A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm-0

<p><b>Copyright information:</b></p><p>Taken from "A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm"</p><p>http://www.biomedcentral.com/1472-6750/7/81</p><p>BMC Biotechnology 2007;7():81-81.</p><p>Published online 22 Nov 2007</p><p>PMCID:PMC2241821.</p><p></p>rary (see additional file : Sequence of randomly picked clones)

A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm-1

<p><b>Copyright information:</b></p><p>Taken from "A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm"</p><p>http://www.biomedcentral.com/1472-6750/7/81</p><p>BMC Biotechnology 2007;7():81-81.</p><p>Published online 22 Nov 2007</p><p>PMCID:PMC2241821.</p><p></p>ed in under the control of the T7 promoter. Soluble extracts were prepared, separated by SDS-PAGE, and analyzed by Coomassie staining (a) or Western-blot (b) using 9E10 and an alkaline phosphatase conjugated anti-mouse IgG antibody (substrate BCIP/NBT). Each lane corresponded to 2 × 10cells. The arrow on the left indicates the position of the scFv

A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm-2

<p><b>Copyright information:</b></p><p>Taken from "A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm"</p><p>http://www.biomedcentral.com/1472-6750/7/81</p><p>BMC Biotechnology 2007;7():81-81.</p><p>Published online 22 Nov 2007</p><p>PMCID:PMC2241821.</p><p></p> positive and negative controls, respectively [5]. At 24 h post-transfection, cells were fixed and visualized under a fluorescent microscope with the fluorescein isothiocyanate filter set. The micrographs represent typical fields containing a similar number of cells in each case. Magnification: × 400

A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm-4

<p><b>Copyright information:</b></p><p>Taken from "A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm"</p><p>http://www.biomedcentral.com/1472-6750/7/81</p><p>BMC Biotechnology 2007;7():81-81.</p><p>Published online 22 Nov 2007</p><p>PMCID:PMC2241821.</p><p></p>es represent typical cells transfected with scFv13R4 and three representative anti-histones clones (2, 5 and 10). D, DAPI staining (blue) merged with the GFP signal