Progeny test of germline chimeric roosters by artificial insemination with female Houbara bustards.

<p>Progeny test of germline chimeric roosters by artificial insemination with female Houbara bustards.</p

Primer list for species identification test and molecular sexing.

<p>Primer list for species identification test and molecular sexing.</p

Genotyping analysis of the Houbara bustard family using Houbara bustard microsatellites (STR) markers [26].

<p>*alleles from paternal genome.</p

Parents, offspring and progeny tests.

<p>(<b>a</b>) Germline chimeric rooster; (<b>b</b>) Female Houbara bustard (HB020154); (<b>c</b>) Houbara bustard chick generated from donor cells derived from Houbara bustard sperm produced by chimeric rooster (<b>d</b>) Dead Houbara bustard embryo from chimeric rooster; (<b>e</b>) species identification test by PCR; (<b>f</b>) molecular sexing by PCR of the dead Houbara embryo and the live Houbara chick.</p

PCR gels with species-specific primers showing the detection of Houbara bustard DNA.

<p>(<b>a</b>) Detection of bustard DNA in the gonadal tissue of chimeric chicken embryos. Lanes 3, 7, 10, 13, and 16 show the bustard specific DNA; (<b>b</b>) Species identification sensitivity test: 6×10⁶ chicken sperm mixed with decreasing quantities of bustard sperm; (<b>c</b>) Detection of bustard DNA in the semen of chimeric roosters. Lanes 4, 5, 9, 18, 21, 26 and 28 show the bustard DNA.</p