1 research outputs found
Hydroxyapatite-Gold Modified Screen-Printed Carbon Electrode for Selective SARS-CoV‑2 Antibody Immunosensor
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),
or
coronavirus disease 2019 (COVID-19), is still spreading worldwide;
therefore, the need for rapid and accurate detection methods remains
relevant to maintain the spread of this infectious disease. Electrochemical
immunosensors are an alternative method for the rapid detection of
the SARS-CoV-2 virus. Herein, we report the development of a screen-printed
carbon electrode immunosensor using a hydroxyapatite-gold nanocomposite
(SPCE/HA-Au) directly spray-coated with the immobilization receptor
binding domain (RBD) Spike to increase the conductivity and surface
electrode area. The HA-Au composite synthesis was optimized using
the Box–Behnken method, and the resulting composite was characterized
by UV–vis spectrophotometry, TEM-EDX, and XRD analysis. The
specific interaction of RBD Spike with immunoglobulin G (IgG) antibodies
was evaluated by differential pulse voltammetry and electrochemical
impedance spectroscopy methods in a [Fe(CN)6]4–/3– solution redox system. The IgG was detected with a detection limit
of 0.0561 pg mL–1, and the immunosensor had selectivity
and stability of 103–122% and was stable until week 7 with
the influence of storage conditions. Also, the immunosensor was tested
using real samples from human serum, where the results were confirmed
using the chemiluminescent microparticle immunoassay (CMIA) method
and showed satisfactory results. Therefore, the developed electrochemical
immunosensor can rapidly and accurately detect SARS-CoV-2 antibodies