54 research outputs found

    Evaluation of Winter protein pea cultivars in the conditions of Serbia

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    There is a widespread opinion that the development of winter cultivars of protein pea could significantly increase its cultivation area, especially in the temperate regions. A small-plot trial was carried out on a chernozem soil at the Experiment Field of the Institute of Field and Vegetable Crops at Rimski Ĺ anÄŤevi, including six French and three Bulgarian winter protein pea cultivars. The cultivar 5105 had the earliest date of beginning of flowering (April 16) and the earliest date of harvest (June 11). The cultivars Dove and 5105 had the highest winter survival coefficients, with 0.93 each in the year of 2004/05 and 0.92 each in the year of 2005/06. In average, the highest grain yield was in the cultivars 5174 (6567 kg ha-1) and Dove (6453 kg ha-1) while the lowest grain yield was in the cultivar Frilene (1062 kg ha-1)

    Identification of Genes Differentially Expressed in Response to Cold in Pisum sativum Using RNA Sequencing Analyses

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    International audienceLow temperature stress affects growth and development in pea (Pisum sativum L.) and decreases yield. In this study, RNA sequencing time series analyses performed on lines, Champagne frost-tolerant and Térèse frost-sensitive, during a low temperature treatment versus a control condition, led us to identify 4981 differentially expressed genes. Thanks to our experimental design and statistical analyses, we were able to classify these genes into three sets. The first one was composed of 2487 genes that could be related to the constitutive differences between the two lines and were not regulated during cold treatment. The second gathered 1403 genes that could be related to the chilling response. The third set contained 1091 genes, including genes that could be related to freezing tolerance. The identification of differentially expressed genes related to cold, oxidative stress, and dehydration responses, including some transcription factors and kinases, confirmed the soundness of our analyses. In addition, we identified about one hundred genes, whose expression has not yet been linked to cold stress. Overall, our findings showed that both lines have different characteristics for their cold response (chilling response and/or freezing tolerance), as more than 90% of differentially expressed genes were specific to each of them

    Etude du comportement de deux varietes precoces de soja (Glycine max L. Merrill, cv SITO et FISKEBY) dans le nord de la France

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    SIGLEAvailable from INIST (FR), Document Supply Service, under shelf-number : T 82600 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

    RĂ©sistance au gel. Conditions d'acclimatation au froid

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    Acclimation of Medicago truncatula to cold stress

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    Acclimation of Medicago truncatula to cold stres

    Le pois d'hiver : une solution pour Ă©chapper aux stress climatiques et biotiques

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    National audienceLes pois de printemps, semés en fin d’hiver, sont plus exposés aux ravageurs et àAphanomyces. L’intérêt des pois d’hiver, semés à l’automne, est d’allonger le cycle, etainsi d’augmenter a priori le potentiel de rendement et d’avancer les stades sensiblesaux stress de fin de cycle vers des périodes moins exposées. Mais ce type de pois estconfronté au gel hivernal

    Pois d'hiver. La résistance au froid bientôt décryptée

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    BAPGEAPSIPois d'hiver. La résistance au froid bientôt décrypté

    Mise en évidence de la synténie de QTL de tolérance au gel sur les groupes de liaison VI chez Pisum sativum (WFD 6.1) et Medicago truncatula (Mt-FTQTL6) et cartographie fine de Mt-FTQTL6

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    L identification des bases moléculaires de la tolérance au gel présente une grande importance tant sur le plan fondamental qu appliqué. Medicago truncatula est une légumineuse modèle pour les espèces tempérées. Un QTL majeur de tolérance au gel après acclimatation au froid (Mt-FTQTL6), expliquant 40% de la variation phénotypique, a été détecté sur le chromosome 6 de cette espèce. En parallèle, un QTL pour le même caractère (WFD 6.1/FD164.c) a été identifié sur le groupe de liaison équivalent chez Pisum sativum. Cette thèse a pour objectifs de confirmer la synténie des régions chromosomiques contenant Mt-FTQTL6 et WFD 6.1/FD164.c et d identifier des gènes candidats positionnels pour Mt-FTQTL6. Les premiers efforts ont permis de localiser Mt-FTQTL6 dans un intervalle de 3,7 cM qui coïncide avec une zone du génome de Medicago truncatula dont l assemblage reste incomplet. Les ressources génomiques de Glycine max ont été ensuite exploitées. Cinq marqueurs géniques ont permis d'ancrer les régions chromosomiques de Mt-FTQTL6 et WFD 6.1/FD164.c. Des clones BAC correspondant à 15 marqueurs (sondes) ont été assemblés en 6 contigs couvrant l intervalle de confiance de Mt-FTQTL6. Des lignées F7 ou F8, recombinantes au niveau de cet intervalle, ont été identifiées et phénotypées pour la tolérance au gel en conditions contrôlées. L'intervalle de confiance de Mt-FTQTL6 a ainsi été réduit à une région de 0,4 cM contenant 20 gènes parmi lesquels 12 gènes CBF/DREB1 en tandem. La variation allélique pour 11 gènes CBF/DREB1 a été mise en évidence chez les parents de la population de cartographie.La validation fonctionnelle est maintenant envisageable chez Medicago truncatula et Pisum sativum.Unraveling the molecular bases of freezing tolerance is of great importance both at the fundamental and applied levels. Medicago truncatula is a model legume for studies concerning cool-season species. A major freezing tolerance QTL after cold acclimation (Mt-FTQTL6), accounting for 40% of the phenotypic variation, has been identified on chromosome 6 of this species. Interestingly, a QTL for the same trait has been mapped on the corresponding linkage group in Pisum sativum (WFD 6.1/FD164.c). The present thesis aimed to confirm synteny between Mt-FTQTL6 and WFD 6.1/FD164.c harboring regions and to subsequently identify positional candidate genes for Mt-FTQTL6. Using BAC-derived markers, Mt-FTQTL6 has been first located in a 3.7-cM interval, coinciding with an assembly physical gap. Mt-FTQTL6 co-orthologous blocks in Glycine max were identified and exploited to develop additional markers. Five common gene-based markers were obtained between Mt-FTQTL6 and WFD 6.1/FD164.c chromosomal regions. Positive BAC clones for 15 different markers (probes) were assembled in 6 BAC contigs linked to Mt-FTQTL6. Homozygous F7 or F8 recombinant lines at Mt-FTQTL6 were identified and evaluated for freezing tolerance under controlled conditions. The QTL confidence interval was subsequently delimited to a 0.4 cM-region that contains 20 protein-coding genes including 12 tandemly-arrayed CBF/DREB1 genes. Isolation of 11 out of the 12 CBF/DREB1 genes from both parents of the mapping population was successfully achieved. Efforts will be next needed for functional validation in Medicago truncatula and Pisum sativum.LILLE1-Bib. Electronique (590099901) / SudocSudocFranceF
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