Expression of Estrogen Receptor Coactivator Proline-, Glutamic Acid- and Leucine-Rich Protein 1 within Paraspinal Muscles in Adolescents with Idiopathic Scoliosis - Fig 5

<p><b>Western blotting PELP1 protein expression in paravertebral muscles (A)</b>. Ovarian cancer was used as an external control. Western blotting GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) protein expression was used as internal control (B). Designations: 1 –Page Ruler^TM protein ladder, 2 –deep paravertebral muscles concave side, 2 –deep paravertebral muscles convex side, 3 –control (ovarian cancer), 4 –superficial muscles.</p

Characteristics of the patients.

<p>Characteristics of the patients.</p

Immunohistochemical localization of PELP1 protein expression in paravertebral muscles.

<p>(A, B) Light microscopic images with regular hematoxylin and eosin staining were applied to assess the tissue morphology. The transverse section (C) and the longitudinal section (D) of the deep paravertebral muscle with positive reaction to PELP1 within the nucleus. Control reaction: (E) positive control–ovarian cancer, (F) negative control–paravertebral muscles incubation without primary antibodies.</p

Relative <i>PELP1</i> expression level in deep paravertebral muscles and superficial muscles.

<p>Different superscript letters above each bar are significantly different, P<0.05 (Kruskal-Wallis test with Dunn’s post hoc test).</p