PHENOTYPIC AND GENOTYPIC ANALYSIS OF AN ARCANOBACTERIUM PLURANIMALIUM ISOLATED FROM A MUSKOX (OVIBOS MOSCHATUS)

Abstract

The present study was designed to characterize an <i>Arcanobacterium pluranimalium</i> strain isolated from a muskox (<i>Ovibos moschatus</i>) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S rRNA gene, the β subunit of bacterial RNA polymerase encoding gene <i>rpoB</i>, the glyceraldehyde 3-phosphate dehydrogenase encoding gene <i>gap</i>, the elongation factor tu encoding gene <i>tuf</i> and the pluranimaliumlysin encoding gene <i>pla<i/> allowed a successful identification of the isolated strain as <i>A. pluranimalium</i>. Gene <i>pla</i> could also be detected by a previously described loop-mediated isothermal amplification (LAMP) assay. This is first report on the isolation and characterization of <i>A. pluranimalium</i> originated from a Muskox