Studies on substantially increased proteins in follicular fluid of bovine ovarian follicular cysts using 2-D PAGE and MALDI-TOF MS

BACKGROUND: The objective of this study was to identify substantially increased proteins in bovine cystic follicular fluid (FF) in order to clarify the pathology and etiology of bovine ovarian follicular cysts (BOFC). METHODS: Proteins in normal and cystic FF samples were subjected to two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and were compared using silver stained gel images with PDQuest image analysis software. Peptides from these increased spots were analyzed by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), and were identified based on the NCBI database by a peptide mass fingerprinting method. RESULTS: Comparative proteomic analysis showed 8 increased protein spots present in cystic FF. MS analysis and database searching revealed that the increased proteins in cystic FF were bovine mitochondrial f1-atpase (BMFA), erythroid associated factor (EAF), methionine synthase (MeS), VEGF-receptor, glyceraldehydes 3-phosphate dehydrogenase (GAPDH), heat shock protein 70 (HSP70), β-lactoglobulin (BLG) and succinate dehydrogenase Ip subunit (SD). CONCLUSION: Our results suggest that these proteins are overexpressed in BOFC, and that they may play important roles in the pathogenesis of BOFC. Furthermore, these proteins in the FF could be useful biomarkers for BOFC

Actiniaria Hertwig 1882

Order Actiniaria Hertwig, 1882 Superfamily Actinioidea Rafinesque, 1815 [Japanese name: umeboshi-isoginchaku-jouka] Family Haloclavidae Verrill, 1899 [Japanese name: kombo-isoginchaku-ka] Haloclavidae Verrill, 1899: 41; Carlgren 1949: 29. Type genus. Haloclava Verrill, 1899.Published as part of Izumi, Takato, 2021, The Largest Cnidae Among the Sea Anemones; Description of a New Haloclavid Species from Japan, Haloclava hercules (Cnidaria: Actiniaria: Enthemonae: Haloclavidae), pp. 241-247 in Species Diversity 26 on page 242, DOI: 10.12782/specdiv.26.241, http://zenodo.org/record/573902

Description of three new species of Scolanthus (Cnidaria, Anthozoa, Actiniaria, Edwardsiidae): first records of the genus from Japan

Scolanthus is one genus of Edwardsiidae, a speciose family of burrowing worm-like sea anemones characterized by lacking a physa-like aboral end and by possessing nemathybomes on the whole body except at the distal end. This genus has been recorded worldwide, but there have been no specimens collected from Japan. In this study, we discovered four Scolanthus species in Japan for the first time: Scolanthus armatus (Carlgren, 1931) and Scolanthus kopepe sp. n. from the Ogasawara Islands, Scolanthus ena sp. n. from Ena Bay, Kanagawa, and Scolanthus isei sp. n. from Sugashima Island, Mie

Two species of Edwardsia having gigantic nematocysts, E. aff. tuberculata and E. alternobomen sp. nov. (Cnidaria; Anthozoa; Actiniaria; Edwardsiidae) from Japan

Izumi, Takato, Fujita, Toshihiko (2019): Two species of Edwardsia having gigantic nematocysts, E. aff. tuberculata and E. alternobomen sp. nov. (Cnidaria; Anthozoa; Actiniaria; Edwardsiidae) from Japan. Zootaxa 4661 (3): 533-544, DOI: https://doi.org/10.11646/zootaxa.4661.3.

Gems of the southern Japanese seas – four new species of Edwardsianthus (Anthozoa, Actiniaria, Edwardsiidae) with redescriptions of two species

Edwardsianthus England, 1987 is a genus of Edwardsiidae, a family of burrowing and worm-like sea anemones characterized by lacking four mesenteries in the first cycle and containing only one type of nematocysts in nemathybomes. Until now, this genus has accommodated only two species since its establishment and has been recorded only from Indo-West Pacific regions. In this study, six species are reported from Japan: two are previously known species, E. pudicus (Klunzinger, 1877) and E. gilbertensis (Carlgren, 1931); four are new species, E. carbunculus sp. nov., E. sapphirus sp. nov., E. smaragdus sp. nov., and E. amethystus sp. nov. Based on these results, the diagnostic features of the genus are revised

Fig. 3 in Antennapeachia jambio (Cnidaria: Actiniaria: Haloclavidae), the Second Species of Genus Antennapeachia, with Revision of the Diagnosis of the Genus

Fig. 3. Schematic representation of the arrangements of the tentacles (A) and mesenteries (B) in Antennapeachia jambio sp. nov. There are two antenna tentacles and fourteen marginal tentacles in the cycles, and one tentacle communicates with each endo- and exocoel (A and M means the position of antenna and marginal tentacle, respectively). The antenna tentacles are between the ventrolateral mesenteries. Two microcnemes (broken line) disappear near the mouth. (Position: the left or right of the page, nearby the Result part.)Published as part of <i>Izumi, Takato, Fujita, Toshihiko & Yanagi, Kensuke, 2017, Antennapeachia jambio (Cnidaria: Actiniaria: Haloclavidae), the Second Species of Genus Antennapeachia, with Revision of the Diagnosis of the Genus, pp. 109-115 in Species Diversity 22</i> on page 112, DOI: 10.12782/sd.22_109, <a href="http://zenodo.org/record/10093015">http://zenodo.org/record/10093015</a&gt

Antennapeachia jambio (Cnidaria: Actiniaria: Haloclavidae), the Second Species of Genus Antennapeachia, with Revision of the Diagnosis of the Genus

Izumi, Takato, Fujita, Toshihiko, Yanagi, Kensuke (2017): Antennapeachia jambio (Cnidaria: Actiniaria: Haloclavidae), the Second Species of Genus Antennapeachia, with Revision of the Diagnosis of the Genus. Species Diversity 22: 109-115, DOI: 10.12782/sd.22_10

Fig. 2 in Antennapeachia jambio (Cnidaria: Actiniaria: Haloclavidae), the Second Species of Genus Antennapeachia, with Revision of the Diagnosis of the Genus

Fig. 2. Antennapeachia jambio sp. nov., holotype, CMNH-ZG 06546, external morphology in living state (A, B) and internal morphology of the preserved specimen (C–I). (A) Oral view; the conchula is located on the ventral side of the mouth. (B) Lateral view of the whole body. (C) Cross section of the column. (D) Longitudinal section of a tentacle. (E) Cross section of a tentacle showing the ectodermal longitudinal muscles (indicated by arrowheads). (F) Cross section of the column, enlargement of a part of (C). (G) Longitudinal section of the oral end of the column. (H) Cross section of the mesentery with filament. (I) Longitudinal section of the aboral end. Abbreviations: a, actinopharynx; at, antenna tentacle; c, conchula; f, filament; me, mesoglea; mi, microcneme; mt, marginal tentacle; pm, parietal muscle; rm, retractor muscle; s, siphonoglyph; tcm, tentacular circular muscle; tlm, tentacular longitudinal muscle. Scale bars indicate 5 mm in A and B, 1 mm in C, and 100 µm in D–I. (Position: the center of upper or lower of the page, nearby the Result part.)Published as part of <i>Izumi, Takato, Fujita, Toshihiko & Yanagi, Kensuke, 2017, Antennapeachia jambio (Cnidaria: Actiniaria: Haloclavidae), the Second Species of Genus Antennapeachia, with Revision of the Diagnosis of the Genus, pp. 109-115 in Species Diversity 22</i> on page 111, DOI: 10.12782/sd.22_109, <a href="http://zenodo.org/record/10093015">http://zenodo.org/record/10093015</a&gt