13 research outputs found

    Interference Reflection Microscopy

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    Origin of the early-type R stars: a binary-merger solution to a century-old problem?

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    The early-R stars are carbon-rich K-type giants. They are enhanced in C12, C13 and N14, have approximately solar oxygen, magnesium isotopes, s-process and iron abundances, have the luminosity of core-helium burning stars, are not rapid rotators, are members of the Galactic thick disk and, most peculiarly of all, are all single stars. Conventional single-star stellar evolutionary models cannot explain such stars, but mergers in binary systems have been proposed to explain their origin. We have synthesized binary star populations to calculate the number of merged stars with helium cores which could be early-R stars. We find many possible evolutionary channels. The most common of which is the merger of a helium white dwarf with a hydrogen-burning red giant branch star during a common envelope phase followed by a helium flash in a rotating core which mixes carbon to the surface. All the channels together give ten times more early-R stars than we require to match recent Hipparcos observations - we discuss which channels are likely to be the true early-R stars and which are not. For the first time we have constructed a viable model of the early-R stars with which we can test some of our ideas regarding common envelope evolution in giants, stellar mergers, rotation, the helium flash and the origin of the early-R stars

    Transmission electron microscopy study of the cell–sensor interface

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    An emerging number of micro- and nanoelectronics-based biosensors have been developed for non-invasive recordings of physiological cellular activity. The interface between the biological system and the electronic devices strongly influences the signal transfer between these systems. Little is known about the nanoscopic structure of the cell–sensor interface that is essential for a detailed interpretation of the recordings. Therefore, we analysed the interface between the sensor surface and attached cells using transmission electron microscopy (TEM). The maximum possible resolution of our TEM study, however, was restricted by the quality of the interface preparation. Therefore, we complemented our studies with imaging ellipsometry

    Involvement of the Arp2/3 Complex and Scar2 in Golgi Polarity in Scratch Wound Models

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    Cell motility and cell polarity are essential for morphogenesis, immune system function, and tissue repair. Many animal cells move by crawling, and one main driving force for movement is derived from the coordinated assembly and disassembly of actin filaments. As tissue culture cells migrate to close a scratch wound, this directional extension is accompanied by Golgi apparatus reorientation, to face the leading wound edge, giving the motile cell inherent polarity aligned relative to the wound edge and to the direction of cell migration. Cellular proteins essential for actin polymerization downstream of Rho family GTPases include the Arp2/3 complex as an actin nucleator and members of the Wiskott–Aldrich Syndrome protein (WASP) family as activators of the Arp2/3 complex. We therefore analyzed the involvement of the Arp2/3 complex and WASP-family proteins in in vitro wound healing assays using NIH 3T3 fibroblasts and astrocytes. In NIH 3T3 cells, we found that actin and Arp2/3 complex contributed to cell polarity establishment. Moreover, overexpression of N-terminal fragments of Scar2 (but not N-WASP or Scar1 or Scar3) interfere with NIH 3T3 Golgi polarization but not with cell migration. In contrast, actin, Arp2/3, and WASP-family proteins did not appear to be involved in Golgi polarization in astrocytes. Our results thus indicate that the requirement for Golgi polarity establishment is cell-type specific. Furthermore, in NIH 3T3 cells, Scar2 and the Arp2/3 complex appear to be involved in the establishment and maintenance of Golgi polarity during directed migration
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