Higher School Teacher’s Competences and “Electronic” Pedagogical Culture in the Post-Pandemic World

During the period of the forced remote learning caused by the threat of the coronavirus infection spread, Russian university teachers, as well as their foreign colleagues, had to acquire quickly new skills in the field of information technologies and organization of classes exclusively in electronic mode. However, the changes in the structure of the teachers’ personality were not limited only to informational and methodological competences. The relevance of this research is to test the hypothesis about the emergence of a new type of teacher’s pedagogical culture, characterized by a special awareness of the humanistic and social role of a teacher in the course of e-learning. The aim is to study the content of a new type of pedagogical culture, as well as its structural components performed by the competences, the presence of which was actualized during the pandemic. The theoretical research methods (study and analysis of scientific publications on the teachers’ competences, especially in demand during the pandemic, synthesis, systematization, comparison, etc.) and empirical methods (conversation, questioning, observation) were used in the study. The conducted empirical research, as well as the analysis of domestic and foreign publications show that, despite a short-term nature of remote learning, the transformations in pedagogical culture have the character of a qualitative leap and are global in nature, as they have affected the teachers in all countries. This allows us to declare the emergence of a new type of pedagogical culture, namely the “electronic” pedagogical culture of a higher school teacher, which is a natural result of the development of “basic” culture and is characterized by a change in priorities in the structure and in the content of its constituent blocks of competences. The article compares real changes in the structure of a teacher’s personality with forecasts regarding the “competences of the future”, as well as with the “information pedagogical culture”, which is believed to have arisen in the process of information technologies penetration into the educational process. The scientific novelty of the research consists in substantiating the possibility of transforming the “basic” pedagogical culture of the teacher under the influence of external and internal factors. In theoretical terms, the study expands the scientific understanding of the essence of the teacher’s pedagogical culture, the patterns of its development and transformation, as well as its competence-based structural organization

Cellular immune response in infected mice to NSP protein encoded by the negative strand NS RNA of influenza A virus

Influenza A virus belongs to a family of enveloped viruses with an RNA genome of negative polarity consisting of 8 RNA segments. The transcription of this RNA genome results in the synthesis of positive-sense mRNAs that translate up to 16 unique viral proteins with the help of splicing and translational shift mechanisms. The 8th NS segment encodes the NS1 protein (27 kDa), which is an active interferon antagonist, and the nuclear export protein NEP (14 kDa) through the standard negative polarity pathway. In addition, an alternative open reading frame for the synthesis of a third viral protein (NSP, negative-strand protein) by means of a direct translation of genome polarity RNA (the so-called positive polarity genome strategy) was identified in the NS segment. Since it is unknown as to whether the NSP protein can be synthesized in the infected organism post viral infection, the generation of spleen leucocytes specific to this protein was studied in mice after two sequential infections with influenza A viruses of H1N1 and H3N2 subtypes. It was found that leucocyte clones specifically recognizing a peptide domain in the central region of the NSP protein (amino acid positions 82-119) were generated in mice infected with influenza A viruses. In silico prediction has shown strong major histocompatibility complex-1 (MHC-I) and MHC-II specific epitopes in this central domain of the NSP. Comparative analysis of the influenza H3N2 viruses circulating in humans during 1968-2018 has shown high NSP variability, which was similar to that shown for the hemagglutinin (HA) and neuraminidase (NA) proteins. The highest variability was found to be in the N- and C-terminal parts of the NSP. These observations suggest that synthesis of the NSP protein occurs in infected animals and further support a bipolar (ambisense) strategy of the RNA genome of human influenza A virus.Influenza A virus belongs to a family of enveloped viruses with an RNA genome of negative polarity consisting of 8 RNA segments. The transcription of this RNA genome results in the synthesis of positive-sense mRNAs that translate up to 16 unique viral proteins with the help of splicing and translational shift mechanisms. The 8th NS segment encodes the NS1 protein (27 kDa), which is an active interferon antagonist, and the nuclear export protein NEP (14 kDa) through the standard negative polarity pathway. In addition, an alternative open reading frame for the synthesis of a third viral protein (NSP, negative-strand protein) by means of a direct translation of genome polarity RNA (the so-called positive polarity genome strategy) was identified in the NS segment. Since it is unknown as to whether the NSP protein can be synthesized in the infected organism post viral infection, the generation of spleen leucocytes specific to this protein was studied in mice after two sequential infections with influenza A viruses of H1N1 and H3N2 subtypes. It was found that leucocyte clones specifically recognizing a peptide domain in the central region of the NSP protein (amino acid positions 82-119) were generated in mice infected with influenza A viruses. In silico prediction has shown strong major histocompatibility complex-1 (MHC-I) and MHC-II specific epitopes in this central domain of the NSP. Comparative analysis of the influenza H3N2 viruses circulating in humans during 1968-2018 has shown high NSP variability, which was similar to that shown for the hemagglutinin (HA) and neuraminidase (NA) proteins. The highest variability was found to be in the N- and C-terminal parts of the NSP. These observations suggest that synthesis of the NSP protein occurs in infected animals and further support a bipolar (ambisense) strategy of the RNA genome of human influenza A virus

Features of change of mechanical properties of precipitation hardening alloy 47ХНМ at hardening and ageing

