Isometric embeddings in bounded cohomology

This paper is devoted to the construction of norm-preserving maps between bounded cohomology groups. For a graph of groups with amenable edge groups we construct an isometric embedding of the direct sum of the bounded cohomology of the vertex groups in the bounded cohomology of the fundamental group of the graph of groups. With a similar technique we prove that if (X,Y) is a pair of CW-complexes and the fundamental group of each connected component of Y is amenable, the isomorphism between the relative bounded cohomology of (X,Y) and the bounded cohomology of X in degree at least 2 is isometric. As an application we provide easy and self-contained proofs of Gromov Equivalence Theorem and of the additivity of the simplicial volume with respect to gluings along pi_1-injective boundary components with amenable fundamental group

Immunological fingerprint of 4CMenB recombinant antigens via protein microarray reveals key immunosignatures correlating with bactericidal activity

Serogroup B meningococcus (MenB) is a leading cause of meningitis and sepsis across the world and vaccination is the most effective way to protect against this disease. 4CMenB is a multi-component vaccine against MenB, which is now licensed for use in subjects >2 months of age in several countries. In this study, we describe the development and use of an ad hoc protein microarray to study the immune response induced by the three major 4CMenB antigenic components (fHbp, NHBA and NadA) in individual sera from vaccinated infants, adolescents and adults. The resulting 4CMenB protein antigen fingerprinting allowed the identification of specific human antibody repertoire correlating with the bactericidal response elicited in each subject. This work represents an example of epitope mapping of the immune response induced by a multicomponent vaccine in different age groups with the identification of protective signatures. It shows the high flexibility of this microarray based methodology in terms of high-throughput information and minimal volume of biological samples needed