Isolation and characterization of mesotrione-degrading Bacillus sp. from soil

International audienceDissipation kinetics of mesotrione, a new triketone herbicide, sprayed on soil from Limagne (Puy-de-Dôme, France) showed that the soil microflora were able to biotransform it. Bacteria from this soil were cultured in mineral salt solution supplemented with mesotrione as sole source of carbon for the isolation of mesotrione-degrading bacteria. The bacterial community structure of the enrichment cultures was analyzed by temporal temperature gradient gel electrophoresis (TTGE). The TTGE fingerprints revealed that mesotrione had an impact on bacterial community structure only at its highest concentrations and showed mesotrione-sensitive and mesotrione-adapted strains. Two adapted strains, identified as Bacillus sp. and Arthrobacter sp., were isolated by colony hybridization methods. Biodegradation assays showed that only the Bacillus sp. strain was able to completely and rapidly biotransform mesotrione. Among several metabolites formed, 2-amino-4-methylsulfonylbenzoic acid (AMBA) accumulated in the medium. Although sulcotrione has a chemical structure closely resembling that of mesotrione, the isolates were unable to degrade i

Dissolved organic matter does not promote glyphosate degradation in auto-heterotrophic aquatic microbial communities

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Identification des gènes et enzymes impliqués dans la dégradation de l’herbicide mésotrione chez Bacillus megaterium Mes11

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Growth of Pseudomonas species isolated from Algerian agricultural soils in the presence of Abamectin

International audienceAbamectin is a broad spectrum highly effective acaricide, insecticide, and antiparasitic agent. Studies aboutthe microbial degradation of abamectin are still rare and only three abamectin-degrading bacterial strainswere isolated until now. In this paper, we reported the isolation and molecular characterization of bacterialstrains isolated from three soils located in eastern Algeria, and the evaluation of their ability to grow in thepresence of abamectin. Out of a total of 48 Gram negative and Gram positive bacterial strains identified,seven strains were able to develop on agar in the medium of mineral salt different concentrations of thetested insecticide. The four bacteria showing the best growth capacity were identified as being Pseudomonasmediterranea isolate PSB47, Pseudomonas sp. OX1, Pseudomonas migulae strain OKB3, and Pseudomonas sp.clone 6A4 and were tested for their ability to grow in the presence of abamectin as the only energy andcarbon sources. In general way, these strains showed a slow but a significant growth visualized by themonitoring of absorbance at 600 nm, suggesting they could be used for bioremediation of polluted soil withabamectin