Comparative screening of the anti-oxidant and antimicrobial activities of Sempervivum marmoreum L. extracts obtained by various extraction techniques

This paper presents a comparative study of the anti-oxidant and anti-microbial activities, total phenolic compounds and total flavonoids in extracts obtained from houseleek (Sempervivum marmoreum L.) leaves by the classical (maceration), ultrasonic and Soxhlet extraction (CE, UE and SE, respectively). The extract obtained by the CE contained higher amounts of phenolic and flavonoid compounds and showed a better antioxidant activity than those obtained using other two techniques. All the extracts, independent of the extraction technique applied, showed antimicrobial activities against Aspergillus niger and Candida albicans only but not against the tested bacteria

The Ocular Conjunctiva as a Mucosal Immunization Route: A Profile of the Immune Response to the Model Antigen Tetanus Toxoid

Background: In a quest for a needle-free vaccine administration strategy, we evaluated the ocular conjunctiva as an alternative mucosal immunization route by profiling and comparing the local and systemic immune responses to the subcutaneous or conjunctival administration of tetanus toxoid (TTd), a model antigen. Materials and methods: BALB/c and C57BL/6 mice were immunized either subcutaneously with TTd alone or via the conjunctiva with TTd alone, TTd mixed with 2% glycerol or TTd with merthiolate-inactivated whole-cell B. pertussis (wBP) as adjuvants. Mice were immunized on days 0, 7 and 14 via both routes, and an evaluation of the local and systemic immune responses was performed two weeks after the last immunization. Four weeks after the last immunization, the mice were challenged with a lethal dose (2 x LD50) of tetanus toxin. Results: The conjunctival application of TTd in BALB/c mice induced TTd-specific secretory IgA production and skewed the TTd-specific immune response toward a Th1/Th17 profile, as determined by the stimulation of IFN gamma and IL-17A secretion and/or the concurrent pronounced reduction of IL-4 secretion, irrespective of the adjuvant. In conjunctivaly immunized C57BL/6 mice, only TTd administered with wBP promoted the establishment of a mixed Th1/Th17 TTd-specific immune response, whereas TTd alone or TTd in conjunction with glycerol initiated a dominant Th1 response against TTd. Immunization via the conjunctiva with TTd plus wBP adjuvant resulted in a 33% survival rate of challenged mice compared to a 0% survival rate in non-immunized animals (p lt 0.05). Conclusion: Conjunctival immunization with TTd alone or with various adjuvants induced TTd-specific local and systemic immune responses, predominantly of the Th1 type. The strongest immune responses developed in mice that received TTd together with wBP, which implies that this alternative route might tailor the immune response to fight intracellular bacteria or viruses more effectively

PRODUCT AESTHETICS FOR MECHANICAL ENGINEERS

This paper gives a scope of course called Product aesthetics at Mechanical faculty, University in Belgrade, Serbia. During this course students are learning about standards and laws in process of product design. With this knowledge students are able to create more profound ability of aesthetic evaluation and form aesthetic judgement of in creating certain mechanical product such as car, motorcycle etc. Practical part of this course includes creating a product by using a 3D CAD software which has both aesthetic value and practical usage. Students are presenting that product to other students and they give their evaluation grade

STEREOGRAPHIC PROJECTION OF THE HEAVENS OVER BELGRADE USED FOR THE CONSTRUCTION OF ASTRONOMICAL CITY CLOCK

There are many different ways of rendering 3D objects into two dimensions. Different kinds of projections are able to represent things like size, areas, distances or perspective, realistically. One particular kind of projection used for representing spheres and circles on spheres in two dimensions (i.e. some maps of the earth or celestial sphere) is stereographic projection. Stereographic projection has two important characteristics that differentiate it from other kinds of projections: it preserves circles and angles. This paper discloses the construction of the stereographic projection of the celestial sphere over Belgrade as the clock face of the astronomical clock. This astronomical clock will be capable to present the motion, as seen from the Earth, of the Sun, Moon, Ecliptic and the phases of the Moon during the year. Moreover, the hours of the sunrise, noon and sunset will be clearly observed on the stereographic dial of the astronomical clock

The proliferation indices (PI) of TTd-stimulated SMLN cells from BALB/c and C57BL/6 mice immunized via the conjunctiva according to the assigned immunization protocol.

