4-(1,2,4-Triazol-1-yl)aniline

In the title compound, C8H8N4, the dihedral angle between the triazole ring [maximum deviation = 0.003 (1) Å] and the benzene ring is 34.57 (7)°. In the crystal, mol­ecules are linked into sheets lying parallel to the ac plane via inter­molecular N—H⋯N and C—H⋯N hydrogen bonds. Aromatic π–π [centroid–centroid distance = 3.6750 (8) Å] stacking and N—H⋯π inter­actions are also observed

Seroprevalence Study of Human Brucellosis by Conventional Tests and Indigenous Indirect Enzyme-Linked Immunosorbent Assay

Brucellosis is one of the most important reemerging zoonoses in many countries. Brucellosis is caused by Gram-negative coccobacillus belonging to genus Brucella. Human brucellosis often makes the diagnosis difficult. The symptoms and clinical signs most commonly reported are fever, fatigue, malaise, chills, sweats headaches, myalgia, arthralgia, and weight loss. Some cases have been presented with only joint pain, lower backache, and involuntary limb movement, burning feet, or ischemic heart attacks. The focus of this work was to develop a highly sensitive and specific indirect ELISA by using smooth lipopolysaccharide antigen of Brucella abortus 99 to detect anti-Brucella antibodies at Project Directorate on Animal Disease Monitoring and Surveillance. Serum samples collected from 652 individuals in whom fever was not the major symptom but the complaint was of joint pain, headache, lower backache, and so forth, were screened by Rose Bengal plate agglutination test (RBPT) and standard tube agglutination test (STAT). Subsequent testing of sera by indigenous indirect ELISA detected 20 samples positive (3.6% seroprevalence), and indirect ELISA was found to be more sensitive than RBPT and STAT. The seroprevalence in South Karnataka was 2.14%, and in North Karnataka it was 0.92%

Ethyl 1-cyclo­hexyl-5-(4-meth­oxy­phen­yl)-1H-pyrazole-4-carboxyl­ate

In the title compound, C19H24N2O3, the benzene ring forms a dihedral angle of 65.34 (7)° with the pyrazole ring. The cyclo­hexane ring adopts a chair conformation. In the crystal, mol­ecules are linked into a inversion dimers by pairs of C—H⋯O hydrogen bonds, generating R 2 2(22) ring motifs

(2E)-3-(1,3-Diphenyl-1H-pyrazol-4-yl)-1-phenyl­prop-2-en-1-one

In the title compound, C24H18N2O, the pyrazole ring is essentially planar [maximum deviation = 0.004 (1) Å] and makes dihedral angles of 18.07 (4), 48.60 (4) and 9.13 (5)° with the phenyl rings. In the crystal, adjacent mol­ecules are connected via inter­molecular C—H⋯O hydrogen bonds, forming dimers. Furthermore, the crystal structure is stabilized by weak C—H⋯π and π–π inter­actions, with centroid–centroid distances of 3.6808 (5) Å

5-[(4-Meth­oxy­benz­yl)sulfan­yl]-2-methyl-1,3,4-thia­diazole

The title mol­ecule, C11H12N2OS2, is twisted with a dihedral angle of 83.63 (12)° between the 1,3,4-thia­diazole and benzene rings. The meth­oxy group deviates slightly from the attached benzene ring, with a C—C—O—C torsion angle of 4.2 (4)°. In the crystal, mol­ecules are linked by weak C—H⋯N inter­actions and stacked along the c axis

4-(3-Chloro­phen­yl)-3-[(2,6-difluoro­benz­yl)sulfan­yl]-5-(3,4,5-trimeth­oxy­phen­yl)-4H-1,2,4-triazole

In the title compound, C24H20ClF2N3O3S, the essentially planar triazole ring (r.m.s. deviation = 0.001 Å) forms dihedral angles of 22.35 (10), 68.17 (10) and 42.01 (10)° with the mean planes of the trimeth­oxy­phenyl, chloro­phenyl and difluoro­phenyl rings, respectively. A weak intra­molecular C—H⋯π inter­action occurs. In the crystal, mol­ecules are linked into sheets lying parallel to the bc plane by C—H⋯O and C—H⋯N hydrogen bonds. The crystal packing also features weak C—H⋯π inter­actions

4-{[(E)-(3-Phenyl-1H-pyrazol-4-yl)methyl­idene]amino}-1H-1,2,4-triazole-5(4H)-thione

In the title compound, C12H10N6S, a weak intra­molecular C—H⋯S hydrogen bond stabilizes the mol­ecular conformation. The pyrazole and triazole rings form a dihedral angle of 17.82 (8)°. The mol­ecule adopts an E configuration with respect to the central C=N double bond. In the crystal, inter­molecular N—H⋯N and N—H⋯S hydrogen bonds link mol­ecules into chains propagating in [20]

(E)-1-(2,4-Dichloro­phen­yl)-3-(1,3-diphenyl-1H-pyrazol-4-yl)prop-2-en-1-one

In the title mol­ecule, C24H16Cl2N2O, the dihedral angles between the pyrazole ring and its N- and C-bonded phenyl rings are 7.06 (10) and 53.15 (10)°, respectively. The dihedral angle between the two pendant rings is 52.32 (10)°. The mol­ecule exists in a trans conformation with respect to the acyclic C=C bond. In the crystal, inversion dimers occur in which each mol­ecule is linked to the other by two C—H⋯O hydrogen bonds to the same acceptor O atom. There are also short Cl⋯Cl contacts [3.3492 (9) Å] and C—H⋯π inter­actions

Ethyl 1,5-diphenyl-1H-pyrazole-4-carboxyl­ate

The asymmetric unit of the title compound, C18H16N2O2, contains two independent mol­ecules (A and B). In mol­ecule A, the pyrazole ring is inclined at angles of 48.86 (6) and 60.80 (6)° with respect to the two phenyl rings; the corresponding angles for mol­ecule B are 46.86 (6) and 58.63 (6)°. In the crystal, mol­ecules of type A are linked into sheets parallel to (001) via weak C—H⋯O hydrogen bonds, whereas the mol­ecules of type B are linked into chains along [010] via weak C—H⋯O hydrogen bonds

3-(2,4-Dichloro­phen­yl)-5-phenyl-1,2,4-oxadiazole

In the title compound, C14H8Cl2N2O, the dihedral angles between the plane of the oxadiazole ring and those of the benzene rings are 2.3 (1) and 9.5 (1)°. In the crystal, mol­ecules are linked into chains along the c axis by C—H⋯Cl inter­actions. These chains are stacked along the a axis