Relatedness network for pairs of isolates inferred IBD over <i>Pfcrt</i>.

<p>Each node identifies a unique isolate and an edge is drawn between two isolates if they were inferred either partially or completely IBD over <i>Pfcrt</i>. Isolates with MOI = 1 are represented by circles while isolates with MOI > 1 are represented by squares. There are 178 clusters in this network comprising of 1,563 isolates in total, with the largest cluster containing 1,134 isolates. Isolates that are not IBD over <i>Pfcrt</i> are omitted from the network. (A) Isolates are coloured according to country. (B) Isolates are coloured if they carry the K76T mutation associated with chloroquine resistance.</p

The proportion of pairs within each country who are IBD at each SNP.

<p>Chromosome boundaries are indicated by grey dashed vertical lines and positive control genes are identified by tick marks on the top x-axis. Countries that are part of the African continent are shades of red and orange while countries in Southeast Asia are shades of blue and Papua New Guinea is pink.</p

Relatedness networks for pairs of isolates inferred IBD over the interval chr6: 1,001,000–1,300,000.

<p>Each node identifies a unique isolate and an edge is drawn between two isolates if they were inferred IBD anywhere over this interval. Isolates with MOI = 1 are represented by circles while isolates with MOI > 1 are represented by squares. There are 93 clusters in this network comprising of 1,862 isolates in total, with the largest cluster containing 1,643 isolates. Isolates that are not IBD over this interval omitted from the network.</p

Relatedness network for pairs of isolates inferred IBD over <i>Pfk13</i>.

<p>Each node identifies a unique isolate and an edge is drawn between two isolates if they were inferred either partially or completely IBD over <i>Pfk13</i>. Isolates with MOI = 1 are represented by circles while isolates with MOI > 1 are represented by squares. There are 242 clusters in this network comprising of 1,148 isolates in total, with the largest cluster containing 335 isolates. Isolates that are not IBD over <i>Pfk13</i>are omitted from the network. (A) Isolates are coloured according to country. (B) Isolates are coloured if they carry the C580Y mutation associated with artemisinin resistance.</p

Selection signals in Ghana stratified by pairs who are IBD or non-IBD over <i>Pfmdr1</i>.

<p>Pairs that are IBD over <i>Pfmdr1</i> represent the red signal while pairs that are not IBD over <i>Pfmdr1</i> represent the black signal. The dashed horizontal line represent a 5% singificance threshold and the dashed vertical lines idenitfies the chromosome boundaries.</p

Relatedness network for pairs of isolates identified as having high proportions of IBD sharing.

<p>Each node identifies a unique isolate and an edge is drawn between two isolates if they share more than 90% of their genome IBD. Isolates with MOI = 1 are represented by circles while isolates with MOI > 1 are represented by squares. There are 264 clusters in this network comprising 805 isolates (out of 2,377 isolates) in total. Isolates that do not share more than 90% of their genome IBD with any other isolate are omitted from the network.</p

Selection signals from isoRelate on Pf3k dataset.

<p>–log₁₀(p-values) of X_iR calculated by transforming and normalizing the IBD proportions within each country. Dashed horizontal lines represent a 5% singnificance threshold. Grey dashed vertical lines indicate chromosome boundaries. Positive control genes are identified by gene symbol and tick marks on the upper x-axis.</p

Power and accuracy of isoRelate to detect IBD in simulated sequencing data for <i>P</i>. <i>falciparum</i>.

<p>The performance results are segregated by the clonal-fraction of the related clone in the isolate. Clones that make up the highest proportion of an isolate are referred to as the major clone, while those that make up the smallest proportion are the minor clone. For MOI = 3 isolates, the clone that is neither the major nor the minor clone is referred to as the middle clone.</p

Additional file 1: of The treatment of non-malarial febrile illness in Papua New Guinea: findings from cross sectional and longitudinal studies of health worker practice

Full list of recorded diagnoses for the outpatient surveillance sample. (DOCX 21 kb

Power and accuracy results of isoRelate, iHS and HaploPS in detecting complex sweeps.

<p>The performance results are segregated by sweep type, where the results for selection on standing variation are shown for a selection coefficient of 0.1. Power is defined as the proportion of sweeps (calculated over 10 reps) with at least one 20 kb bin within 50 kb either side of the selected SNP that either contains three or more significant SNPs (isoRelate and iHS, alpha = 5%), or is in the top 1% of bins with respect to the average number of haplotype counts per bin (haploPS), as a function of the number of generations since the sweep was introduced. Accuracy is calculated as either the proportion of 20 kb bins with at least three significant SNPs (isoRelate and iHS) that are within 50kb of the selected SNP or the proportion of 20 kb bins within the top 1% of bins with respect to of haplotype counts (haploPS), that are within 50kb of the selected SNP, as a function of the number of generations since the sweep was introduced. If there are no bins with at least three significant SNPs for any of the 10 reps then the accuracy is set to NA.</p