Mechanical properties of nickel-chromic austenitic alloy 47ХНМ have been studied. It was shown that at increase of heating time for hardening the deforming pressures fall, plasticity grows that is connected with dissolution of excess a-phase. In order to obtain high strength properties with minimum level of elastic imperfections the thermal processing of alloy should be carried out in temperature range 650...750 °С, and time of ageing should be from 8 to 20 h depending on temperature of agein

Change of the spring Сr-Ni alloy microstructure after ageing

It has been stated that ageing of the tempered alloy 47ХНМ at temperature 500 °С during 5...10 h does not result in disintegration of oversaturated firm solution, at ageing temperature rise up to 600 °C attributes of disintegration in particles of ?-phase of homogeneoustype start to be shown. It was shown that after tempered samples ageing at 700 °C the faltering disintegration with allocation of not coherent ?-phase on the basis of chrome develops intensively, and its volume fraction increases with increase in ageing time reaching the maximal values in 5...10 h of agein

An investigation of the disinfection effect in joint action of the nanosecond electron beam and plasma radiation

The subject of the investigation of the possible synergy sterilizing effect included the joint action by the nanosecond electron beam (NEB) and plasma radiation of nanosecond gas discharge of high pressure. Plasma radiation was carried out by the GVI-150 generator which loading involved a discharge camera. Pulse repetition rate of the generator operation was 37 pps, and distance from electrodes cutoff to processed samples ∼5 cm. The experiments on irradiation by the NEB were made on the pulse repetitive nanosecond accelerator URT-0.5 (electrons energy up to 500 keV, a pulse duration 50 ns, pulse repetition rate up to 200 pps). The time the vessel has been irradiated changed from 0 to 5 minutes; the absorbed dose (AD) of different batches changed from 0 to 5 kGy. The extensive data array on joint action of the NEB and plasma radiation for several types of widespread microorganism is received. Also, the synergetic effect of the NEB influence and plasma radiation on microorganisms such as Klebsiella is found. © Published under licence by IOP Publishing Ltd.This work was performed within a support of the Russian Science Foundation No. 16-16-04038)

Relationship of erythrocyte adrenoreactivity with their quantitative and qualitative characteristics as a method for assessing the rheological properties of blood in athletes

Objective of the study: prediction of the rheological properties of blood in young men with different levels of motor activity (MA) by assessing the relationship between the adrenergic reactivity of erythrocytes (ARE) with their quantitative and qualitative parameters.Materials and methods: the study involved young men with a low (41 people) and a high level of physical activity (athletes — 20 people), aged 21–23 years. In all subjects, the total, individual characteristics of erythrocytes and ARE were determined. ERS was assessed by the change in the erythrocyte sedimentation rate (ESR) under the influence of adrenaline in vitro at final concentrations of 10–5; 10–6; 10–7; 10–8; 10–9; 10–11; 10–13 g/ml venous blood. According to the nature of the observed effect, in accordance with the direction of ESR shifts, 3 types of ARE were distinguished: increased ESR in the presence of adrenaline — aggregative (Ar, type 1); no changes — areactive (Ap, type 2); decreased ESR — antiaggregatory (AnAg, type 3).Results: in athletes, inverse correlations of the ARE types were established when exposed to stress concentrations of adrenaline (SCA) (above 10–8 g/ml) with the hemoglobin level (r = –0.59, p = 0.008), the average hemoglobin content in the erythrocyte (r = –0.55, p = 0.016), when exposed to physiological concentrations of adrenaline (PCA) (10–9 g/ml and below) — with the corpuscular volume of erythrocytes (r = –0.51, p = 0.029). In young men with a low level of MA, an inverse correlation was found between the minimum ARE values and the number of erythrocytes (r = –0.36, p = 0.01), and the maximum — with the average concentration of hemoglobin in the cell (r = 0.54, p = 0.04).Conclusion: in young men leading a sedentary lifestyle, the tendency of erythrocytes to aggregate under the influence of adrenaline increases with an increase in their number and intracellular viscosity. In athletes, the predominance of the antiaggregatory type of ARE, and, consequently, more stable rheological properties of erythrocytes and an improvement in microcirculation, are facilitated by a decrease in the size of erythrocytes and an average saturation of cells with hemoglobin

Atypical reactive center Kunitz-type inhibitor from the sea anemone <i>Heteractis crispa</i>

The primary structure of a new Kunitz-type protease inhibitor InhVJ from the sea anemone Heteractis crispa (Radianthus macrodactylus) was determined by protein sequencing and cDNA cloning. InhVJ amino acid sequence was shown to share high sequence identity (up to 98%) with the other known Kunitz-type sea anemones sequences. It was determined that the P1 Thr at the reactive site resulted in a decrease of the Ki of InhVJ to trypsin and a-chymotrypsin (7.38 × 10-8 M and 9.93 × 10-7 M, respectively). By structure modeling the functional importance of amino acids at the reactive site as well as at the weak contact site were determined. The significant role of Glu45 for the orientation and stabilization of the InhVJ-trypsin complex was elucidated. We can suggest that there has been an adaptive evolution of the P1 residue at the inhibitor reactive site providing specialization or functional diversification of the paralogs. The appearance of a key so-called P1 Thr residue instead of Lys might lead to refinement of inhibitor specificity in the direction of subfamilies of serine proteases. The absence of Kv channel and TRPV1-receptor modulation activity was confirmed by electrophysiological screening tests