Living on the Edge: Border Countries Should Have Strict Veterinary and Health Policy on Leishmaniasis

First human and canine cases as well as presence of competent Phlebotomine fly vectors are reported for the first time in Slovenia. Number of infected dogs in Slovenia has been increasing in the last few years. Having increased number of infected dogs and a presence of proven Phlebotomine fly vectors at the same time in a climatically suitable region may lead to endemic spread of the disease. And that is the kind of situation calling for governmental regulation. Basic preparedness and rapid response mechanisms should be in place. Leishmaniasis cases should be detected early and reaction should be quick. In epidemic-prone areas and before the anticipated outbreak season, the responsibilities of the outbreak task force members should be defined; the necessary needs for response, surveillance, and control should be assessed; the surveillance system should be reinforced; criteria for epidemic alert should be set up; and all health facilities should be provided with minimum stocks of basic diagnostic and treatment supplies. Successful preventive measures should include regular veterinary checks of all imported dogs and dogs traveling outside the country, vector control, use of effective repellents, sleeping indoors with nets on the windows, and antileishmanial vaccination of dogs

Illegal waste sites as a potential micro foci of mediterranean leishmaniasis

Apart from being against the law, illegal waste dumping also poses a threat to human health and to the environment. Solid and decomposing waste is an ideal breeding ground for a number of rodents, insects, and other vermin that pose a health risk through the spread of infectious diseases. The main objective of this study was to survey disease vectors and rodents for the presence of Leishmania sp. from waste sites along the Istrian Peninsula in Slovenia and Croatia

Upotreba lasera u konzervaciji papira

The authors of this artical describe and discuss about analytical laser THUNDER ART technique applied to the diagnostics of archival art work on paper in the case study of Architectural Blueprint done by Nikola Krasnov (1864-1939) that belongs to the collection of The Archives of Yugoslavia. Authors particularly wanted to characterize laser cleaning as comfortable and effective technique for the process of impurities removal in the conservation of works on paper. Obtained results had shown that green wavelength had removed only repainted parts of colors, mostly red and black.U ovom radu razmatra se upotreba lasera u konzervaciji papira na primjeru arhitektonskog crteža na ozalidu arhitekta Nikole Krasnova (1864.-1939.) koji se čuva u Arhivu Jugoslavije u Beogradu. Crtež je čišćen laserom THUNDER ART sa sve tri valne dužine, a najbolje se pokazala zelena koja je uklonila upravo preslikane dijelove boja, ponajviše crvenu i crnu, zbog kojih je i provedeno ovo čišćenje. Autori zaključuju da je lasersko čišćenje udobna i učinkovita tehnika uklanjanja nečistoća u postupku konzervacije djela na papiru

Upotreba lasera u konzervaciji papira

The authors of this artical describe and discuss about analytical laser THUNDER ART technique applied to the diagnostics of archival art work on paper in the case study of Architectural Blueprint done by Nikola Krasnov (1864-1939) that belongs to the collection of The Archives of Yugoslavia. Authors particularly wanted to characterize laser cleaning as comfortable and effective technique for the process of impurities removal in the conservation of works on paper. Obtained results had shown that green wavelength had removed only repainted parts of colors, mostly red and black.U ovom radu razmatra se upotreba lasera u konzervaciji papira na primjeru arhitektonskog crteža na ozalidu arhitekta Nikole Krasnova (1864.-1939.) koji se čuva u Arhivu Jugoslavije u Beogradu. Crtež je čišćen laserom THUNDER ART sa sve tri valne dužine, a najbolje se pokazala zelena koja je uklonila upravo preslikane dijelove boja, ponajviše crvenu i crnu, zbog kojih je i provedeno ovo čišćenje. Autori zaključuju da je lasersko čišćenje udobna i učinkovita tehnika uklanjanja nečistoća u postupku konzervacije djela na papiru

Evidence of Aujeszky s disease in wild boar in Serbia

Background: Aujeszkys disease is a viral disease of suids caused by Suid Herpesvirus 1. The disease has worldwide distribution with significant economic impact. In Serbia, there is neither an Aujeszkys disease eradication nor national vaccination programme of domestic pigs. Since clinical symptoms of Aujeszkys disease are not specific, it is important to establish a link between clinical signs and presence of ADV active infection in wild boars. The aim of this study was to investigate the possibility of active infection within wild boar showing signs of ADV and also to examine relationship between isolates from domestic pigs and wild boar. Having in mind that virus has not been previously isolated from wild boars in Serbia, we report the first isolation of Suid Herpesvirus 1 from this species in Serbia. Results: Tissue and serum samples from 40 wild boars from eastern Serbia were examined for evidence of Aujeszkys disease (AD). Suid Herpesvirus 1 (SHV1), the cause of AD was isolated on PK15 cell line from three tissue samples, inducing cytopathic effect (CPE) with syncytia forming, and viral genome was detected by polymerase chain reaction (PCR) in eight samples. Genetic analysis of us4, us9 and ul49.5 partial sequences showed high homology between ADV isolates from wild boars and between isolates from wild boars and domestic animals. Neutralizing antibodies were not detected by virus neutralisation test (VNT) in sera from four out of eight PCR positive wild boars suggesting recent infection in those animals. Conclusions: This is the first demonstration of Aujeszkys disease virus (ADV) in the wild boar population in Serbia although seroconversion has been detected previously