4 research outputs found
ISSR Analysis of Variability of Cultivated Form and Varieties of Pomegranate (Punica granatum L.) from Azerbaijan
The article presents the results of a study of genetic polymorphism for the first time carried out on
pomegranate varieties and forms of Azerbaijan origin using molecular markers. In total, 102 PCR fragments
were identified, of which 80 were polymorphic. The high level of polymorphism (75.5%) and the rich genetic
diversity were identified among the studied pomegranate collection. As a result of data analysis and on the
basis of the values of the basic parameters (PIC, EMR, MI, RP, MRP) determining informativeness of
markers, all 14 ISSR primers were suitable for genotyping pomegranate accessions. The most effective markers
(UBC808, UBC811, UBC834, and UBC840) were identified among the set of primers tested. A dendrogram
was constructed on the basis of the data obtained, which made it possible to group genotypes into 16
major clusters. The genetic similarity index ranged from 0.032 to 0.94. The study of the genetic relationship
of different pomegranate varieties confirms the effectiveness of the ISSR method, which makes it possible to
determine the level of genetic diversity, as well as to establish the relationship among the studied pomegranate
accessions