried out on black-and-white and red-and-white Polish Holstein-Friesian cows in six farms diversified by the number of livestock (from 10 to 20 to over 180 cows) and milk yield (from 6.563 – 9.788 kg/305 days). In total 100 parturitions were analysed (one in each cow) resulting in giving birth to one calf, without complications related to foetus presentation, posture, and position or the need for caesarean section or fetotomy and retained placenta. Analysed parturitions were the result of artificial insemination pregnancies in 53 cases with semen from 33 polish-HF bulls and in 47 cases with semen from 26 the world HF bulls. Considering critical values of chosen feto-pelvic proportions, there is possibility of choosing cows, where more than every third cow had usually delayed over 90 days calving-conception period and more than every fourth became pregnant after at least three inseminations

Contemporary Knowledge on the Assessment of Temperament in Cattle and Its Impact on Production and Reproduction Including Some Immunological, Genetic and Metabolic Parameters

Temperament is associated with the well-being, health, production and reproduction of cattle. In order to increase the population of individuals with the desired temperament, its evaluation should be standardized and be made one of the obligatory elements of breeding and veterinary examination. A number of different tests are used for temperament assessment. In this article, the importance of temperament correlation with some metabolic, genetic, immunological, production and reproductive parameters have been shown, pointing at its influence on the economy and cattle handling. The most common methods for assessing the temperament of cattle are presented, including long-time scales of temperament assessment. At the same time, the relationship of the temperament of cattle with production efficiency, immunity and reproductive indicators has been shown, indicating that its correct assessment is an important aspect of the proper development of the herd and the associated economic growth

The legal aspects of pregnant cows slaughter

Slaughter of pregnant cows is a very controversial issue. In many countries there are no legislative regulations that definitely prohibit slaughter of pregnant females, in others, the regulations limiting their transportation are ineffective. There are also no regulations concerning their fetuses, mainly in the third semester of gestation, that is a very serious issue of animal welfare. Many animal welfare organizations and humane slaughter associations are involved in improving the welfare of livestock during transport and slaughter and they also offer advice to governments and industry to achieve highest worldwide standards in food animal welfare. However, their efforts too often remain unrecognized. The aim of this article was to present the current, available regulations concerning these very important issues

Resynchronisation as an element of improving cattle reproduction efficiency

Oestrus resynchronisation (RES, Resynch) programmes for non-pregnant cows allow shortening the period between an unsuccessful insemination and the next attempt on the same cow. The protocol of oestrus RES may be started after ruling out pregnancy by means of ultrasonography carried out 28 days after insemination or after performing a test for pregnancy-specific glycoproteins (PAG) in blood or milk. The Resynch protocol can be based on a double application of prostaglandins, the OvSynch protocol, or hormonal therapy with exogenous sources of progesterone (CIDR intravaginal devices). The efficiency of the method depends on the functional state of the ovaries, the diameter of the corpus luteum, external factors, and the health and maturity of the cows. The present paper constitutes a comparison of research findings concerning the effectiveness of RES programmes

A Critical Overview on Prostaglandin Inhibitors and Their Influence on Pregnancy Results after Insemination and Embryo Transfer in Cows

Assisted reproductive techniques in cattle, such as artificial insemination (AI) and embryo transfer (ET), are widely used. Despite many years of methodological improvements, the pregnancy rate (PR) in cows has not increased in direct proportion with their development. Among the possibilities to increase the PR is the use of certain steroids and nonsteroidal anti-inflammatory drugs (NSAIDs). The antiluteolytic effect of NSAIDs is achieved by blocking cyclooxygenase, which is involved in the conversion of arachidonic acid to prostaglandins. This article compares the PRs obtained after treatment with the commonly used NSAIDs in cattle, including flunixin meglumine, carprofen, meloxicam, ibuprofen, aspirin, and sildenafil. Studies on the effectiveness of certain steroid drugs on the PR have also been described. The results were not always consistent, and so comparisons between studies were made. In conclusion, flunixin meglumine seems to be an option, and can be recommended for improving ET results, especially in situations of high exposure or susceptibility to stress. Its administration under all circumstances, however, might be pointless and will not lead to the desired effect

Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

A comprehensive description is presented of four novel cases ofamorphus globosus (ag) foetuses originating from multiple pregnancies of Polish Holstein cows

Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

Next-generation sequencing (RNAseq) analysis of gene expression changes during the long-term in vitro culture and osteogenic differentiation of ASCs remains to be important, as the analysis provides important clues toward employing stem cells as a therapeutic intervention. In this study, the cells were isolated from adipose tissue obtained during routine surgical procedures and subjected to 14-day in vitro culture and differentiation. The mRNA transcript levels were evaluated using the Illumina platform, resulting in the detection of 19,856 gene transcripts. The most differentially expressed genes (fold change >|2|, adjusted p value < 0.05), between day 1, day 14 and differentiated cell cultures were extracted and subjected to bioinformatical analysis based on the R programming language. The results of this study provide molecular insight into the processes that occur during long-term in vitro culture and osteogenic differentiation of ASCs, allowing the re-evaluation of the roles of some genes in MSC progression towards a range of lineages. The results improve the knowledge of the molecular mechanisms associated with long-term in vitro culture and differentiation of ASCs, as well as providing a point of reference for potential in vivo and clinical studies regarding these cells’ application in regenerative medicine

In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications

Adipose-derived stem cells (ASCs) exhibiting mesenchymal stem cell (MSC) characteristics, have been extensively studied in recent years. Because they have been shown to differentiate into lineages such as osteogenic, chondrogenic, neurogenic or myogenic, the focus of most of the current research concerns either their potential to replace bone marrow as a readily available and abundant source of MSCs, or to employ them in regenerative and reconstructive medicine. There is close to consensus regarding the methodology used for ASC isolation and culture, whereas a number of molecular analyses implicates them in potential therapies of a number of pathologies. When it comes to clinical application, there is a range of examples of animal trials and clinical studies employing ASCs, further emphasizing the advancement of studies leading to their more widespread use. Nevertheless, in vitro studies will most likely continue to play a significant role in ASC studies, both providing the molecular knowledge of their ex vivo properties and possibly serving as an important step in purification and application of those cells in a clinical setting. Therefore, it is important to consider current methods of ASC isolation, culture, and processing. Furthermore, molecular analyses and cell surface properties of ASCs are essential for animal studies, clinical studies, and therapeutic applications of the MSC properties

Expression of Transforming Growth Factor Beta Isoforms in Canine Endometrium with Cystic Endometrial Hyperplasia–Pyometra Complex

Cystic endometrial hyperplasia (CEH) and pyometra are the most frequently diagnosed uterine diseases affecting bitches of different ages. Transforming growth factor beta (TGF-β) has been classified in females as a potential regulator of many endometrial changes during the estrous cycle or may be involved in pathological disorders. The aim of this study was to determine the expression of TGF-β1, -β2 and -β3 in the endometrium of bitches suffering from CEH or a CEH–pyometra complex compared to clinically healthy females (control group; CG). A significantly increased level of TGF-β1 mRNA expression was observed in the endometrium with CEH–pyometra compared to CEH and CG. Protein production of TGF-β1 was identified only in the endometrium of bitches with CEH–pyometra. An increase in TGF-β3 mRNA expression was observed in all the studied groups compared to CG. The expression of TGF-β2 mRNA was significantly higher in CEH and lower in CEH–pyometra uteri. The results indicate the presence of TGF-β cytokines in canine endometrial tissues affected by proliferative and degenerative changes. However, among all TGF-β isoforms, TGF-β1 could potentially be a key factor involved in the regulation of the endometrium in bitches with CEH–pyometra complex