MOESM3 of An automated, cost-effective and scalable, flood-and-drain based root phenotyping system for cereals

Additional file 3: Figure S3. Illustration of the outcome of the image segmentation process of a barley seedling grown in the system. Shoot (A) and root (B) photographs were taken with a digital camera against a dark homogeneous background

MOESM4 of An automated, cost-effective and scalable, flood-and-drain based root phenotyping system for cereals

Additional file 4: Table S1. Amount of compounds used for the preparation of Hoagland’s medium No. 2 Basal Salt Mixture. The pH of the medium was adjusted to ~ 5,9-6,1° using 1 N NaOH

The effect of the Al-treatment on root system parameters evaluated after 1- day and 7 days of the exposition.

<p>Tested Al³⁺ doses consisted of: 10; 20; 30 μM AlCl₃, in full Hoagland’s medium, pH = 4 (treatments), pH = 6 (control). The measured parameters include: total root length (A), total root area (B), total root volume (C), average root diameter (D). Different letters above the bars indicate statistically significant differences (ANOVA; p < 0.05) between combinations. The percentages above the bars indicate the reduction/ increase of the value in comparison to the control.</p

The frequency of root meristematic cells of barley cv. ‘Sebastian’ at S-phase in control and Al-treated roots.

<p>The error bars represent the standard deviations of the mean. The significant differences (P < 0.05) between the treated combinations and the controls (pH 4 and pH 6) within the same treatment day are indicated by a capital letter A (1-day treatment) or B (7-day treatment). The frequency of S-phase cells is not significantly different (P < 0.05) within the same treatment day for different pH.</p

The total root length of barley seedlings cv. ‘Sebastian’ treated with different concentrations of Al for 7 days.

<p>Tested Al³⁺ doses included 5; 10; 20; 40; 60 μM AlCl₃, in full Hoagland’s medium, pH = 4 (treatments), pH = 6 (control). The measurements were carried out using WinRHIZO system.</p

Examples of the detection of S-phase cells using EdU incorporation in roots of barley cv. ‘Sebastian’ seedlings treated with different concentrations of Al for 1 and 7 days.

<p>(A-C) DAPI staining (A`-C`) Alexa Fluor 647 fluorescence (red) in S-phase cells detected after EdU incorporation. Bars represent 20 μm.</p

The frequency of root cells of barley cv. ‘Sebastian’ with micronuclei and damaged nuclei in control and after Al treatment.

<p>The error bars represent the standard deviations of the mean. The significant differences (P < 0.05) between the treated combinations and the control within the same treatment day are indicated by capital letters: A, B, D, E.</p

AlCl₃ concentrations selected for the treatment of barley seedlings grown in DWC hydroponics.

<p>The calculations of available Al³⁺ concentrations in the full Hoagland’s nutrient solution, pH = 4 were calculated using GEOCHEM-EZ software.</p

The mitotic activity of root cells of barley seedlings cv. ‘Sebastian’—(control and Al treated).

<p>The error bars represent the standard deviations of the mean. The significant differences (P < 0.05) between the treated combinations and the control within the same treatment day, are indicated by a capital letter A (1-day treatment) or B (7-day treatment).</p

The frequency of labelled nuclei in root cells of barley cv. ‘Sebastian’ in TUNEL test in control and after Al treatment.

<p>The error bars represent the standard deviations of the mean. The significant differences (P < 0.05) between the treated combinations and the control within the same treatment day are indicated by a capital letter A (1-day treatment) or B (7-day treatment).</p