Influence of Stitch Density and Stitch Thread Thickness on Compression After Impact Strength of Stitched Composites

This study aims to investigate the influence of stitch density and stitch thread thickness on compression after impact (CAI) strength of stitched composites. Unstitched laminated composites and specimens stitched with varying stitch density and stitch thread thickness are subjected to impact damage and then compressive loading. It is shown that stitched composites have higher CAI strength than unstitched counterpart due to smaller impact-induced delamination area, where local buckling occurs during compressive failure. However, it is revealed that the effectiveness of stitching in suppressing delamination growth and inhibiting sublaminate buckling under compressive loading is intimately related to stitch density. It is also found out that stitch thread thickness has little influence on CAI strength at low impact energy level, but has considerable effect at high impact energy level

Validation of Delamination Reduction Trend for Stitched Composites using Quasi-Static Indentation Test

A novel empirical-based Delamination Reduction Trend (DRT) for stitched composites has been recently proposed. The DRT is capable of predicting the effective reduction in impact induced delamination area due to the influence of stitching. DRT simply relates two parameters: normalized delamination area and stitch fibre volume fraction, to characterize the effectiveness of stitching in impact damage suppression. This paper seeks to validate the DRT by using quasi-static indentation (QSI) test, which is considered analogous to low velocity impact test, due to similar structural response. Results from QSI test show good agreement with DRT. Furthermore, limitations in DRT have been established

Progressive Damage in Stitched Composites under Impact Loading

Damage in carbon fibre reinforced plastics (CFRP) due to impact loading is an extremely complex phenomenon that comprises of multiple failure mechanisms like intra-laminar matrix cracks, interlaminar delamination, fibre pull-out and fibre fracture. In stitched composites, impact damage behavior is further complicated by the presence of through-thickness stitching [1, 2], which not only favorably increases mode I/II interlaminar strength [3, 4], but also inevitably creates geometrical defects like weak resin-rich pockets around stitch threads and misalignment of in-plane fibres. Computational modeling has been used to simulate progressive damage effectively [5]. However, the complexity of impact damage progression in stitched composites would need to be first understood and appreciated by physical experimental observations. In this study, quasi-static indentation (QSI) test is performed for the first time on stitched composites. QSI offers a good validation and comparison with low-velocity impact (LVI) test [6], and provides good understanding on damage progression in composite structures under impact loading. Damage initiation, propagation and ultimate failure are investigated due to the effect of stitching, particularly the influence of stitch density. Nondestructive evaluation (NDE) techniques namely ultrasonic c-scan analysis, x-ray radiography and xray micro computed tomography are employed to elucidate various damage mechanisms in stitched composites

Material Selection of Z-fibre in Stitched Composites - Experimental and Analytical Comparison Approach

Strain energy release rates are measured and compared for laminated composites stitched with different fibre materials – Carbon, Kevlar and Vectran. DCB test and FE simulation are performed to evaluate the interlaminar toughness. It is proven that Vectran provides the toughest interlaminar reinforcement and is most suitable for Zfibre application

Development of a simultaneous analytical method for five conjugated cholesterol metabolites in urine and investigation of their performance as diagnostic markers for Niemann-Pick disease type C

Niemann-Pick disease type C (NPC) is an autosomal recessive disorder characterized by progressive nervous degeneration. Because of the diversity of clinical symptoms and onset age, the diagnosis of this disease is difficult. Therefore, biomarker tests have attracted significant attention for earlier diagnostics. In this study, we developed a simultaneous analysis method for five urinary conjugated cholesterol metabolites, which are potential diagnostic biomarkers for a rapid, convenient, and noninvasive chemical diagnosis, using liquid chromatography/tandem mass spectrometry (LC/MS/MS). By the method, their urinary concentrations were quantified and the NPC diagnostic performances were evaluated. The developed LC/MS/MS method showed high accuracy and and satisfied all analytical method validation criteria. Analyzing the urine of healthy controls and patients with NPC, three of five urinary conjugated cholesterol metabolites concentrations corrected by urinary creatinine were significantly higher in the patients with NPC. As a result of receiver operating characteristics analysis, the urinary metabolites might have excellent diagnostic marker performance. 3β-sulfooxy-7β-hydroxy-5-cholenoic acid showed particularly excellent diagnostic performance with both 100% clinical sensitivity and specificity, suggesting that it is a useful NPC diagnostic marker. The urinary conjugated cholesterol metabolites exhibited high NPC diagnostic marker performance and could be used for NPC diagnosis

