5 research outputs found
Gene expression of selected apoptotic markers in HSC-3 treated with myrmecodia pendans plant extract
Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract
Introduction: Myrmecodia pendans (M. pendans), also known as Sarang Semut is an epiphyte with
potential as a new therapeutic agent. Recently, we reported that M. pendans induced apoptotic
activity in human oral squamous carcinoma (HSC-3) cell line. This study aimed to study how M.
pendans extract affected apoptotic markers BAX/BCL-2 expression.
Materials and methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was
allowed to dry thoroughly, then extracted aqueously. The extract was kept at -80 ยฐC before freeze
drying. The crude extract was used in the experiments. HSC-3 cells were cultured in Dulbeccoโs
Modified Essential Medium, then exposed to M. pendans at 2.5, 5.0 and 7.5 mg/ml for 24 and 48 h.
Doxorubicin
(0.005 mg/ml) was used as positive control. BAX and BCL-2 expression was analysed by Quantitative
Real Time Polymerase Chain Reaction.
Results: BAX and BCL-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, highest
levels of BAX and BCL-2 were observed with M. pendans at 2.5 mg/ml, while downregulation was
observed at 5 mg/ml. However, after 48 h, BAX and BCL-2 were downregulated at all concentrations.
The ratio of BAX/ BCL-2 in HSC-3 cells at 24 h showed significant upregulation at all treatment
concentrations, but was downregulated after 48 h.
Conclusion: M. pendans extract induced apoptotic activity in HSC-3 cells. This study suggests that
induction of apoptosis in HSC-3 cell line is regulated via the pro-apoptotic (BAX) and anti-
apoptotic (BCL
-2) pathways
Gene Expression of Selected Apoptotic Markers in Human Oral Squamous Carcinoma HSC-3 Cell Line Treated with Myrmecodia Pendans Plant
This poster was submitted to participate at the 11th Annual ISCC Conference and Congress organised by the Indonesian Society of Cancer and Chemopreventive, held on 9-21 November 2020
Biology 1 (bio0415) workbook
Biology I (Bio415) Workbook for BIO0415 course. This book is specially written for foundation students taking Biology at Centre for Foundation Studies, IIUM. The purpose of this workbook is to provide students with exercises to help them understand topics in Biology
This Biology I Workbook consists of multiple-choice questions and structure questions for 14 chapters. Answer schemes and feedbacks are provided at the end of each chapter as guidance for the students to understand the concepts. The questions in each chapter were tested on students and moderated to confirm the validity
Biology 1 workbook
This Biology I Workbook for Biology I course is specially written for foundation students taking Biology I at Centre for Foundation Studies, IIUM. The purpose of this workbook is to provide students with exercises to help them understand topics in Biology I. This Biology I Workbook consists of multiple-choice questions and structure questions for 13 chapters. Answer schemes and feedbacks are provided at the end of each chapter as guidance for the students to understand the concepts. The questions in each chapter were tested on students and moderated to confirm the validity