Approches biochimiques et bioinformatiques pour l'identification de peptides antimicrobiens d'origine laitière

Une base de données regroupant l'ensemble des peptides antimicrobiens provenant de protéines laitières et décrits dans la littérature a été réalisée, révélant le fait que peu de peptides antimicrobiens ont été identifiés à partir d'un mélange de protéines du lactosérum ou de produits laitiers. D'autre part, un hydrolysat d'isolat de protéines sériques a été fractionné par chromatographie liquide haute performance afin de séparer spécifiquement des fractions contenant des peptides antimicrobiens. Cinq fractions antimicrobiennes ont été obtenues et les peptides responsables de l'activité ont été identifiés. En parallèle, cinq extraits peptidiques hydrosolubles ont été isolés de fromages canadiens. Deux de ces extraits ont révélé une activité antimicrobienne et confirme la présence de peptides antimicrobiens dans les produits laitiers. Les protéines laitières ont ainsi démontré leur capacité à générer des peptides antimicrobiens après hydrolyse

Lead exposure from honey: meta-analysis and risk assessment for the Arab region

The Codex Alimentarius Commission has adopted a maximum level for lead in honey (0.1 mg/kg). Despite representing a population of more than half a billion, exposure and risk assessments for lead specific to the Arab region are lacking. The aim of this work was to collect analytical data for lead in honey available in Arab countries and to assess the risk caused by exposure to lead from these samples for local consumers. A regional mean lead concentration in honey, obtained through a meta-analysis of 57 studies, was used in deterministic risk assessments for adults (nephrotoxicity and cardiovascular effects) and young children (developmental neurotoxicity). A regional pooled mean concentration of 0.12 mg/kg [95%CI: 0.07–0.21] (0.13 mg/kg, if non-detects are considered at their highest possible value) was obtained. Margins of exposure of 363 [71–17182] and 865 [168–40909] were calculated for adults, and of 48 [9–2273] for children, indicating potential concern for the latter population subset. These values were produced using GEMS consumption data, and considering the same intake for both children and adults, potentially overestimating the risk for children. Nevertheless, food competent authorities should consider measures to reduce lead concentration in honey available for sale in the region to prevent trade constraints and to better protect vulnerable populations.</p

Simulated use of thresholds for precautionary allergen labeling: Impact on prevalence and risk

Heterogeneity and overuse of precautionary allergen labelling (PAL) in prepackaged foods have eroded its risk communication efficacy. Experts recommend applying PAL based on allergen concentration thresholds, but adoption remains limited. The aim of this study was to quantitatively assess the potential impact of this approach using Monte Carlo risk simulations. Four allergens and 9 food categories were considered in 2 scenarios: (1) consumption of products currently carrying PAL in Canada where individuals with food allergy (FA) are assumed to consume them, and (2) consumption of products without PAL, in a hypothetical context where PAL is applied based on thresholds that would protect 99 % (ED01) and 95 % (ED05) of individuals with FA, and individuals with FA systematically avoid products with PAL. In scenario (1), although several cases studied would cause 20 reactions/10 000 e.o. Cross-contact milk posed the highest risk (max. 1120 reactions/10 000 e.o.), and peanut, the least (max. 10 reactions/10 000 e.o.). In scenario (2), consumption of products without PAL, when using thresholds for PAL based on ED01, could lead to a maximum of 15 reactions/10 000 e. o. for all studied cases, and based on ED05, to 57 (if excluding dark chocolate with milk PAL). In most cases, the estimated number of reactions per 10 000 e.o. attributed to products with PAL currently on the market would be higher (p < 0.05) than that attributed to products without PAL, if PAL is applied based on the simulated thresholds. Thus, a threshold driven approach to adopt PAL on prepackaged foods, while advising consumers to avoid these products, could be beneficial for individuals with FA in Canada, as products without PAL would result in very few and generally mild adverse reactions

“Vegan” and “plant-based” claims: risk implications for milk- and egg-allergic consumers in Canada

Abstract The market share of foods carrying labels suggesting absence of animal ingredients has significantly increased in recent years. The purpose of this study was to document the purchasing behaviour of egg- or milk-allergic consumers vis-à-vis food marketed as “vegan” and “plant-based”, and to evaluate the associated allergenic risk. A survey was conducted among egg- and milk-allergic consumers and revealed that 86% (margin of error 5.4%; confidence level 95%) would buy “vegan” products, indicating this label may be perceived as a substitute for “free from milk and eggs”. To assess the risk posed by these products, 87 prepackaged foods with “vegan” and/or “plant-based” claims purchased in Quebec were tested for milk proteins, and 64 for egg proteins. Overall, 5.7% and 0% occurrence of milk and egg proteins, respectively, were observed, suggesting that the analysed prepackaged foods carrying “vegan” and/or “plant-based” labels pose little risk to egg- or milk-allergic consumers. However, this is likely due to allergen management practices applied by the Canadian manufacturers of the products tested, and should not be attributed to the use of “vegan” or similar labels. Enhanced regulatory requirements for the use of these labels, and an education campaign on their meaning with respect to allergy-related risks, are necessary to better inform and protect egg- and milk-allergic consumers

Occurrence and risk assessment of sesame as an allergen in selected Middle Eastern foods available in Montreal, Canada

International audienceSesame allergy is a public health problem in many countries around the world. The purpose of this study is to determine the occurrence of sesame allergen in unlabelled or labelled free-sesame Middle Eastern foods with or without Precautionary Allergen Labelling (PAL) 'may contain' and estimate the risk incurred by the Canadian population allergic to sesame with a focus on products purchased in Middle Eastern grocery stores and bakeries in Montreal, Canada. A total of 571 samples were analysed to determine the level of sesame protein. Of the 571 samples analysed, 19% (109/571) contained sesame (results >LOQ) with concentrations of sesame proteins varying between 0.5 and 1,875 mg kg-1 and 35% (199/571) contained traces (a value between LOD and LOQ). Unpackaged products were found to present the highest proportion of sesame containing samples (36%). For packaged products, 16% (27/173) of samples with PAL and 3% (5/173) without PAL were found to contain sesame. A probabilistic approach was used to estimate the risk incurred by the Canadian consumers allergic to sesame. Our evaluation estimated that 33 to 308 allergic reactions may occur out of 10 000 individuals ingesting one type of bakery product contaminated at a level of 0.6-74 mg kg-1 sesame proteins. The incidence and level of sesame cross-contact reported in this study demonstrate that sesame allergic consumers could react if they ignore the precautionary allergen statements on product labels. Attention to sesame as a potential cross-contact agent and as a priority allergen calls for better management, given the growing interest in this ingredient to be included in food formulations. Enhanced risk management efforts must be coupled with targeted risk communication covering both producers and consumers as to the need to adopt and an approach for the application of precautionary allergen labelling based on risk