Kaplan<i>-</i>Meier survival curves in the E1 phase (left) and E2 phase (right).

<p>The complete data relates to the death snails, whereas the censored data are associated with the living individuals.</p

Mean (with SE) manganese concentrations (mg/Kg d. wt).

<p>A) soil; B) snail hepatopancreas; C) foot; D) shell. Marked boxes (*) indicate significant differences as compared to the reference group of each experimental phase (Duncan’s test, p<0.05).</p

The soil-to-hepatopancreas regressions in the E1 and E2 phases.

<p>The scatter plots correspond to the mean manganese concentrations in the soil and hepatopancreas (triplicate determinations).</p

Mean (and SE) for shell height in snails exposed to Mn-spiked soil.

<p>The values were measured at the start of the experiment (day 0), end of E1 phase (day 30), and end of E2 phase (day 60).</p

Snail survival in percentage and mean survival time (and SE) for each Mn treatment.

<p>The values were measured at the end of E1 phase (day 30) and E2 phase (day 60).</p

Effect of dietary cadmium on genome-wide 5mC levels in DNA of the hepatopancreas of <i>C</i>. <i>aspersus</i> adults.

<p>(<b>A</b>) The mean contents of 5mC in hepatopancreas DNA in each treatment group at each time point. (<b>B</b>) Estimated marginal means for dietary dose (as the main effect). (<b>C</b>) Estimated marginal means for time (as the main effect). Four snails were sampled for each treatment group at each time point (14, 28, and 56 days). Data are shown on a log₁₀ scale as mean (point) with one standard error (box) and one standard deviation (error bar). In Fig 2A the outliers (white circles) and the extremes (whiskers) are also given (if they exist). Marked boxes(*) indicate significant differences as compared to the reference group (Newman-Keuls test, ***—<i>p</i> < 0.001, **—<i>p</i> < 0.01, *—<i>p</i> < 0.05).</p

Kaplan-Meier survival curves in the E1 phase (left) and E2 phase (right).

<p>The complete data relate to the death snails, whereas the censored data are associated with the living individuals.</p

Mean (and SD) for SW in snails exposed to Cd-spiked soils.

<p>Marked boxes (*) indicate significant differences as compared to the reference group (Dunnet’s test, p < 0.05).</p><p>Mean (and SD) for SW in snails exposed to Cd-spiked soils.</p

Photograph of different types of shell damages (indicated with arrows) in <i>C. aspersus</i> sub-adults.

<p>A) Irregular edges around the aperture; B) Shell erosion along the suture line; C) Thinned, fragile, and irregular edges around the aperture and broken apex.</p

Survival times and mortalities for each time period.

<p>Survival times and mortalities for each time period.</p