1 research outputs found
Growth-Factor-Releasing Polyelectrolyte Multilayer Films to Control the Cell Culture Environment
Polyelectrolyte multilayers
(PEMs) are of great interest as cell
culture surfaces because of their ability to modify topography and
surface energy and release biologically relevant molecules such as
growth factors. In this work, fibroblast growth factor 2 (FGF2) was
adsorbed directly onto polystyrene, plasma-treated polystyrene, and
glass surfaces with a polyÂ(methacrylic acid) and poly-l-histidine
PEM assembled above it. Up to 14 ng/cm<sup>2</sup> of FGF2 could be
released from plasma-treated polystyrene surfaces over the course
of 7 days with an FGF2 solution concentration of 100 μg/mL applied
during the adsorption process. This release rate could be modulated
by adjusting the adsorption concentration, decreasing to as low as
2 ng/cm<sup>2</sup> total release over 7 days using a 12.5 μg/mL
FGF2 solution. The surface energy and roughness could also be regulated
using the adsorbed PEM. These properties were found to be substrate-
and first-layer-dependent, supporting current theories of PEM assembly.
When released, FGF2 from the PEMs was found to significantly enhance
fibroblast proliferation as compared to culture conditions without
FGF2. The results showed that growth factor release profiles and surface
properties are easily controllable through modification of the PEM
assembly steps and that these strategies can be effectively applied
to common cell culture surfaces to control the cell fate