Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization-1

<p><b>Copyright information:</b></p><p>Taken from "Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization"</p><p>http://www.biomedcentral.com/1472-6750/7/80</p><p>BMC Biotechnology 2007;7():80-80.</p><p>Published online 21 Nov 2007</p><p>PMCID:PMC2200642.</p><p></p>e were analyzed. 1, 2, 3 – 0.5, 2.0 and 5.0 μl of the DNA probe isolated from clone 1 of MG1655/RSF1010; 4, 5 – 2.0 μl of the DNA probes isolated from clones 2 and 3 of MG1655/RSF1010; 6, 7, 8 – 2.0 μl of the DNA probes isolated from 3 independent clones of MG1655/RSFmob; 9, 10, 11 – 2.0 μl of the DNA probes isolated from 3 independent clones of MG1655/RSFmob-I. Relative copy number was calculated as the ratio of the DNA amounts in the upper bands (5.9 kb EcoRV-EcoRV fragment). Electrophoretic patterns for stationary phase are represented as an example