Investigation of spectroscopic properties of mono and Di-styryl indole-containing BODIPYs

In the current study, two novel mono- and distyryl BODIPYs have been successfully synthesized employing the Knoevenagel type condensation reaction between 1,3,5,7?tetramethyl BODIPY and 2,3-diphenyl-4,6-dimethoxyindole-7-carbaldehyde. This C[sbnd]C bond extension approach leads to change in hybridization of methyl carbon from sp3 to sp2 which play quite important role in the photophysical properties of BODIPYs by allowing extended conjugation. The absorption and fluorescence spectroscopy were used to determine the photophysical properties include molar extinction coefficient, fluorescence lifetime and fluorescence quantum yield. Moreover, the effect of solvent polarity on targeted BODIPY units were analysed with experimental investigations. The Density Functional Theory (DFT)-optimized structures were consisted with the experimental data obtained from the single-crystal X-ray structures. © 2022 Elsevier B.V.1895206, 2094181; Gebze Teknik Üniversitesi: 2018-A105-35This work has been supported by Research Found of the Gebze Technical University (Project Number: 2018-A105-35). The numerical calculations reported in this paper were partially performed at TUBITAK ULAKBIM, High Performance and Grid Computing Center (TRUBA resources). IE acknowledges Omer Tayfuroglu and Cem Gol, for their fruitful comments/discussions on the quantum chemical calculations. The authors wish to thank Dr. A. Ingendoh and Dr. A. Koch for MALDI-TOF-MS analyses. The CCDC number 1895206 for 4 and 2094181 for 5 contain the supplementary crystallographic data (CIF) for this article. These data can be obtained free of charge from the Director, CCDC, 12 Union Road, Cambridge CB2 1EZ, UK (Fax: +44-1223-336-033; e-mail: [email protected]. ac.uk or http://www.ccdc.cam.ac.uk).This work has been supported by Research Found of the Gebze Technical University (Project Number: 2018-A105-35). The numerical calculations reported in this paper were partially performed at TUBITAK ULAKBIM, High Performance and Grid Computing Center (TRUBA resources). IE acknowledges Omer Tayfuroglu and Cem Gol, for their fruitful comments/discussions on the quantum chemical calculations. The authors wish to thank Dr. A. Ingendoh and Dr. A. Koch for MALDI-TOF-MS analyses. The CCDC number 1895206 for 4 and 2094181 for 5 contain the supplementary crystallographic data (CIF) for this article. These data can be obtained free of charge from the Director, CCDC, 12 Union Road, Cambridge CB2 1EZ, UK (Fax: +44-1223-336-033; e-mail: [email protected]. ac.uk or http://www.ccdc.cam.ac.uk)

Gastroprotective activity of Polygonum chinense aqueous leaf extract on ethanol-induced hemorrhagic mucosal lesions in rats

Polygonum chinense is aMalaysian ethnic plant with various healing effects. This study was to determine preventive effect of aqueous leaf extract of P. chinense against ethanol-induced gastric mucosal injury in rats. Sprague Dawley rats were divided into seven groups. The normal and ulcer control groups were orally administered with distilled water. The reference group was orally administered with 20mg/kg omeprazole. The experimental groups received the extracts 62.5, 125, 250, and 500 mg/kg, accordingly. After sixty minutes, distilled water and absolute ethanol were given (5 mL/kg) to the normal control and the others,respectively. In addition to histology, immunohistochemical and periodic acid schiff (PAS) stains, levels of lipid peroxidation,malondialdehyde (MDA), antioxidant enzymes, and superoxide dismutase (SOD) were measured. The ulcer group exhibited severe mucosal damages. The experimental groups significantly reduced gastric lesions and MDA levels and increased SOD level. Immunohistochemistry of the experimental groups showed upregulation and downregulation of Hsp70 and Bax proteins, respectively. PAS staining in these groups exhibited intense staining as compared to the ulcer group. Acute toxicity study revealed the nontoxic nature of the extract. Our data provide first evidence that P. chinense extract could significantly prevent gastric ulcer

Application of neurite orientation dispersion and density imaging (noddi) to a tau pathology model of alzheimer's disease

Increased hyperphosphorylated tau and the formation of intracellular neurofibrillary tangles are associated with the loss of neurons and cognitive decline in Alzheimer's disease, and related neurodegenerative conditions. We applied two diffusion models, diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI), to in vivo diffusion magnetic resonance images (dMRI) of a mouse model of human tauopathy (rTg4510) at 8.5months of age. In grey matter regions with the highest degree of tau burden, microstructural indices provided by both NODDI and DTI discriminated the rTg4510 (TG) animals from wild type (WT) controls; however only the neurite density index (NDI) (the volume fraction that comprises axons or dendrites) from the NODDI model correlated with the histological measurements of the levels of hyperphosphorylated tau protein. Reductions in diffusion directionality were observed when implementing both models in the white matter region of the corpus callosum, with lower fractional anisotropy (DTI) and higher orientation dispersion (NODDI) observed in the TG animals. In comparison to DTI, histological measures of tau pathology were more closely correlated with NODDI parameters in this region. This in vivo dMRI study demonstrates that NODDI identifies potential tissue sources contributing to DTI indices and NODDI may provide greater specificity to pathology in Alzheimer's disease