37 research outputs found
Retrospective real-life efficacy assessment of teneligliptin in Indian T2DM patients
Background: Teneligliptin is been introduced recently in Indian market and data available are limited on Indian patients. Hence, the hospital based real life retrospective evaluation was planned out to evaluate, the efficacy of teneligliptin in type 2 diabetes mellitus in Indian population. Hence study was designed, a retrospective evaluation, of efficacy of teneligliptin in type 2 diabetes mellitus in Indian population.Methods: Data of 775 patients, who were prescribed teneligliptin was collected from hospital records. Teneligliptin 20mg was prescribed to all patients who were uncontrolled on other OHAs and for a mean duration of 8 weeks. Parameter evaluated in this study were change in FBG, PPBG and HbA1c from the baseline at 8week. With profile of outcome i.e. response and failure rates were also assessed with respect to age, gender, BMI and duration of diabetes.Results: Of 775 patients were enrolled, 427 were males and 348 females. The average age was 53.04 years among the study population. The mean duration of diabetes was 23 months. There was significant change in HbA1c, fasting and postprandial blood glucose levels at 8 week of teneligliptin therapy. Changes in HbA1c, FPG and PPG from baseline to end of study were-1.22±1.12% (p=0.001), -35.8±25.5mg/dl (p=0.001) and -60.7±28.6mg/dl (p=0.001) respectively. Out of 775 patients, 106 (13,7%) were non-responders where it was further sub analysed with different parameter such as age, gender, BMI and duration of diabetes in order to observe response of teneligliptin in diabetic patients.Conclusions: This real life retrospective evaluation showed efficacy of teneligliptin in real world scenario. It can be an effective alternative to conventional gliptins available for prescription in India
Invasive papillary carcinoma of the male breast: Report of a rare case and review of the literature
Breast masses occur in men far less commonly than women. Papillary
lesions of the male breast are rare and comprise a spectrum of lesions
ranging from benign intraductal papilloma to intraductal papillary
carcinoma and invasive papillary carcinoma. In this case report, a
78-year-old man presented with a subareolar painless mass. Fine needle
aspiration cytology (FNAC) was performed. Cytologic examination
revealed a cellular aspirate. A diagnosis of papillary lesion favoring
papillary carcinoma was rendered. The patient underwent modified
radical mastectomy, which showed invasive papillary carcinoma. As far
as we know, only a few cases of invasive papillary carcinoma of the
male breast have been published in the literature. To the best of our
knowledge, this is the first case report of invasive papillary
carcinoma of male breast in Malaysia. In this purview, we discuss
papillary carcinoma of male breast with review of the relevant
literature
Splenic infarct as a diagnostic pitfall in radiology
Follow-up of colorectal carcinoma after therapy is based on symptoms,
tumor markers, and imaging studies. Clinicians sometimes face
diagnostic dilemmas because of unusual presentations on the imaging
modalities coupled with rising serum markers. We report a case of
colorectal carcinoma that presented with gastrointestinal symptoms 14
months after completion of treatment. Investigations showed rise in
carcinoembryonic antigen (CEA). Suspecting disease recurrence, complete
radioimaging workup was performed; the only abnormality detected was a
smooth, hypodense area in the posterior third of the spleen on
contrast-enhanced computed tomography abdomen. In view of the previous
diagnosis of carcinoma colon, the symptoms reported by the patient, the
elevated CEA, and the atypical CECT appearance, a diagnosis of splenic
metastasis was made. The patient was subjected to splenectomy as a
curative treatment. However, the histopathological report revealed it
to be a splenic infarct. The present case reemphasizes the limitations
of radiological studies in the follow-up of carcinoma colon
The use of two-dimensional spectroscopy to interpret the effect of temperature on the near infrared spectra of whisky
The variations in temperature during the analysis of alcoholic beverages are of importance to develop protocols based on near infrared spectroscopy. The objective of this study was to evaluate the effect of increasing temperature on the near infrared spectra of whisky samples using two-dimensional correlation spectroscopy. Whisky samples from different commercial labels were analyzed at four different temperatures (25 degrees C, 35 degrees C, 45 degrees C, and 55 degrees C) using a UV-VIS-NIR instrument. Asynchronous and synchronous two-dimensional correlation spectroscopy was used to reveal the effect of temperature on the near infrared spectra of the samples. The results of this study indicated that temperatures between 40 degrees C and 55 degrees C alter absorption at specific wavelengths in the near infrared region of the whisky samples analyzed. The combination of near infrared spectroscopy with two-dimensional correlation spectroscopy has the potential to dramatically improve the ef?ciency of analytical laboratories, considering the range of data that can be collected
Splenic infarct as a diagnostic pitfall in radiology
Follow-up of colorectal carcinoma after therapy is based on symptoms,
tumor markers, and imaging studies. Clinicians sometimes face
diagnostic dilemmas because of unusual presentations on the imaging
modalities coupled with rising serum markers. We report a case of
colorectal carcinoma that presented with gastrointestinal symptoms 14
months after completion of treatment. Investigations showed rise in
carcinoembryonic antigen (CEA). Suspecting disease recurrence, complete
radioimaging workup was performed; the only abnormality detected was a
smooth, hypodense area in the posterior third of the spleen on
contrast-enhanced computed tomography abdomen. In view of the previous
diagnosis of carcinoma colon, the symptoms reported by the patient, the
elevated CEA, and the atypical CECT appearance, a diagnosis of splenic
metastasis was made. The patient was subjected to splenectomy as a
curative treatment. However, the histopathological report revealed it
to be a splenic infarct. The present case reemphasizes the limitations
of radiological studies in the follow-up of carcinoma colon
Genome-wide mapping of DNA double-strand breaks from eukaryotic cell cultures using Break-seq
Summary: We describe a genome-wide DNA double-strand break (DSB) mapping technique, Break-seq. In this protocol, we provide step-by-step instructions for cell embedment in agarose, in-gel DSB labeling and subsequent capture, followed by standard Illumina library construction and sequencing. We also provide the framework for sequence data processing and DSB peak identification. Finally, we present a custom-designed 3D-printed device for processing agarose-embedded DNA samples. The protocol is applicable to Saccharomyces cerevisiae, as well as mammalian suspension, adherent, and 3D organoid cell cultures.For complete details on the use and execution of this protocol, please refer to Hoffman et al. (2015) and Chakraborty et al. (2020)
Influence of the scanning temperature on the classification of whisky samples analysed by UV-VIS spectroscopy
The definition of the optimal temperature and its effects (either increasing or variations) during analysis of alcoholic beverages are of importance to develop protocols based in spectroscopy. Although several reports have been published on the use of spectroscopy combined with chemometrics to classify and authenticate alcoholic beverages (e.g., wine, tequila, whisky), few reports deal with issues related with the spectra collection (e.g., temperature, path length) and its effect on the classification performances. The objective of this study was to evaluate the effect of increasing temperature on both the UV-VIS spectra of whisky and on the classification results of the samples according to country of origin. Whisky samples from different commercial labels were analysed at different temperatures (25, 35, 45, 55 â—¦C) using a UV-VIS instrument (Agilent, Cary 3500). Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models based in cross validation were used to classify whisky samples according to scanning temperature and origin. The results of this study indicated that temperature did not affect the classification of whisky samples according to country of origin. Overall, well defined protocols need to be defined for routine use of these methods in research and by the industr