PHENOTYPIC AND GENOTYPIC ANALYSIS OF A TRUEPERELLA BERNARDIAE STRAIN ISOLATED FROM A DOG

The present study was designed to characterize phenotypically and genotypically the Trueperella bernardiae strain (T. bernardiae P5459/15) isolated from the purulent thelitis of a dog. The species identity could be confirmed by phenotypical investigations, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, and sequencing various molecular targets. Sequencing the 16S rDNA, the β subunit of bacterial RNA polymerase encoding gene rpoBand, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap revealed sequence similarities to the type strain T. bernardiae DSM 9152 of 99.6%, 99.3% and 99.1%, respectively. The present study is the first phenotypic and genotypic characterization of the T. bernardiae strain isolated from a dog

PHENOTYPIC AND GENOTYPIC ANALYSIS OF AN ARCANOBACTERIUM PLURANIMALIUM ISOLATED FROM A MUSKOX (OVIBOS MOSCHATUS)

The present study was designed to characterize an <i>Arcanobacterium pluranimalium</i> strain isolated from a muskox (<i>Ovibos moschatus</i>) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S rRNA gene, the β subunit of bacterial RNA polymerase encoding gene <i>rpoB</i>, the glyceraldehyde 3-phosphate dehydrogenase encoding gene <i>gap</i>, the elongation factor tu encoding gene <i>tuf</i> and the pluranimaliumlysin encoding gene <i>pla<i/> allowed a successful identification of the isolated strain as <i>A. pluranimalium</i>. Gene <i>pla</i> could also be detected by a previously described loop-mediated isothermal amplification (LAMP) assay. This is first report on the isolation and characterization of <i>A. pluranimalium</i> originated from a Muskox