Typical values for B6;129 and FVB V_TE mice.

<p>Values are given as the medians ± interquartile range (IQR) for WT and <i>cftr^-/-</i> B6;129 mice and WT and F508del-CFTR FVB mice. Inhibitory effects were assessed for Inh-172 and for DIDS. Mann-Whitney tests were used to compare groups.</p

Representative V_TE recordings.

<p>The recordings obtained with the final protocol are shown for WT (A) and <i>cftr</i>^-/- B6;129 mice (B). Three phases was observed: baseline V_TE after Cl^- solution perfusion, V_TEAmil. after the addition of amiloride (Amil.) and V_TELowCl^- after the replacement of Cl^- solution with a solution of low Cl^- concentration (LowCl^-). The inhibitory effect on Cl^- secretion of inhibitor-172 and inhibitor-172 plus DIDS was demonstrated in WT mice.</p

Parameters of the Bland-Altman plot.

<p>Values were determined for baseline potential difference (V_TE), amiloride response (ΔV_TEAmil.) and low Cl^- solution response (ΔV_TELowCl^-), from the Bland and Altman plot, for two measurements on the same 22 WT and 21 <i>cftr</i>^-/- mice.</p

Cutoff point determination for nasal potential difference parameters distinguishing between FVB WT and F508del-CFTR mice.

<p>Receiver operating characteristics (ROC) curves for (A) baseline potential difference (V_TE), (B) amiloride response (ΔV_TEAmil.) and (C) low Cl^- solution response (ΔV_TElowCl^-), for WT and F508del-CFTR FVB mice are shown. AUC: area under the curve; 95% CI: 95% confidence interval. Cutoff points were determined by the best positive likelihood ratio of sensitivity/(1-specificity).</p

Vaccination status according to A/H1N1 vaccination status.

<p>Vaccination status according to A/H1N1 vaccination status.</p

Measures of personal prevention.

<p>Measures of personal prevention.</p

Box plots corresponding to four peaks differentially displayed.

<p>Two sphingomyelin (m/z 489.3 and 725.5, upper panels) and two phosphatidylcholine (m/z 782.6 and 810.6, lower panels) species are represented. Relative intensity corresponds to the area of each peak of the spectrum related to the total ion count. Box plots are constructed from log-transformed relative intensity data.</p

Hierarchical clustering heat map representing increases (red) and decreases (red) in intensity of peaks significantly different in mild <i>vs</i> severe patients.

<p>Peak masses are represented in the vertical axis, and individuals in the horizontal axis. Data were log-transformed, obtained after clustering of peaks (vertical axis) and individuals (horizontal axis), analyzed by the Ward Agglomeration Algorithm, and filtered for mild <i>vs</i> severe patients comparison. The green trace delimits the data corresponding to the cluster of individuals that show consistent changes in lipid content.</p

Peaks differentially displayed in either healthy controls <i>vs</i> CF patients (*) or in CF mild <i>vs</i> CF severe patients (**) with p<0.05 after T-test analysis.

<p>All peaks are [M+H]⁺ except 489.27 and 725.53, which correspond to [M+Na]⁺. # Theoretical identity (not confirmed by MSⁿ).</p

Hierarchical clustering heat map representing increases (red) and decreases (blue) in intensity of peaks significantly different in healthy <i>vs</i> CF patients.

<p>Peak masses are represented in the vertical axis, and individuals in the horizontal axis. Data were log-transformed, obtained after clustering of peaks (vertical axis) and individuals (horizontal axis), analyzed by the Ward Agglomeration Algorithm, and filtered for healthy <i>vs</i> CF patients comparison. The green trace delimits the data corresponding to the cluster of individuals that show consistent changes in lipid content.</p