Structural Determination of Lysosphingomyelin-509 and Discovery of Novel Class Lipids from Patients with Niemann–Pick Disease Type C

Niemann–Pick disease type C (NPC) is an autosomal recessive disorder caused by the mutation of cholesterol-transporting proteins. In addition, early treatment is important for good prognosis of this disease because of the progressive neurodegeneration. However, the diagnosis of this disease is difficult due to a variety of clinical spectrum. Lysosphingomyelin-509, which is one of the most useful biomarkers for NPC, was applied for the rapid and easy detection of NPC. The fact that its chemical structure was unknown until recently implicates the unrevealed pathophysiology and molecular mechanisms of NPC. In this study, we aimed to elucidate the structure of lysosphingomyelin-509 by various mass spectrometric techniques. As our identification strategy, we adopted analytical and organic chemistry approaches to the serum of patients with NPC. Chemical derivatization and hydrogen abstraction dissociation–tandem mass spectrometry were used for the determination of function groups and partial structure, respectively. As a result, we revealed the exact structure of lysosphingomyelin-509 as N-acylated and O-phosphocholine adducted serine. Additionally, we found that a group of metabolites with N-acyl groups were increased considerably in the serum/plasma of patients with NPC as compared to that of other groups using targeted lipidomics analysis. Our techniques were useful for the identification of lysosphingomyelin-509

The classification of irreducible admissible mod p representations of a p-adic GL_n

Let F be a finite extension of Q_p. Using the mod p Satake transform, we define what it means for an irreducible admissible smooth representation of an F-split p-adic reductive group over \bar F_p to be supersingular. We then give the classification of irreducible admissible smooth GL_n(F)-representations over \bar F_p in terms of supersingular representations. As a consequence we deduce that supersingular is the same as supercuspidal. These results generalise the work of Barthel-Livne for n = 2. For general split reductive groups we obtain similar results under stronger hypotheses.Comment: 55 pages, to appear in Inventiones Mathematica

Epstein-Barr Virus BGLF4 Kinase Retards Cellular S-Phase Progression and Induces Chromosomal Abnormality

Epstein-Barr virus (EBV) induces an uncoordinated S-phase-like cellular environment coupled with multiple prophase-like events in cells replicating the virus. The EBV encoded Ser/Thr kinase BGLF4 has been shown to induce premature chromosome condensation through activation of condensin and topoisomerase II and reorganization of the nuclear lamina to facilitate the nuclear egress of nucleocapsids in a pathway mimicking Cdk1. However, the observation that RB is hyperphosphorylated in the presence of BGLF4 raised the possibility that BGLF4 may have a Cdk2-like activity to promote S-phase progression. Here, we investigated the regulatory effects of BGLF4 on cell cycle progression and found that S-phase progression and DNA synthesis were interrupted by BGLF4 in mammalian cells. Expression of BGLF4 did not compensate Cdk1 defects for DNA replication in S. cerevisiae. Using time-lapse microscopy, we found the fate of individual HeLa cells was determined by the expression level of BGLF4. In addition to slight cell growth retardation, BGLF4 elicits abnormal chromosomal structure and micronucleus formation in 293 and NCP-TW01 cells. In Saos-2 cells, BGLF4 induced the hyperphosphorylation of co-transfected RB, while E2F1 was not released from RB-E2F1 complexes. The E2F1 regulated activities of the cyclin D1 and ZBRK1 promoters were suppressed by BGLF4 in a dose dependent manner. Detection with phosphoamino acid specific antibodies revealed that, in addition to Ser780, phosphorylation of the DNA damage-responsive Ser612 on RB was enhanced by BGLF4. Taken together, our study indicates that BGLF4 may directly or indirectly induce a DNA damage signal that eventually interferes with host DNA synthesis and delays S-phase progression