<p>The numbers of viable SMLN cells were assessed by MTT assay following a 48 h cultivation in 10% FCS/50 µM β-mercaptoethanol/RPMI 1640 medium supplemented or not with TTd (5 µg/ml). PIs were calculated for each mouse. The results are presented as the mean PI ± SE for each experimental group (n = 10). The statistical significance of the differences in PIs between groups treated according to the assigned protocols was determined by <i>t</i>-test (<i>P<</i>0.05*, <i>P<</i>0.005**). The reference group is indicated by a dotted line, and the comparison group is indicated by an arrow.</p

Levels of TTd-specific SIgA in tear washes from BALB/c and C57BL/6 mice that were immunized according to the assigned protocols.

<p>Samples were collected two weeks after the completion of the immunizations and were assayed by ELISA (dilution 1∶2). The results are presented as the mean A_492/620± SE (n = 10). The significance of the observed differences was calculated by <i>t</i>-test (<i>P<0.05*</i>, <i>P<0.005**</i>). The reference group is indicated by a dotted line, and the comparison group is indicated by an arrow.</p

Levels of IFNγ (A), IL-4 (B), IL-17A (C) and IL-10 (D) in the supernatants from <i>in vitro</i> TTd-stimulated SMLN cells obtained from age-matched control mice (n.c.) and mice immunized via the conjunctiva according to the assigned protocol (bars).

<p>SMLN cells were cultivated at 37°C in 5% CO₂ for 48 h in 10% FCS/RPMI 1640/50 µM β-mercaptoethanol supplemented with 5 µg/ml TTd. The levels of cytokines in the supernatants of corresponding SMLN cells incubated in 10% FCS/RPMI 1640/50 µM β-mercaptoethanol under similar conditions (non-stimulated cells) are indicated by solid lines. The results are presented as the mean concentration ± SE (n = 10). Concentrations of cytokines in supernatants of TTd-stimulated cultures were compared by <i>t-</i>tests. The reference group is indicated by a dotted line, and the comparison group is indicated by an arrow. A <i>t</i>-test was also used for the comparison of cytokine concentration in supernatants of corresponding non-stimulated and TTd-stimulated cultures, and the statistical significance of the differences is marked next to the solid bar, indicating the level of the cytokine within the non-stimulated culture. The levels of statistical significance are assigned as follows: <i>P<0.05*</i>, <i>P<0.005**</i>, <i>P<0.0005***</i>_.</p

IgG1/IgG2a (A) and IgG1/IgG2c (B) ratios calculated for TTd-specific antibodies in the sera of TTd-immunized BALB/c and C57BL/6 mice, respectively.

<p>Serum samples were collected two weeks after the completion of the immunizations and were assayed by ELISA (dilution 1∶100). Ratios were determined using the A₄₀₅ values obtained upon the measurement of TTd-specific IgG1 and IgG2a or IgG2c in sera. The results are presented as the mean A₄₀₅(IgG1)/A₄₀₅(IgG2a,c) ± SE (n = 10). The significance of the differences between syngeneic mice immunized subcutaneously with TTd (reference group) and those immunized via the conjunctiva was calculated by <i>t</i>-test (<i>P<</i>0.05*, <i>P<</i>0.005**, <i>P<</i>0.0005***).</p

The relative abundance (RA) of TTd-specific mIgG⁺ B cells within the total population of mIgG⁺ B cells in SMLN upon completion of the assigned immunization protocols.

<p>The RA of the TTd-specific mIgG⁺ B cell population was calculated for each mouse. The results are presented as the mean RA ± SE for each experimental group of mice (n = 5). The statistical significance of the observed differences in TTd-specific mIgG⁺ B cell pool abundances was determined by <i>t</i>-test (<i>P<0.05*</i>, <i>P<0.005**</i>, <i>P<0.0005***</i>). The reference group is indicated by a dotted line, and an arrow indicates the comparison group.</p