Medaka inbred strains: past, present and future

メダカ生物遺伝資源の標準化に関する国際シンポジウ

Morphogenesis of the medaka cerebellum, with special reference to the mesencephalic sheet, a structure homologous to the rostrolateral part of mammalian anterior medullary velum.

We have examined cerebellar morphogenesis after neural tube stage in medaka (Oryzias latipes), a ray-finned fish, by conventional histology and immunohistochemistry using anti-proliferating cell nuclear antigen (PCNA) and anti-acetylated tubulin antibodies. Our results indicate that the medaka cerebellum is formed in the four successive stages: 1) formation and enlargement of the cerebellar primordia, 2) rostral midline fusion of the left/right halves of the cerebellar primordia, 3) formation of the cerebellar matrix zones in the midline and caudalmost regions of the primitive cerebellum, and 4) growth and differentiation of the cerebellum. Our results also show that cerebellar morphogenesis is different from that in mammals in three important points: the developmental origins of the primordia, directions along which cerebellar fusion proceeds, and number, locations and duration of the cerebellar matrix zones. During the course of this study, an alar-derived membranous structure between the cerebellum and the midbrain in the adult medaka brain was identified as the structure homologous to the rostrolateral part of the mammalian anterior medullary velum. We have named this structure in the adult teleostean brains as the mesencephalic sheet. The present study indicates that there exist both conserved and divergent patterns in cerebellar morphogenesis in vertebrates

Definitions of and nomenclature for medaka strains

メダカ生物遺伝資源の標準化に関する国際シンポジウ

Development of a genetic monitoring system for medaka inbred strains

メダカ生物遺伝資源の標準化に関する国際シンポジウ

Medaka Biology, Management, and Experimental Protocols Volume 2

The second volume of Medaka: Biology, Management, and Experimental Protocols, together with the first volume, helps to familiarize scientists with the advantages of using medaka in experimental designs, to facilitate research using medaka, and to stimulate progress by adopting medaka as a model animal. The second edition expands on the first by providing additional information and current protocols that have been recently developed, or modified, to successfully raise medaka fish under stable culture conditions in the laboratory.This volume explores new technologies developed after 2009, using the fish as a molecular tool in the fields of life science, evolution, ecology, and toxicology. The authors―noted experts in the field―provide the latest information that spans the varied research disciplines and addresses the value to science of medaka\u27s adoption as a model animal. This important book:Explores the advantages of using medaka in experimental designs, to facilitate researchDetails the most recent protocols to successfully raise medaka fish under stable conditions in the laboratoryExplores the most recent developments in the fieldProvides step-by step specifics for each protocol, allowing researchers to adapt them for use in their own workWritten for students and researchers in fish biology and aquaculture, Medaka: Biology, Management, and Experimental Protocols, Volume 2 introduces the cutting edge research in basic and applied biology using medaka as a model animal as well as descriptions of experimental methods and protocols