Collagen matricryptin promotes cardiac function by mediating scar formation

This is an open access article under the CC BY-NC-ND license.Aims A peptide mimetic of a collagen-derived matricryptin (p1159) was shown to reduce left ventricular (LV) dilation and fibrosis after 7 days delivery in a mouse model of myocardial infarction (MI). This suggested p1159 long-term treatment post-MI could have beneficial effects and reduce/prevent adverse LV remodeling. This study aimed to test the potential of p1159 to reduce adverse cardiac remodeling in a chronic MI model and to elucidate p1159 mode-of-action. Materials and methods Using a permanent occlusion MI rodent model, animals received p1159 or vehicle solution up to 28 days. We assessed peptide treatment effects on scar composition and structure and on systolic function. To assess peptide effects on scar vascularization, a cohort of mice were injected with Griffonia simplicifolia isolectin-B4. To investigate p1159 mode-of-action, LV fibroblasts from naïve animals were treated with increasing doses of p1159. Key findings Matricryptin p1159 significantly improved systolic function post-MI (2-fold greater EF compared to controls) by reducing left ventricular dilation and inducing the formation of a compliant and organized infarct scar, which promoted LV contractility and preserved the structural integrity of the heart. Specifically, infarcted scars from p1159-treated animals displayed collagen fibers aligned parallel to the epicardium, to resist circumferential stretching, with reduced levels of cross-linking, and improved tissue perfusion. In addition, we found that p1159 increases cardiac fibroblast migration by activating RhoA pathways via the membrane receptor integrin α4. Significance Our data indicate p1159 treatment reduced adverse LV remodeling post-MI by modulating the deposition, arrangement, and perfusion of the fibrotic scar.ECU Open Access Publishing Support Fun

Dose-dependent Inhibition of SNAT4 Transport Activity by DTT and TCEP.

<p>Xenopus oocytes expressing wild type SNAT4 were preincubated with DTT (0–10 mM) (A) or TCEP (0–10 mM) (B) for 30 min. [³H] L-alanine uptake assay was then performed in the presence of DTT or TCEP. Water injected oocytes were used as a negative control. L-alanine uptake was significantly decreased in a dose-dependent manner compared to untreated control. Data is presented as mean ± SEM, n = 3 (∼ 10 oocytes/sample). DTT or TCEP at 1 and 10 mM versus untreated control of SNAT4, ***, P<0.001.</p

Disruption of the disulfide bridge partially loses L-arginine transport function of SNAT4.

<p>cRNA of the wild type, C18A, C232A, C345A or C321A mutant was injected into <i>Xenopus</i> oocytes and subjected to [³H] L-arginine uptake assays. Water injected oocytes were used as a negative control. Data is presented as mean ± SEM, n = 3 (∼10 oocytes/sample). Mutants versus WT, *, P<0.05.</p

Residues Cys-249 and Cys-321 are functionally involved in transport function of SNAT4.

<p>(A) DNA constructs containing single cysteine site mutants, C18A, C232A, C249A, C321A and C345A were generated by PCR using WT SNAT4 as a template. cRNAs were injected in Xenopus oocytes. The transport activity was determined and the data was normalized with the protein expression. The transport activity of both C249A and C321A mutants was completely abolished. Data is presented as mean ± SEM, n = 3 (∼ 10 oocytes/sample). All mutants versus WT, ***, P<0.001. (B) DNA construct containing 3 cysteine to alanine mutations with retained Cys-249 and Cys-321 residues (C18A, C232A, C345A) was generated by site directed mutagenesis and the transporter activity was determined by uptake assay. After normalization with total protein expression of SNAT4 variants in oocyte (left panel), the mutant showed partial recovery in L-alanine transport as compared to the water injected control. Data is presented as mean ± SEM, n = 3 (∼10 oocytes/sample). The mutant versus WT, ***, P<0.001.</p

Cys-null mutant of SNAT4 completely loses transport function, but is capable of expressing on the cell surface.

<p>(A) The locations of the 5 cysteine residues are indicated (arrows) in the determined topological structure of SNAT4 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056792#pone.0056792-Shi1" target="_blank">[12]</a>. (B) cRNA of the wild type and Cys-null mutant was injected into the oocytes and subjected to [³H] L-alanine uptake assays. Water injected oocytes were used as a negative control. The transporter activity obtained was normalized with the protein expression data. Data is presented as mean ± SEM, n = 3 (∼10 oocytes/sample). Cys-null versus WT, ***, P<0.001. (C) Xenopus oocytes injected with wild type and Cys-null mutant cRNA were surface biotinylated with NHS-SS-Biotin. Biotinylated proteins and the pre-loaded cell lysates (pre-loading) were also immunoblotted with anti-SNAT4 antibody or anti-pan-actin antibody. The ratio of biotinylated versus total SNAT4 was quantified and the data is presented as mean ± SEM, n = 20. The levels of SNAT4 expressed on the cell surface and corresponding pre-loaded SNAT4 were quantified by Scion Image software and the percentage of biotinylated versus total SNAT4 was calculated. Data is presented as mean ± SEM, n = 3 (∼ 20 oocytes/sample). All mutants versus WT, ***, P<0.001.</p