Expression of cell surface markers characterizing MSCs.

<p>(A) Expression of MSCs markers on the surface of mesenchymal stem cells of group of responders versus non responders (** - statistic significance is ≤ 0.01; * - statistic significance is ≤ 0.02). (B) Expression of MSCs markers on the surface of mesenchymal stem cells of group of diabetic patients (n=13) versus non diabetic (n=9).</p

Secretion of factors from MSCs and gene expression.

<p>(<b>A</b>) Secretome comparison of several factors of responders (n=27) and non-responders (n=14) CLI patients; For detection of cytokines, chemokines and growth factors Bio-Plex Pro Human Cytokine 27-plex Assay from Bio-Rad was used. The concentration of each factor was calculated per mg of total proteins in 24 hour conditioned medium. The values of growth factors detected in control medium were deducted from values found in conditioned medium. (B) Relative expression of 15 genes typical for MSCs on the level of proteins. Cell extracts of MSCs in early passage of seven non-responders and eight responders were examined by Proteome Profiler Human Pluripotent Stem Cell Array.</p

Doubling time of continually cultivated MSCs from CLI patients in comparison to healthy young donor.

<p>(<b>A</b>) Ability of MSCs of CLI patient to differentiate to osteogenic, adipogenic and chondrogenic lineages; (<b>B</b>) Doubling time of MSCs of CLI patients in first passage. The value is an average of two independent estimations; (<b>C</b>) Doubling time of continually cultivated MSCs from four CLI patients in comparison to healthy young donor. Lines represent the trend.</p

Additional file 1: of Characteristics of responders to autologous bone marrow cell therapy for no-option critical limb ischemia

Presents the dataset supporting the conclusions of this article. (XLSX 26 kb